Abstract
LPS-induced septic cardiomyopathy has been found to be connected with mitochondrial stress through unknown mechanisms. Mitochondrial fission is an early event in mitochondrial dysfunction. The aim of our study was to determine the role and regulatory mechanism of mitochondrial fission in the progression of LPS-induced septic cardiomyopathy, with a particular focus on Mst1 and F-actin. Our data demonstrated that Mst1 expression was rapidly upregulated in LPS-treated hearts and that increased Mst1 promoted cardiomyocyte death by inducing mitochondrial stress. Mechanistically, elevated expression of Mst1 upregulated Drp1, and the latter initiated mitochondrial fission. Excessive mitochondrial fission caused mitochondrial oxidative injury, mitochondrial membrane potential reduction, mitochondrial proapoptotic element translocation into the cytoplasm/nucleus, mitochondrial energy dysfunction and mitochondrial apoptosis activation. Inhibition of mitochondrial fission sustained mitochondrial function and favored cardiomyocyte survival. Furthermore, we identified F-actin degradation as an apparent downstream event of mitochondrial fission activation in the context of LPS-induced septic cardiomyopathy. Stabilization of F-actin attenuated fission-mediated cardiomyocyte death. Altogether, our results define the Mst1/Drp1/mitochondrial fission/F-actin axis as a new signaling pathway that mediates LPS-related septic cardiomyopathy by inducing mitochondrial stress and cardiomyocyte death. Therefore, Mst1 expression, mitochondrial fission modification and F-actin stabilization may serve as potential therapeutic targets for sepsis-related myocardial injury.
Similar content being viewed by others
References
Abdel-Hamid AAM, Firgany AEL (2018) Favorable outcomes of metformin on coronary microvasculature in experimental diabetic cardiomyopathy. J Mol Histol 49:639–649. https://doi.org/10.1007/s10735-018-9801-4
Abdulmahdi W et al (2017) HMGB1 redox during sepsis. Redox Biol 13:600–607. https://doi.org/10.1016/j.redox.2017.08.001
Abeysuriya RG, Lockley SW, Robinson PA, Postnova S (2018) A unified model of melatonin, 6-sulfatoxymelatonin, and sleep dynamics. J Pineal Res. https://doi.org/10.1111/jpi.12474
Angelova PR et al (2018) Mitochondrial dysfunction in Parkinsonian mesenchymal stem cells impairs differentiation. Redox Biol 14:474–484. https://doi.org/10.1016/j.redox.2017.10.016
Antoniou C, Chatzimichail G, Xenofontos R, Pavlou JJ, Panagiotou E, Christou A, Fotopoulos V (2017) Melatonin systemically ameliorates drought stress-induced damage in Medicago sativa plants by modulating nitro-oxidative homeostasis and proline metabolism. J Pineal Res. https://doi.org/10.1111/jpi.12401
Antunes F, Brito PM (2017) Quantitative biology of hydrogen peroxide signaling. Redox Biol 13:1–7. https://doi.org/10.1016/j.redox.2017.04.039
Areti A, Komirishetty P, Akuthota M, Malik RA, Kumar A (2017) Melatonin prevents mitochondrial dysfunction and promotes neuroprotection by inducing autophagy during oxaliplatin-evoked peripheral neuropathy. J Pineal Res. https://doi.org/10.1111/jpi.12393
Armartmuntree N et al (2018) Prolonged oxidative stress down-regulates Early B cell factor 1 with inhibition of its tumor suppressive function against cholangiocarcinoma genesis. Redox Biol 14:637–644. https://doi.org/10.1016/j.redox.2017.11.011
Ba X, Boldogh I (2018) 8-Oxoguanine DNA glycosylase 1: beyond repair of the oxidatively modified base lesions. Redox Biol 14:669–678. https://doi.org/10.1016/j.redox.2017.11.008
