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Assessing the agronomic potential of linseed genotypes by multivariate analyses and association mapping of agronomic traits

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Abstract

High prices of fish oil make linseed attractive for aquaculture and animal feed. To ensure a constant supply of linseed, the development of stable cultivars is of strategic importance. In this study, 35 linseed genotypes were evaluated in five Chilean environments (E) from 2009 to 2012. The additive main effect and multiplicative interaction analysis (AMMI), genotype (G) plus genotype by environment (GE) interaction (GGE) biplot analysis and three stability parameters were tested with the aim of identifying adapted genotypes for the development of linseed cultivars. An association mapping (AM) analysis was also conducted for four agronomic traits and the stability of the associated markers was evaluated using the QQE (QTL main effect and QTL by environment interaction) approach. Combined analysis of variance for yield, seeds per boll (SPB), plant height (PH) and days to flowering (DTF) were significant for G, E and GE (P < 0.001). The combined stability analysis identified some Canadian, Argentinean and Chilean accessions to be the best adapted and highest yielding genotypes. Coancestry analysis indicated that crossing Canadian and Chilean genotypes could maximize transgressive segregation for yield. Significant associations for DTF, PH and SPB explained up to 59 % of the phenotypic variation for these traits. The QQE and AM analyses were consistent in identifying marker LGM27B as the most stable and significant across all environments with the largest effect in reducing DTF. The combined application of the stability, AM and QQE analyses could accelerate the development of marketable linseed cultivars adapted to Southern Chile.

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Acknowledgments

The authors are grateful to Héctor Pauchard for supplying the meteorological data and Dana Kowal for the help in the preparation of figures. We acknowledge INIA and Monica Gebert for their support providing experimental fields. This work was supported by the Agriaquaculture Nutritional Genomic Center (CGNA). Braulio Soto-Cerda was supported by Becas Chile—Comisión Nacional de Investigación Científica y Tecnológica (CONICYT).

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Authors and Affiliations

  1. Cereal Research Centre, Agriculture and Agri-Food Canada, 195 Dafoe Rd, Winnipeg, MB, R3T 2M9, Canada

    Braulio J. Soto-Cerda & Sylvie Cloutier

  2. Genomics and Bioinformatics Unit, Agriaquaculture Nutritional Genomic Center (CGNA), INIA, Km 10 Camino Cajón-Vilcún, Temuco, Chile

    Braulio J. Soto-Cerda, Fernando Westermeyer & Federico Iñiguez-Luy

  3. Centro de Biología Gran Concepción, Facultad de Ciencias Biológicas, Universidad Andrés Bello, Sede Concepción, Autopista Concepción-Talcahuano, 7100, Talcahuano, Chile

    Gastón Muñoz

  4. Instituto de Investigaciones Agropecuarias, INIA, Carillanca, Camino Cajón-Vilcún Km 10, PO Box-58D, Temuco, Chile

    Adolfo Montenegro

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  1. Braulio J. Soto-Cerda
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  2. Fernando Westermeyer
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  3. Federico Iñiguez-Luy
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  4. Gastón Muñoz
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  5. Adolfo Montenegro
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  6. Sylvie Cloutier
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Correspondence to Sylvie Cloutier.

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Soto-Cerda, B.J., Westermeyer, F., Iñiguez-Luy, F. et al. Assessing the agronomic potential of linseed genotypes by multivariate analyses and association mapping of agronomic traits. Euphytica 196, 35–49 (2014). https://doi.org/10.1007/s10681-013-1012-1

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