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DNA marker analysis for pyramided of Fusarium head blight (FHB) resistance QTLs from different germplasm

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Abstract

A pyramided FHB resistance line of wheat (WSY) was previously developed from three FHB resistant cultivars (Sumai 3, Wangshuibai, and Nobeokabouzu) in the Jiangsu Academy of Agricultural Sciences, China. In the present study, we analyzed the genetic relationship between WSY and the three parental cultivars using DNA markers in order to clarify how many and which resistance genes had accumulated in WSY. We analyzed 282 DNA markers from the 21 wheat chromosomes. WSY was found to include different chromosome regions that harbored putative FHB QTLs of the three parental germplasm. Haplotypes of DNA markers on these QTL regions revealed that the 1BL, 2BL, 5AS, and 7AL QTL regions were from Sumai 3, the 2AS, 2DS, 3AS, and 6BS QTL regions were from Wangshuibai, and the 3BS QTL region was from Nobeokabouzu. This study showed that different resistance genes from the different resistant germplasm had indeed accumulated in WSY. WSY is a potential resistant resource for FHB resistance in wheat breeding programs.

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Acknowledgements

This research was partially funded by the Asia-Biotechnology fellowship program of the Japan International Research Center for Agricultural Sciences (JIRCAS), Japan and the Science foundation (BK2004171) and Talent foundation (2004, type A) projects of Jiangsu province, China.

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Authors and Affiliations

  1. Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China

    J. R. Shi, H. Y. Yang & Q. X. Lu

  2. Biological Resources Division, Japan International Research Center for Agricultural Sciences (JIRCAS), 1-1 Ohwashi, Tsukuba, Ibaraki, 305-8686, Japan

    D. H. Xu & T. Ban

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  1. J. R. Shi
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  2. D. H. Xu
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  3. H. Y. Yang
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  4. Q. X. Lu
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  5. T. Ban
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Correspondence to D. H. Xu.

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Shi, J.R., Xu, D.H., Yang, H.Y. et al. DNA marker analysis for pyramided of Fusarium head blight (FHB) resistance QTLs from different germplasm. Genetica 133, 77–84 (2008). https://doi.org/10.1007/s10709-007-9186-x

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  • DOI: https://doi.org/10.1007/s10709-007-9186-x

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