Abstract
Pod number per plant (PN) is an important yield component trait in peanut. This study used a recombinant inbred line (RIL) population consisting of 181 lines to assess this trait across six environments. Continuous distributions and transgressive segregations were observed in all measured environments. The broad-sense heritability of PN was estimated to be 0.84. Quantitative trait locus (QTL) mapping for each environment identified a total of 11 additive QTLs with the phenotypic variation explained (PVE) of 5.12–24.91%. Multi-environment QTL analysis demonstrated that the interaction between additive loci and environments accounted for 0.05–1.93% PVEs. Two consensus QTLs (cqPN6 and cqPN7) on chromosomes 6 and 7 were detected in at least two environments. CqPN7 had a greater contribution to PN than cqPN6. Gene mining of cqPN6 and cqPN7 regions revealed 25 and 42 gene models, respectively. In addition, a total of 26 pairs of epistatic interactions involving 43 genetic loci were detected, with PVEs by epistasis (AA) and epistasis × environment (AAE) interactions up to 2.00% and 0.91%, respectively. These results provided genetic information that can be applied to marker-assisted selection of PN in peanut.
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Funding
This work was supported by the National Natural Science Foundation of China (32001584, 32201876), the Natural Science Foundation of Shandong Province (ZR202111290099), the Major Science and Technology Program of Xinjiang Uygur Autonomous Region (2022A02008-3), the Breeding Project from Department Science & Technology of Shandong Province (2020LZGC001), the Agricultural Scientific and the Technological Innovation Project of Shandong Academy of Agricultural Sciences (CXGC2022A21, CXGC2022A03) and the Qingdao People’s Livelihood Science and Technology Program (20-3-4-26-nsh).
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SZ and JC conceived and designed the research. XH and HM developed the RIL population and conducted field management. SZ and FW collected the phenotypic data. WY, FC and SX performed statistical analyses. SZ, JG and KZ performed QTL analysis and functional annotation. SZ, YC, JC and HY wrote and revised the manuscript. All the authors read and approved the final manuscript for submission.
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Zhang, S., Hu, X., Wang, F. et al. Genetic dissection of additive and epistatic quantitative trait loci controlling pod number per plant in peanut (Arachis hypogaea L.). Euphytica 219, 31 (2023). https://doi.org/10.1007/s10681-023-03162-9
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DOI: https://doi.org/10.1007/s10681-023-03162-9