Bi J et al (2018) Irisin alleviates liver ischemia-reperfusion injury by inhibiting excessive mitochondrial fission, promoting mitochondrial biogenesis and decreasing oxidative stress. Redox Biol 20:296–306. https://doi.org/10.1016/j.redox.2018.10.019
Bikfalvi A (2017) History and conceptual developments in vascular biology and angiogenesis research: a personal view. Angiogenesis 20:463–478. https://doi.org/10.1007/s10456-017-9569-2
Blackburn NJR et al (2017) Methylglyoxal-derived advanced glycation end products contribute to negative cardiac remodeling and dysfunction post-myocardial infarction. Basic Res Cardiol. https://doi.org/10.1007/s00395-017-0646-x
Blazquez-Castro A (2017) Direct 1O2 optical excitation: a tool for redox biology. Redox Biol 13:39–59. https://doi.org/10.1016/j.redox.2017.05.011
Boga JA, Caballero B, Potes Y, Perez-Martinez Z, Reiter RJ, Vega-Naredo I, Coto-Montes A (2018) Therapeutic potential of melatonin related to its role as an autophagy regulator: a review. J Pineal Res. https://doi.org/10.1111/jpi.12534
Brazao V et al (2017) Melatonin: antioxidant and modulatory properties in age-related changes during Trypanosoma cruzi infection. J Pineal Res. https://doi.org/10.1111/jpi.12409
Cai H, Wang C (2017) Graphical review: the redox dark side of e-cigarettes; exposure to oxidants and public health concerns. Redox Biol 13:402–406. https://doi.org/10.1016/j.redox.2017.05.013
Cai SY et al (2017) HsfA1a upregulates melatonin biosynthesis to confer cadmium tolerance in tomato plants. J Pineal Res. https://doi.org/10.1111/jpi.12387
Caja S, Enriquez JA (2017) Mitochondria in endothelial cells: sensors and integrators of environmental cues. Redox Biol 12:821–827. https://doi.org/10.1016/j.redox.2017.04.021
Carloni S, Riparini G, Buonocore G, Balduini W (2017) Rapid modulation of the silent information regulator 1 by melatonin after hypoxia-ischemia in the neonatal rat brain. J Pineal Res. https://doi.org/10.1111/jpi.12434
Chandra M et al (2018) Cardiac-specific inactivation of LPP3 in mice leads to myocardial dysfunction and heart failure. Redox Biol 14:261–271. https://doi.org/10.1016/j.redox.2017.09.015
Chen LY et al (2017) Melatonin successfully rescues hippocampal bioenergetics and improves cognitive function following drug intoxication by promoting Nrf2-ARE signaling activity. J Pineal Res. https://doi.org/10.1111/jpi.12417
Chen T et al (2018) Sirt1-Sirt3 axis regulates human blood-brain barrier permeability in response to ischemia. Redox Biol 14:229–236. https://doi.org/10.1016/j.redox.2017.09.016
Choi GH, Lee HY, Back K (2017a) Chloroplast overexpression of rice caffeic acid O-methyltransferase increases melatonin production in chloroplasts via the 5-methoxytryptamine pathway in transgenic rice plants. J Pineal Res. https://doi.org/10.1111/jpi.12412
Choi SI, Lee E, Akuzum B, Jeong JB, Maeng YS, Kim TI, Kim EK (2017b) Melatonin reduces endoplasmic reticulum stress and corneal dystrophy-associated TGFBIp through activation of endoplasmic reticulum-associated protein degradation. J Pineal Res. https://doi.org/10.1111/jpi.12426
Cobley JN, Close GL, Bailey DM, Davison GW (2017) Exercise redox biochemistry: conceptual, methodological and technical recommendations. Redox Biol 12:540–548. https://doi.org/10.1016/j.redox.2017.03.022
Cortese-Krott MM et al (2018) Identification of a soluble guanylate cyclase in RBCs: preserved activity in patients with coronary artery disease. Redox Biol 14:328–337. https://doi.org/10.1016/j.redox.2017.08.020
Ding M et al (2018) Dynamin-related protein 1-mediated mitochondrial fission contributes to post-traumatic cardiac dysfunction in rats and the protective effect of melatonin. J Pineal Res. https://doi.org/10.1111/jpi.12447
