Abstract
The Marsh Fritillary butterfly (Euphydryas aurinia) is a Eurasian species which has suffered significant reductions in occurrence and abundance over the past century, particularly across the western side of its range, due to agricultural intensification and habitat loss. This loss has been particularly severe in the UK with extensive localised extinctions. Following sympathetic management, reintroduction was undertaken at four Cumbria (northern UK) sites in 2007 with stock from a captive admixture population descended from Cumbrian and Scottish founders. Annual population monitoring of the reintroductions was undertaken. Nine years post-reintroduction, the level of population genetic variation was assessed using microsatellites. Variation in historical Cumbrian samples was determined using museum samples and Scottish samples from current populations were assayed to characterise natural population variation. Half of the Scottish sites also served as indicators of the alleles present in the founder populations. The genetic contribution of the founder populations allied to population size data allowed patterns of genetic variation to be modelled. Alleles from Cumbrian and Scottish founders are present in the reintroduced populations. The four sites have levels of variation akin to natural populations and exhibit differentiation as predicted by statistical modelling and comparable with natural populations. This suggests that reintroduction following captive breeding can produce self-sustaining populations with natural levels of genetic diversity. These populations appear to be undergoing the same evolutionary dynamics with bottlenecks and drift as natural populations.
Implications for insect conservation
Reintroduction of captive bred individuals is a viable strategy for producing populations with natural levels of genetic diversity and evolutionary dynamics. Hybridisation of populations on the brink of extinction with those thriving can preserve some of the genetic distinctiveness of the declining population.
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Introduction
Reintroductions are commonly used in conservation to offset the ongoing decline in biodiversity (Seddon et al. 2007, 2012). Historically these were typically undocumented in the scientific literature (Schultz et al. 2008). However, this has changed since the turn of the millennium with reintroductions being the focus of various studies including monitoring (Wakamiya and Roy 2009; Bernardo et al. 2011; Nichols and Armstrong 2012), management (Jones and Merton 2012; West et al. 2017), range expansion (Halley et al. 2012; Gaywood 2018) and population modelling (for summary see Armstrong and Reynolds 2012). The primary measure of the success of reintroductions is generally considered to be the establishment and successful breeding of a population and typically this is monitored for just a few years or until the emergence of the F1 or F2 generation (Wauters et al. 1997; Spalton et al. 1999; Richards and Short 2003; Sarrazin et al. 2008; Godefroid et al. 2011; Sanz and Grajal 2010).
Less commonly considered as a measure of success is the population genetic variation following reintroduction. Given that genetic variation provides the raw material upon which natural selection acts such variation is essential for the survivorship of a population (Lacy 1997). In the short term, a lack of genetic diversity causes inbreeding depression and other associated problems which can lead to population declines and increased extinction risk. In the long term it limits the ability of a species to adapt to changes in the environment such as climate or a novel pathogen (Allendorf et al. 2013). However, there is a notable absence of genetic considerations of reintroduction success in the literature.
Genetic assessments that do exist usually compare reintroduced and captive populations such as those in the lesser kestrel (Falco naumanni) (Alcaide et al. 2010); the black-footed ferret (Mustela nigripes) (Wisely et al. 2008); American martens (Martes americana) (Hillman et al. 2017) and Griffon vulture (Gyps fulvus) (Le Gouar et al. 2008). Such studies have also been used to infer historic genetic patterns, including bottleneck events from modern populations (e.g., Alpine ibex (Capra ibex ibex) Biebach and Keller 2009) and to assess gene flow between reintroduced and natural populations (e.g., in eastern wild turkeys (Meleagris gallopavo silvestris), Latch and Rhodes 2005). Such examples reflect the vertebrate bias in the reintroduction literature (Groombridge et al. 2012). By comparison invertebrate reintroductions are rarely studied. Of the 349 reintroduction case studies published by the IUCN only 28 (~ 8%) feature invertebrates (Soorae 2018) and fewer still have assessed the genetic diversity of reintroduced invertebrate populations. From a population genetic and modelling perspective this lack of invertebrate studies is a lost opportunity given the vagility, discrete generation time and population monitoring possible with some invertebrates, most notably butterflies.
