Abstract
Safflower (Carthamus tinctorius L.) DNA marker resources are currently very limited. The objective of this study was to determine the feasibility of transferring non-genic microsatellite (SSR) markers and gene-based markers, including intron fragment length polymorphism (IFLP) and resistance gene candidates (RGC)-based markers from sunflower (Helianthus annuus L.) to safflower, both species belonging to the Asteraceae family. Cross-species amplification of 119 non-genic SSRs, 48 IFLPs, and 19 RGC-based sunflower markers in 22 lines and germplasm accessions of safflower was evaluated. Additionally, 69 EST-SSR markers previously reported to amplify in safflower were tested. The results showed that 17.6% of the non-genic SSR, 56.2% of the IFLP, and 73.7% of the RGC-based markers were transferable to safflower. The percentage of transferable markers showing polymorphic loci was 66.6% for non-genic SSR, 70.6% for EST-SSR, 55.5% for IFLP, and 71.4% for RGC-based markers. The highest polymorphism levels were found for non-genic SSR. The average number of alleles per polymorphic locus and mean heterozygosity values were 2.9 and 0.46, respectively, for non-genic SSR, 2.2 and 0.35 for EST-SSR, 2.1 and 0.24 for IFLP, and 2.0 and 0.34 for RGC-based markers. The results of this study revealed a low rate of transferability for non-genic SSR sunflower markers and a better rate of transferability for IFLP and RGC-based markers. Transferable genic and non-genic sunflower markers can have utility for trait and comparative mapping studies in safflower.
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Acknowledgments
The authors would like to thank Dr. Alberto J. Leon (Advanta Semillas, Balcarce, Buenos Aires, Argentina) and Dr. Steven J. Knapp (The University of Georgia, Athens, USA) for kindly providing IFLP and RGC markers, respectively. The research was funded by the Spanish Ministry of Science and Innovation, research project AGL-2007-62834.
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García-Moreno, M.J., Velasco, L. & Pérez-Vich, B. Transferability of non-genic microsatellite and gene-based sunflower markers to safflower. Euphytica 175, 145–150 (2010). https://doi.org/10.1007/s10681-010-0139-6
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DOI: https://doi.org/10.1007/s10681-010-0139-6