Dominguez-Rodriguez A et al (2017) Effect of intravenous and intracoronary melatonin as an adjunct to primary percutaneous coronary intervention for acute ST-elevation myocardial infarction: results of the Melatonin Adjunct in the acute myocaRdial Infarction treated with Angioplasty trial. J Pineal Res. https://doi.org/10.1111/jpi.12374
Ji K et al (2018) Regulation of apoptosis and radiation sensitization in lung cancer cells via the Sirt1/NF-kappaB/Smac pathway. Cell Physiol Biochem 48:304–316. https://doi.org/10.1159/000491730
Jin Q et al (2018) DUSP1 alleviates cardiac ischemia/reperfusion injury by suppressing the Mff-required mitochondrial fission and Bnip3-related mitophagy via the JNK pathways. Redox Biol 14:576–587. https://doi.org/10.1016/j.redox.2017.11.004
Karwi QG, Bice JS, Baxter GF (2017) Pre- and postconditioning the heart with hydrogen sulfide (H2S) against ischemia/reperfusion injury in vivo: a systematic review and meta-analysis. Basic Res Cardiol. https://doi.org/10.1007/s00395-017-0664-8
Kiel AM, Goodwill AG, Noblet JN, Barnard AL, Sassoon DJ, Tune JD (2017) Regulation of myocardial oxygen delivery in response to graded reductions in hematocrit: role of K+ channels. Basic Res Cardiol. https://doi.org/10.1007/s00395-017-0654-x
Kleinbongard P, Skyschally A, Gent S, Pesch M, Heusch G (2017) STAT3 as a common signal of ischemic conditioning: a lesson on “rigor and reproducibility” in preclinical studies on cardioprotection. Basic Res Cardiol. https://doi.org/10.1007/s00395-017-0660-z
Landry NM, Cohen S, Dixon IMC (2017) Periostin in cardiovascular disease and development: a tale of two distinct roles. Basic Res Cardiol. https://doi.org/10.1007/s00395-017-0659-5
Li W et al (2017) Long-term spironolactone treatment reduces coronary TRPC expression, vasoconstriction, and atherosclerosis in metabolic syndrome pigs. Basic Res Cardiol. https://doi.org/10.1007/s00395-017-0643-0
Li H et al (2018a) Mst1 deletion attenuates renal ischaemia-reperfusion injury: the role of microtubule cytoskeleton dynamics, mitochondrial fission and the GSK3beta-p53 signalling pathway. Redox Biol 20:261–274. https://doi.org/10.1016/j.redox.2018.10.012
Li R, Xin T, Li D, Wang C, Zhu H, Zhou H (2018b) Therapeutic effect of Sirtuin 3 on ameliorating nonalcoholic fatty liver disease: the role of the ERK-CREB pathway and Bnip3-mediated mitophagy. Redox Biol 18:229–243. https://doi.org/10.1016/j.redox.2018.07.011
Li Z, Qiu R, Qiu X, Tian T (2018c) EYA4 promotes cell proliferation through downregulation of p27Kip1 in glioma. Cell Physiol Biochem 49:1856–1869. https://doi.org/10.1159/000493631
Liu D, Zeng X, Li X, Mehta JL, Wang X (2017) Role of NLRP3 inflammasome in the pathogenesis of cardiovascular diseases. Basic Res Cardiol. https://doi.org/10.1007/s00395-017-0663-9
Liu B, Xu L, Yu X, Jiao X, Yan J, Li W, Guo M (2018) Genistein inhibited estradiol-induced vascular endothelial cell injury by downregulating the FAK/focal adhesion pathway. Cell Physiol Biochem 49:2277–2292. https://doi.org/10.1159/000493830
Maciel L, de Oliveira DF, da Costa GCV, Bisch PM, Nascimento JHM (2017) Cardioprotection by the transfer of coronary effluent from ischaemic preconditioned rat hearts: identification of cardioprotective humoral factors. Basic Res Cardiol. https://doi.org/10.1007/s00395-017-0641-2
Maneechote C, Palee S, Chattipakorn SC, Chattipakorn N (2017) Roles of mitochondrial dynamics modulators in cardiac ischaemia/reperfusion injury. J Cell Mol Med 21:2643–2653. https://doi.org/10.1111/jcmm.13330