Within the UK there is a long, though poorly documented, history of amateur invertebrate reintroductions in the UK, mostly of butterfly species (Schultz et al. 2008).The majority of these reintroductions failed due to insufficient planning, poor habitat quality at release sites, limited release numbers and incomplete understanding of species’ biology (Schultz et al. 2008). Nevertheless there have been successful invertebrate reintroductions in the UK, the best known being that of the Large Blue butterfly (Phengaris (= Maculinea) arion). Following extinction of the endemic UK subspecies P. a. eutyphron in 1979, reintroduction was undertaken in 1984 with the Swedish subspecies (P. a. arion) following research to understand the species’ life history and management requirements (Thomas et al. 1989). Further UK reintroductions of the Large Blue followed and the species is currently considered to be established and breeding in the UK (Thomas et al. 2009). Moreover after 19 generations the UK populations are now genetically differentiated from the Swedish donor populations and show differentiation between separate UK populations (Andersen et al. 2014).
While the Large Blue was always a species with limited distribution (Ford 1945; Asher et al. 2001), more widely dispersed butterfly species have experienced precipitous declines in the UK over the last 30 years prompting significant conservation concern. One such species is the Marsh Fritillary (Euphydryas aurinia), a univoltine species forming conspicuous larval webs in the early autumn and spring, found throughout much of Europe and presently classified as Least Concern by the IUCN, reflecting its broad geographical range. However it is declining throughout its range and regionally extinct in certain areas (van Swaay et al. 2010). The British Isles has been identified as a stronghold of the species with up to 15% of the European population. It is legally protected within Europe under the EU Habitats Directive, and in the UK since 1998 under the Wildlife and Countryside Act 1981.
Despite legal protections and conservation efforts it has still undergone significant declines in Britain over the past thirty years. This is due to changes in agricultural practices, including enrichment of pastures (Porter and Ellis 2011), leading to a decrease in suitable habitat for the larval food plant, Devil’s-bit Scabious (Succisa pratensis) (Ridding et al. 2015).
One of the most heavily studied and historically important Marsh Fritillary populations was in Cumbria, in the north-west of England where it was widely dispersed (Ford and Ford 1930; Ford 1945; Porter and Ellis 2011). In the time before agricultural intensification, it was described as experiencing cyclical fluctuations in population size (Ford and Ford 1930). However from the 1980s it began to decline irreversibly and increasing local extinctions were noticed (Porter and Ellis 2011). The decline continued despite attempts to reverse it through management practices and two failed reintroductions (Porter and Ellis 2011).
By 2004, one population remained in Cumbria and only a single female was seen. Extensive surveying in autumn 2004 found just one larval web and the larvae were then taken into captivity with the intention of future reintroduction. As the larvae were the offspring of a single female and male, there was a concern that the resulting captive population would suffer from inbreeding depression. Hence the captive population was bred with individuals from the Argyll region of Scotland. This region was chosen because it housed healthy populations and the suggestion of historic gene flow between these populations and the Cumbrian (Porter and Ellis 2011). Thus, the captive Cumbrian adults were crossed with 95 individuals from 19 sites across the Argyll region (five larvae per site).
Reintroductions of the admixture population were carried out in 2007 across four sites (total 42,000 3rd stage instar larvae, ~ 10,000 per site) which had been sympathetically managed prior to reintroduction (for details see (Porter and Ellis 2011)). Annual August population counts of larval webs reveal that all four reintroduction sites have persisted and are established (Porter and Ellis 2011). This persistence of the reintroductions implies success. However, their current genetic composition was unknown prior to this study and the establishment of four separate populations from the same initial genetic stock within a decade provides the opportunity to investigate the genetic differentiation of these populations. Moreover, the annual population estimates allied to the life history characteristics of the butterfly enable patterns of empirical population differentiation to be assessed against modelled variation.
