Abstract
Sequence-Specific Amplified Polymorphism (S-SAP)-analysis of 131 accessions of different species from five sections of the genus Malus was carried out to study genetic diversity, and to clarify phylogenetic and taxonomic issues. S-SAP-markers, based on long terminal repeat (LTR)-retrotransposons TRIM2 and dem1, were developed, which identified 679 polymorphic fragments in the studied Malus species. S-SAP technique proved to be effective for taxonomic studies in Malus. The obtained results generally support the existing sectional taxonomy in the genus Malus and allowed to determine the taxonomic status of some Russian landraces. The genetic diversity and taxonomic status of the Russian apple Antonovka landraces, widely used in breeding programs for their increased adaptation to abiotic stress and scab resistance, were clarified. All of them belong to M. domestica, section Malus. However, Antonovka Olginskaya might have a hybrid origin with some contribution from Gymnomeles species according to PCO analysis data. The taxonomic status was resolved for another Russian landrace, Yakutskaya, which exhibits increased winter hardiness and drought resistance; it belongs to section Gymnomeles, with a high resemblance to M. baccata.
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Acknowledgments
This work was supported by the Russian Science Foundation project No 14-16-00121. The authors would like to thank the Vavilov Research Institute of Plant Industry (VIR) (St. Petersburg, Russia), the Michurin All-Russian Research Institute of Genetics and Breeding of Fruit Crops (Michurinsk, Russia), and the State Botanical Garden of the Russian Academy of Sciences (Moscow, Russia) for the plant material (apple leaves and buds) provided for this study.
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Savelyeva, E., Kalegina, A., Boris, K. et al. Retrotransposon-based sequence-specific amplified polymorphism markers for the analysis of genetic diversity and phylogeny in Malus Mill. (Rosaceae). Genet Resour Crop Evol 64, 1499–1511 (2017). https://doi.org/10.1007/s10722-016-0449-1
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DOI: https://doi.org/10.1007/s10722-016-0449-1