Minton K (2015) Phagocytosis: mitochondria and phagosomes: better together. Nat Rev Immunol. https://doi.org/10.1038/nri3931
Morell M, Burgos JI, Gonano LA, Vila Petroff M (2017) AMPK-dependent nitric oxide release provides contractile support during hyperosmotic stress. Basic Res Cardiol. https://doi.org/10.1007/s00395-017-0665-7
Peterson YK et al (2017) Frizzled-5: a high affinity receptor for secreted frizzled-related protein-2 activation of nuclear factor of activated T-cells c3 signaling to promote angiogenesis. Angiogenesis 20:615–628. https://doi.org/10.1007/s10456-017-9574-5
Pryds K et al (2017) Effect of long-term remote ischemic conditioning in patients with chronic ischemic heart failure. Basic Res Cardiol. https://doi.org/10.1007/s00395-017-0658-6
Reddy KRK et al (2018) Dimethylarginine dimethylaminohydrolase-1 (DDAH1) is frequently upregulated in prostate cancer, and its overexpression conveys tumor growth and angiogenesis by metabolizing asymmetric dimethylarginine (ADMA). Angiogenesis 21:79–94. https://doi.org/10.1007/s10456-017-9587-0
Rossello X, Riquelme JA, He Z, Taferner S, Vanhaesebroeck B, Davidson SM, Yellon DM (2017) The role of PI3Kα isoform in cardioprotection. Basic Res Cardiol. https://doi.org/10.1007/s00395-017-0657-7
Schluter KD, Wolf A, Weber M, Schreckenberg R, Schulz R (2017) Oxidized low-density lipoprotein (oxLDL) affects load-free cell shortening of cardiomyocytes in a proprotein convertase subtilisin/kexin 9 (PCSK9)-dependent way. Basic Res Cardiol. https://doi.org/10.1007/s00395-017-0650-1
Schulz R, Agg B, Ferdinandy P (2017) Survival pathways in cardiac conditioning: individual data vs. meta-analyses. What do we learn? Basic Res Cardiol. https://doi.org/10.1007/s00395-017-0661-y
Wang X, Song Q (2018) Mst1 regulates post-infarction cardiac injury through the JNK-Drp1-mitochondrial fission pathway. Cell Mol Biol Lett. https://doi.org/10.1186/s11658-018-0085-1
Yan H, Qiu C, Sun W, Gu M, Xiao F, Zou J, Zhang L (2018) Yap regulates gastric cancer survival and migration via SIRT1/Mfn2/mitophagy. Oncol Rep 39:1671–1681. https://doi.org/10.3892/or.2018.6252
Yang Y et al (2016) The emerging role of Toll-like receptor 4 in myocardial inflammation. Cell Death Dis. https://doi.org/10.1038/cddis.2016.140
Yu W, Xu M, Zhang T, Zhang Q, Zou C (2018) Mst1 promotes cardiac ischemia-reperfusion injury by inhibiting the ERK-CREB pathway and repressing FUNDC1-mediated mitophagy. J Physiol Sci. https://doi.org/10.1007/s12576-018-0627-3
Zhang W, Liu K, Pei Y, Ma J, Tan J, Zhao J (2018) Mst1 regulates non-small cell lung cancer A549 cell apoptosis by inducing mitochondrial damage via ROCK1/Factin pathways. Int J Oncol. https://doi.org/10.3892/ijo.2018.4586
Zhao H, Pan W, Chen L, Luo Y, Xu R (2018a) Nur77 promotes cerebral ischemia-reperfusion injury via activating INF2-mediated mitochondrial fragmentation. J Mol Histol 49:599–613. https://doi.org/10.1007/s10735-018-9798-8
Zhao Q et al (2018b) Effect of Mst1 on endometriosis apoptosis and migration: role of Drp1-related mitochondrial fission and Parkin-required mitophagy. Cell Physiol Biochem 45:1172–1190. https://doi.org/10.1159/000487450
Zhou H et al (2017) Mff-dependent mitochondrial fission contributes to the pathogenesis of cardiac microvasculature ischemia/reperfusion injury via induction of mROS-mediated cardiolipin oxidation and HK2/VDAC1 disassociation-involved mPTP opening. J Am Heart Assoc. https://doi.org/10.1161/JAHA.116.005328
Zhou H et al (2018a) Effects of melatonin on fatty liver disease: the role of NR4A1/DNA-PKcs/p53 pathway, mitochondrial fission, and mitophagy. J Pineal Res. https://doi.org/10.1111/jpi.12450