An additional asset is the existence of historic Cumbrian specimens held in museums and private collections which date from both the pre-decline period (1920-1930s) and the final decline (1970–1993). These specimens present an opportunity to place the current level of population genetic variation in a historical context. A geographical and evolutionary context is possible by comparison of the reintroduced admixture that has formed the current population with that from the areas where the Scottish founder stock originated. The combination of historical specimens prior to local extinction, knowledge of the genetic constitution of the reintroduced populations, and samples from the areas that partly provided the material for the reintroductions, samples from current populations a decade after reintroduction alongside records of annual population estimates presents a unique opportunity to examine the fate of genetic variation in a reintroduced species. This study aims to determine: (1) if the four populations at the four original reintroduction sites have levels of genetic variation comparable with natural populations (2) how the genetic diversity of the reintroduced populations compares with both the historic one and with the Scottish donor populations. (3) the level of genetic differentiation between the four reintroduction sites after nine generations, 4) whether patterns of genetic variation in the reintroduced populations match the patterns predicted by computer modelling.
To the authors knowledge, this will be the first time a study has compared the genetic structure of an admixture reintroduced butterfly population with its two donor populations, one of which was the historic population at the reintroduction site. This will also be the first time that the genetic diversity of a reintroduced population has been compared to expected patterns of genetic diversity derived by computer modelling and based upon real population counts.
Methods and materials
Site selection and field sample collection
The sites used in this study are the four reintroduction sites described in Porter and Ellis (2011) (Fig. 1); due to the vulnerability of the sites to collection and the legal protection of the species the site names used to anonymise locations in that study will be retained here. Samples of larvae were collected in August 2016 under Natural England licence number 2016–25,165-SCI-SCI. A single larva per web was taken to prevent the collection of closely related individuals and a maximum of 30 larvae per site were collected as this was anticipated to give a fair representation of the genetic diversity at each site (Hale et al. 2012; Smee 2011).
Samples were collected from Scotland in August and September 2017 (under Scottish Natural Heritage licence number 104772) from four sites in the Argyll region (Fig. 2). It was not possible to sample the precise donor location due to the time that had elapsed since they were collected and the intermittent site occupancy by the species, given its metapopulation ecology (Junker et al. 2021). However, the broad geographical areas were known and two populations within these areas (Tayvallich and Mull) were sampled to represent the original Scottish donors. Two additional populations within the Argyll region but outside the original donor region (Islay and Oronsay) were also sampled for comparison. Sample collection was carried out as in Cumbria.
The original Cumbrian population was represented by historic specimens from the 1920–1930s held in museum collections (hereafter designated Cumbria Historic), plus specimens from the 1970–1990s and held in a private collection (designated Cumbria Donor), were sampled by removal of a leg with entomological forceps with permission from the collectors or museum curators. These specimens were assigned to a geographical area based on the location information provided with the specimen (Fig. 1).
DNA extraction and amplification
Field collected specimens were stored at − 80 °C until used. DNA was extracted from larval heads using DNeasy Blood and Tissue kit (Qiagen), following the standard protocol supplied by the manufacturer. For museum samples, DNA was extracted from the removed leg following the same protocol but with increased lysis time (12 h instead of 3 h).
This work was undertaken using microsatellites. These are frequently used in invertebrate population genetics studies (Meng et al. 2008; Sigaard et al. 2008; Smee et al. 2013; Bogdanowicz et al. 2015; Nakahama and Isagi 2018) and utilise existing techniques to successfully extract DNA from preserved museum species (Harper et al. 2006; Wandeler et al. 2007; Watts et al. 2007).
Genotypes of all samples were scored using five microsatellites (Smee et al. 2013: Aurinia_01, Aurinia_13, Aurinia_45 and Aurinia_70; Sinama et al. 2011; Eau88), forward primers were fluorescently tagged with 6-FAM (Integrated DNA Technologies). PCR was carried out in 20 μl reactions containing: 1.0 μl template DNA, 4.0 μl 5xPhusion High-Fidelity buffer (Thermo Scientific), 0.2 μl Phusion DNA polymerase (Thermo Scientific), 4 μl 10 mM dNTP mix (containing 2.5 mM of each) (Bioline), 0.6 μl DMSO, 1 μl 10 nM of each primer, 2.5 μl 10 nM MgCl2. Amplifications were carried out according to the following thermal profiles. For primers from Smee et al (2013): denaturing at 95 °C for 5 min; 25 cycles at 95 °C for 5 s, 60 °C (62 °C for Aurinia_16) for 30 s, 68 °C for 1 min; followed by 8 cycles at 95 °C for 5 s, 53 °C (55 °C for Aurinia_16) for 30 s, 68 °C for 1 min; and a final extension step of 72 °C for 30 min. For Eau88: denaturing at 95 °C for 15 min; 30 cycles of 94 °C for 1 min, 56 °C for 1 min, 72 °C for 1 min; and a final extension step of 60 °C for 45 min.
