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Overexpression of ERF96, a small ethylene response factor gene, enhances salt tolerance in Arabidopsis

  • Original paper
  • Published:
Biologia Plantarum

Abstract

Salt stress is one of the abiotic stresses limiting the yield of crops worldwide. However, the molecular mechanisms underlying the regulation of plant response to salt stress are not completely elucidated. Ethylene response factors (ERFs) are a subfamily of the AP2 (APETALA2)/ERF transcription factor family that regulates multiple aspects of plant growth and development, and plant responses to biotic and abiotic stresses. ERF96 is one of the small ERFs that is involved in plant defense response and abscisic acid signaling in Arabidopsis. By using real time quantitative PCR, we found that the expression of ERF96 in the wild type Arabidopsis thaliana (cv. Col-0) seedlings was induced by NaCl treatment. The transgenic plants overexpressing ERF96 were more tolerant to salt stress in terms of NaCl inhibited seed germination, early seedling development, and fresh mass. Consistent with these observations, elevated expressions of some NaCl-responsive genes including responsive drought 29 (RD29A), Δ1-pyrroline-5-carboxylate synthetase (P5CS), cold responsive 15A (COR15A), and kinase 1 (KIN1) were observed in the transgenic plants in the presence of NaCl. We also found that the Na+ and K+ content and expressions of genes related to Na+/K+ homeostasis including stelar K+ outward rectifier (SKOR) and potassium transport 2/3 (AKT2/3) were altered in the ERF96 transgenic plants in response to NaCl treatment. Taken together, these results showed that overexpression of ERF96 enhanced plant tolerance to salt stress, indicating that ERF96 is a positive regulator of salt tolerance in Arabidopsis.

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Abbreviations

ABA:

avscisic acid

AKT2/3:

potassium transporter 2/3

AP2:

APETALA2

AsA:

ascorbic acid

COR15A:

coldresponsive 15A

ERF:

ethylene response factor

KIN1:

kinase 1

MS:

Murashige and Skoog

P5CS:

Δ1-pyrroline-5-carboxylate synthetase

RAV:

related to ABI3/VP1

RD29A:

responsive drought 29A

SKOR:

stelar K+ outward rectifier

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  1. Key Laboratory of Molecular Epigenetics of MOE and Institute of Genetics & Cytology, Northeast Normal University, Changchun, 130024, P.R. China

    X. Wang, C. Hou, K. Zheng, Q. Li, S. Chen & S. Wang

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  1. X. Wang
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  2. C. Hou
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  3. K. Zheng
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  4. Q. Li
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  6. S. Wang
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Correspondence to S. Wang.

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Acknowledgements: We thank all the laboratory members for their helpful discussion. This work was supported by the National Key Program for Research and Development (2016YFD0101900). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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Wang, X., Hou, C., Zheng, K. et al. Overexpression of ERF96, a small ethylene response factor gene, enhances salt tolerance in Arabidopsis . Biol Plant 61, 693–701 (2017). https://doi.org/10.1007/s10535-017-0734-7

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