Zhou H et al (2018b) NR4A1 aggravates the cardiac microvascular ischemia reperfusion injury through suppressing FUNDC1-mediated mitophagy and promoting Mff-required mitochondrial fission by CK2α. Basic Res Cardiol. https://doi.org/10.1007/s00395-018-0682-1
Zhou H, Shi C, Hu S, Zhu H, Ren J, Chen Y (2018c) BI1 is associated with microvascular protection in cardiac ischemia reperfusion injury via repressing Syk-Nox2-Drp1-mitochondrial fission pathways. Angiogenesis 21:599–615. https://doi.org/10.1007/s10456-018-9611-z
Zhou H, Wang J, Hu S, Zhu H, Toanc S, Ren J (2018d) BI1 alleviates cardiac microvascular ischemia-reperfusion injury via modifying mitochondrial fission and inhibiting XO/ROS/F-actin pathways. J Cell Physiol. https://doi.org/10.1002/jcp.27308
Zhou H, Wang J, Zhu P, Hu S, Ren J (2018e) Ripk3 regulates cardiac microvascular reperfusion injury: the role of IP3R-dependent calcium overload, XO-mediated oxidative stress and F-action/filopodia-based cellular migration. Cell Signal 45:12–22. https://doi.org/10.1016/j.cellsig.2018.01.020
Zhou H, Wang S, Hu S, Chen Y, Ren J (2018f) ER-mitochondria microdomains in cardiac ischemia-reperfusion injury: a fresh perspective. Front Physiol. https://doi.org/10.3389/fphys.2018.00755
Zhou H, Wang S, Zhu P, Hu S, Chen Y, Ren J (2018g) Empagliflozin rescues diabetic myocardial microvascular injury via AMPK-mediated inhibition of mitochondrial fission. Redox Biol 15:335–346. https://doi.org/10.1016/j.redox.2017.12.019
Zhou H, Yue Y, Wang J, Ma Q, Chen Y (2018h) Melatonin therapy for diabetic cardiomyopathy: a mechanism involving Syk-mitochondrial complex I-SERCA pathway. Cell Signal 47:88–100. https://doi.org/10.1016/j.cellsig.2018.03.012
Zhu H et al (2018a) Melatonin protected cardiac microvascular endothelial cells against oxidative stress injury via suppression of IP3R-[Ca2+]c/VDAC-[Ca2+]m axis by activation of MAPK/ERK signaling pathway. Cell Stress Chaperones 23:101–113. https://doi.org/10.1007/s12192-017-0827-4
Zhu P et al (2018b) Ripk3 promotes ER stress-induced necroptosis in cardiac IR injury: a mechanism involving calcium overload/XO/ROS/mPTP pathway. Redox Biol 16:157–168. https://doi.org/10.1016/j.redox.2018.02.019
Acknowledgements
The authors are grateful to the Institute of Basic Medicine Science of Qingdao Municipal Hospital.
Funding
This work was supported by Natural Science Foundation of Fujian (Grant number: (2015) 269) and high-level hospital grants from Fujian Provincial Hospital, Fujian province, China (Grant number: (2017) 510#). The funders had no role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Author information
Authors and Affiliations
Contributions
XS and JL involved in conception and design, performance of experiments, data analysis and interpretation, and manuscript writing; RY and PZ involved in data analysis and interpretation; YZ, JX, KC and ML involved in conception and design, data analysis and interpretation, financial support, and final approval of manuscript.
Corresponding authors
Ethics declarations
Conflict of interest
The authors have declared that they have no conflicts of interest.
Additional information
Publisher’s Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
About this article
Cite this article
Shang, X., Li, J., Yu, R. et al. Sepsis-related myocardial injury is associated with Mst1 upregulation, mitochondrial dysfunction and the Drp1/F-actin signaling pathway. J Mol Hist 50, 91–103 (2019). https://doi.org/10.1007/s10735-018-09809-5
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10735-018-09809-5