PCR products were separated via capillary electrophoreses on an AB3500 Genetic Analyser (Applied Biosystems) and sized relative to an internal size standard (LIZ500; Applied Biosystems) using GeneMapper 5. Sizes were checked manually and individuals with unclear peaks were amplified and genotyped again. Due to the degraded nature of the preserved collection specimens, slightly greater leeway was allowed when genotyping, provided that clear peaks could be identified. A subset incorporating both field and museum samples were re-genotyped to ensure accuracy. Raw allele scores were binned using TANDEM to reduce error in the binning process.
Statistical analysis
The statistical power of the microsatellites to detect population differentiation was assessed in POWSIM 4.1 (Ryman and Palm 2006) using allele frequencies for the total dataset. FST values were generated using an effective population size of 1000 individuals with 2000 iterations. The test showed the probably of detecting FST of 0.01 or greater was 81% while the probability of detecting FST of 0.02 or greater was 100%.
Data analysis was carried out in R version 3.3.2 (R Core Team 2021) using binned allele sizes unless otherwise stated. To characterise the genetic variation within populations’ basic population statistics were calculated using diveRsity (Keenan et al. 2013). Pairwise FST and Nei’s G’ST were calculated with corresponding p-values calculated using strataG to determine the level of differentiation between populations. Principle Component Analysis was used to analyse genetic structure of the reintroduced population with reference to the donor populations, this has been demonstrated to be a suitable analysis technique in admixture populations (Ma and Amos 2012). The analysis was carried out in Gstudio (Dyer 2012) and visualised with ggplot2 (Wickham 2009).
Modelling
As an aid in inference a forward-time population genetic simulation was written in Python using the simuPOP module (Peng and Kimmel 2005). This recreated the demographic history of the populations under study as closely as possible, but did not include mutation, selection or gene flow. Similar results to the empirical data will suggest that drift alone is enough to generate the population differentiation observed. A total of 1000 replicate simulations were run.
Simulation outline
The simulation used five loci with similar levels of genetic variation to the empirical data. This utilised an initial nineteen source populations each with 104 individuals and a 2:1 male: female sex ratio (based on the work of Schtickzelle et al. (2005) and Zimmermanet al. (2011a)) All loci were assigned five alleles randomly selected from a list of possible allelic states based on the empirical data for source populations (eight to twelve, depending on locus). Frequencies of each allele were also randomly assigned. A twentieth population with only one male and one female individual was also created to represent the Cumbrian remnant. Loci therein were randomly assigned only three alleles, again with randomly generated frequencies. Source populations underwent a bottleneck down to five individuals and were merged before breeding began (for a total of 97 individuals at generation zero). For two generations thereafter, random monogamous mating events occurred, each producing 300 offspring (with a 66% chance of being male), until a carrying capacity of 5 × 104 individuals was reached. Prior to the fourth generation’s mating events, the population was split into four groups of 1.5 × 104 individuals, representing the initial Cumbrian reintroductions (the remaining 8 000 were discarded from the model). After this, random mating proceeded for a further twelve generations, but the number of mating events varied according to empirical census data (Fig. 5b). That is, mating events occurred with 30 offspring each until the simulated population sizes matched the observed number of webs that year multiplied by 30 (giving a 10% survival rate based on larval survival rates in related Euphydryas species (Ehrlich et al. 1975; Kuussaari et al. 2004) and taking into account the reported effects of parasitoid attack (Porter 1981; Bulman 2001) which is assumed to be absent due the extinction of the parasitoid in Cumbria following the extinction of the host population).
However, census numbers for Site M post-generation three were too low to produce enough offspring to reach the desired size in generation five. Therefore, this number was increased to five individuals to allow the simulation to proceed. Similarly, one run of the simulation (0.1%) was aborted due to a lack of mate availability, which meant that with a fixed number of offspring and monogamous mating, there were insufficient pairings to produce a target population size. As the goal was to assess the potential impacts of a specific demographic history on genetic variation, that run was therefore discarded.
