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A detailed linkage map of lettuce based on SSAP, AFLP and NBS markers

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Abstract

Molecular markers based upon a novel lettuce LTR retrotransposon and the nucleotide binding site-leucine-rich repeat (NBS-LRR) family of disease resistance-associated genes have been combined with AFLP markers to generate a 458 locus genetic linkage map for lettuce. A total of 187 retrotransposon-specific SSAP markers, 29 NBS-LRR markers and 242 AFLP markers were mapped in an F2 population, derived from an interspecific cross between a Lactuca sativa cultivar commonly used in Europe and a wild Lactuca serriola isolate from Northern Europe. The cross has been designed to aid efforts to assess gene flow from cultivated lettuce into the wild in the perspective of genetic modification biosafety. The markers were mapped in nine major and one minor linkage groups spanning 1,266.1 cM, with an average distance of 2.8 cM between adjacent mapped markers. The markers are well distributed throughout the lettuce genome, with limited clustering of different marker types. Seventy-seven of the AFLP markers have been mapped previously and cross-comparison shows that the map from this study corresponds well with the previous linkage map.

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Acknowledgements

This work was supported by Grant 01657 (ANGEL) from the European Commission under the Frameworks VI Programme. We are very grateful to Richard Michelmore, Maria Truco and Alex Kozic who generously provided us with lettuce EST data filtered for transposon sequences prior to publication, together with much helpful advice on linkage mapping in lettuce. We are also thankful for many interesting and fruitful discussions from the other members of the ANGEL partnership—Rikke Bagger Jørgensen, François Felber, Jeroen Rouppe Van der Voort, Gerard Ostermeier and Massimo Vischi.

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Authors and Affiliations

  1. Plant Research Unit, University of Dundee at SCRI, Invergowrie, Dundee, DD2 5DA, UK

    Naeem H. Syed & Andrew J. Flavell

  2. Keygene N.V. Applied Research, 216, 6700, Wageningen, AE, The Netherlands

    Anker P. Sørensen & Rudie Antonise

  3. Plant Research International B.V., Wageningen University and Research Centre, 16, 6700, Wageningen, AA, The Netherlands

    Clemens van de Wiel, C. Gerard van der Linden & Wendy van ‘t Westende

  4. Institute for Biodiversity and Ecosystem Dynamics, University of Amsterdam, Kruislaan 318, 1098, Amsterdam, SM, The Netherlands

    Danny A. P. Hooftman & Hans C. M. den Nijs

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  1. Naeem H. Syed
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  2. Anker P. Sørensen
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  3. Rudie Antonise
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  4. Clemens van de Wiel
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  5. C. Gerard van der Linden
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  6. Wendy van ‘t Westende
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  7. Danny A. P. Hooftman
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  8. Hans C. M. den Nijs
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  9. Andrew J. Flavell
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Correspondence to Andrew J. Flavell.

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Communicated by J. S. Heslop-Harrison

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Syed, N.H., Sørensen, A.P., Antonise, R. et al. A detailed linkage map of lettuce based on SSAP, AFLP and NBS markers. Theor Appl Genet 112, 517–527 (2006). https://doi.org/10.1007/s00122-005-0155-4

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  • DOI: https://doi.org/10.1007/s00122-005-0155-4

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