All model populations were randomly sampled at each generation (n = 30), a sample size comparable to the empirical data. Median heterozygosity across loci—both observed and expected – was determined across sites, and finally across runs. Median pairwise FST between populations was also calculated from these samples (Weir and Cockerham 1984).
Results
Reintroduced population genetic diversity
All Cumbrian reintroduction sites showed significant heterozygote deficiency (Table 1). Private alleles were observed at each site, accounting for 7.14–10.71% of observed alleles at a regional level. No significant level of inbreeding (FIS) was detected. Pairwise FST values (Table 2) are mostly significant, the exception is between Site B and Site M which is non-significant. G’ST values, which are standardised to take into account variation in diversity at different loci, show a similar pattern to the FST scores.
The Principal Component Analysis (Fig. 3) showed no clear separation between all four reintroduced populations. However, Site M shows little overlap with Site F and Site E. Site B shows extensive overlap with both Site F, Site E and Site M, reflected in the lowest pairwise FST values and the lack of significant FST between Sites B and M.
Comparison with founder populations
The Cumbrian reintroduction sites show higher allele number, richness and FIS compared with the Scottish populations (Table 1). Scotland shows a higher occurrence of private alleles than Cumbria, but Cumbria does contain private alleles which are absent from the Scottish populations. The original Cumbrian population also contains private alleles absent from the reintroduced population or Scotland. When the time periods are considered separately, it can be seen that the original Cumbrian private alleles are restricted to the earlier time period (1920–1930). Nevertheless, the high incidence of shared alleles leads the two time periods to largely cluster together on the PCA (Fig. 4).
Scottish donor and non-donor sites showed similar levels of diversity and variation. Pairwise FST and G’ST values between the Cumbrian reintroduced population and the donor populations are lower than between Cumbria and non-donor populations (Table 3).
PCA comparison between the reintroduced population and the populations which represent the two parts of the founder population show that separation between all three groups is almost complete (Fig. 4). The Scottish samples are separated completely from all Cumbrian material on PC1. The reintroduced population is distinct from the historic Cumbrian populations on the PC2 axis, with the exception of one point. There is no separation between donor and non-donor sites in the Scottish clustering and both show comparable levels of genetic diversity.
Simulation
Simulations generated broadly similar levels of genetic diversity and population differentiation as those estimated from empirical data (Fig. 5a, c). Median observed heterozygosity was consistently higher than expected from the simulation (Fig. 5c; Table 1). Overall median pairwise FST increased as the simulations progressed and at the equivalent generation (12) was slightly lower than that calculated in this study (FST = 0.031 and FST = 0.066, respectively; Fig. 5a; Table 2). Differentiation was however variable between runs, ranging from effectively identical (less than zero) to 0.237 by the end of the simulation (Fig. 5a). The smallest population, Site M, had the highest levels of differentiation consistently (throughout all replicates) after only one generation post-reintroduction (Fig. 5a).
Discussion
The ultimate measure of success of a reintroduction programme is to restore populations that exhibit sufficient evolutionary dynamism to survive pressures from both natural selection and stochastic processes (Seddon et al. 2012; Armstrong and Reynolds 2012; Robert et al. 2015). From a variety of perspectives the Cumbrian Marsh Fritillary reintroduction programme meets this criterion of success. All four of the reintroduction sites display similar levels of genetic variation. These are greater than the levels of variation displayed in the four Argyll sites. This may be due to the diverse origins of the reintroductions, incorporating nineteen Scottish sites plus the captive Cumbrian individuals. Comparable high levels of genetic diversity in translocated populations were found in the hihi (Notiomystis cincta), a New Zealand endemic bird (Brekke et al. 2011).
Genetic swamping, the total replacement of the local genotypes with incoming genotypes (Lenormand 2002) is a concern with some reintroduced populations (Veale and Russello 2016). Despite the disproportionate number of alleles contributed by the Scottish founders, potentially a maximum of 190 alleles per locus compared to maximum of four per locus for the Cumbrian founders, this has not occurred in the Cumbrian reintroduction. The clear separation of the reintroduced and Scottish populations in the PCA plot, alongside the slight overlap on the PC2 axis, between the historic Cumbrian and reintroduced populations, provides evidence of the persistence of Cumbrian specific alleles.
The occurrence of 15.78% private alleles in the reintroduced Cumbrian populations, absent from the Argyll sites may be due to one or more of three factors: mutation in the markers; the alleles are at low frequency in Argyll and were not detected in this survey, or the alleles are unique to Cumbria and demonstrate the persistence of the original Cumbrian population, either in the form of alleles preserved in the captive population or in previous undetected wild populations that have interbred with the reintroduced populations. Despite the relatively high mutation rate of microsatellites the low generation number makes it unlikely that this phenomenon will account for this genetic distinctiveness (Ellegren 2009; Bhargava and Fuentes 2010).
The persistence of a previously undetected Cumbrian population of sufficient size to contribute such an amount of private alleles is highly unlikely given the close monitoring of the species in the region over the decades from decline to extinction (Porter and Ellis 2011). There are also no reported occurrences of the species outside of the reintroduction sites or adjacent areas (CMFAG, pers. comm.). Nor is it possible that these are entirely due to the contribution of the Cumbrian founders to the captive breeding, as these are all suspected to be the offspring of a single pair (Porter and Ellis 2011) and thus at most only a few alleles per locus could have been contributed. Given that the populations had been declining for a number of years it is likely that the actual number of unique alleles contributed by the Cumbrian founders was markedly less due to the effects of inbreeding.
The most likely cause of the high number of private alleles in the reintroduced population and the resultant separation between the Argyll and reintroduced populations on the PCA is the composition of the Argyll founders of the captive breeding population. The Argyll component was collected from 19 donor sites of which this study resampled only two, most donor sites no longer hosting the species. Consequently approximately 90% of the potential genetic variation from the Argyll founders is not being represented in this analysis. Despite this, pairwise comparison has shown that Cumbrian populations have a lower degree of differentiation from the Scottish donor populations than from the non-donor populations, suggesting that the Argyll dataset is at least partly representative of the founders used for the reintroduction.
It is also worth recognising that allele frequencies change over time due to natural processes. Approximately 10% of the alleles found in the 1920–1930 Cumbrian samples are not found in any other population, including the 1970–1990 Cumbrian material, their loss probably being due to one or more bottlenecks in the intervening period. Comparable comparisons of historic and current levels of genetic diversity are rare, probably due to the challenges of working with ancient DNA (Nicholls 2005; Bi et al. 2013; Burrell et al. 2015). However a similar loss of genetic diversity and of particular alleles between historic and present populations has been documented in the African lion (Panthera leo) in the Kavango-Zambezi conservation area (Dures et al. 2019).
In the nine generations since the reintroduction was undertaken, some degree of genetic differentiation between the reintroduced populations, akin to that between natural populations, is shown by the pairwise comparison between Cumbrian sites being comparable to those within Argyll. Given that microsatellite markers are selectively neutral (Smee et al. 2013), selection pressures are unlikely to be the cause of population differentiation. Moreover as the populations have only been separate for nine generations it is unlikely that there has been time for sufficient mutations to occur to account for this differentiation (Ellegren 2000). Therefore, the most probable cause is genetic drift. The modelling suggests that the reintroduced populations are behaving genetically as would be expected based on the recorded population sizes and allele frequencies. The simulation shows that drift is sufficient to generate differentiation of this magnitude in a short timeframe. The FST increases with time after reintroduction (Fig. 5a), demonstrating that drift has an ongoing role in shaping genetic structure rather than the initial founder effects that create the differentiation. Genetic drift has the greatest effect in small populations (Willi et al. 2007; Whitlock 2010). Site M would be particularly prone to drift since in annual surveys following the initial reintroduction Site M had the lowest web count compared to the other three sites (Porter and Ellis 2011; CMFAG, pers. comm.). This is likely to account for the highest pairwise scores between Site M and Site E and Site F. This is also reflected in the results from the simulation, where the site was consistently the most distinct from other populations after only one generation of breeding post-reintroduction. The lack of differentiation between Site M and Site B reflects the greater variation in Site B. Differentiation may reasonably be expected to occur in the future.
Similar population differentiation has been seen in the reintroduced populations of the large blue butterfly (Phenargis arion) in the UK as all populations have similar levels of genetic diversity but differing allele frequencies (Andersen et al. 2014), a probable product of the two decades between the reintroduction and genetic study. On the other hand, a study of two locally reintroduced Maculinea spp. in Netherlands found no significant population differentiation after five generations (Wynhoff 2001). Comparable levels of genetic differentiation between natural and reintroduced populations were observed in the Griffon vulture (Gyps fulvus) after approximately eight generations (Le Gouar et al. 2008) and in the hihi (Notiomystis cincta) four to nine generations after reintroduction (Brekke et al. 2011).
Conclusion and management implications
This study has shown that reintroduction following captive breeding is capable of resulting in self-sustaining populations which behave and respond at a population level in a similar manner to natural populations. The study has further demonstrated that a combination of genetic work, modelling and population monitoring alongside an understanding of the species’ biology can reveal the evolutionary dynamics of the reintroduction and its potential for future success. Such an approach may be valid in other species. The rather radical approach of hybridising the last remaining Cumbrian individuals with Scottish stock has to some degree preserved some of the distinction of the Cumbrian population (Fig. 4).
Based on the findings of this study it is recommend that no further genetic management (e.g., translocations or populations supplementations) of the Cumbrian population is undertaken at this time, none of the reintroduced populations studied are at risk of inbreeding depression and as new colonies are establishing naturally (Phoenix 2018) it is reasonable to assume that gene flow between the reintroduced populations will also naturally establish with time as has been seen following reintroductions in other species (Le Gouar et al. 2008). Such establishment of a natural metapopulation structure was seen following the introduction of the Bog Fritillary (Proclossiana eunomia) to a site in Morvan, France (Nève et al. 1996; Nève et al. 2008). The mark release recapture distances observed by Nève et al. (1996) for the Bog Fritillary are on avergae similar to those observed for the Marsh Fritillary (Zimmermann et al. 2011b) with some longer distance (> 10 km) dispersals seen in the Marsh Fritllary, therefore a similar establishment of a natureal metapopulation struture in Cumbria should be expected to occure with time.
On a broader scale, it is recommended that the managers of other declining populations of the Marsh Fritillary strongly consider adopting this management strategy, and if necessary the captive breeding methodology, used in Cumbria (for details see Porter and Ellis 2011). A captive breeding programme for the Apollo butterfly (Parnassius apollo) in Poland resulted in an increase in wing malformations suspected to be at least in part due to the small founder population of approximately 20 individuals resulting in the increased frequency of deleterious recessive alleles in genes such as wingless, although this has not been confirmed (Pierzynowska et al. 2019). This is similar to what could have occurred in Cumbria had the captive breeding programme been instituted sooner, while multiple sibling larval web remained, and therefore without the clear and obvious need for the additional Scottish founder stock. Where a population is not in immediate danger of extinction, captive breeding may be unnecessary, and a similar genetic rescue could be carried out by releasing late-stage instar larvae, collected from a suitable donor population, at the site which is either known or suspected to have low levels of genetic diversity. A low level of gene flow, represented by a few individuals from external sources breeding successfully each year, is thought to be sufficient to avoid inbreeding depression while still retaining any locally advantageous adaptations (Åkesson et al. 2016; Gustafson et al. 2017). This strategy, including the genetic rescue, should also be considered for other Lepidoptera which have similar life histories and conservation concerns (Frankham 2015; Ralls et al. 2018).
Data availability
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Code availability
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Acknowledgements
The authors are grateful to the members of the Cumbrian Marsh Fritillary Action Group for their support in field work and access to reintroduction sites. We are grateful to curators of Tullie House Museum and the Natural History Museum for access to their collections and to Tim Melling and Peter Summers for allowing sampling of their private collections.
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This work was funded by Edge Hill University as part of an internal PhD Studentship.
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All authors contributed to the study conception and design. Material collection was performed by MD and KP. Laboratory work and data analysis were performed by MD. Computer modelling was performed by CB. The first draft of the manuscript was written by MD and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.
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Davis, M.L., Barker, C., Powell, I. et al. Combining modelling, field data and genetic variation to understand the post-reintroduction population genetics of the Marsh Fritillary butterfly (Euphydryas aurinia). J Insect Conserv 25, 875–886 (2021). https://doi.org/10.1007/s10841-021-00354-3
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DOI: https://doi.org/10.1007/s10841-021-00354-3