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Functional characterization of the antioxidant enzymes in rice plants exposed to salinity stress

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  • Published:
Biologia Plantarum

Abstract

The objective of this study was to relate the activation of enzymatic antioxidant system to the production of reactive oxygen species induced by salt stress. Rice (Oryza sativa L.) genotypes BRS Bojuru and BRS Pampa, tolerant and sensitive to salinity, respectively, were subjected to 150 mM NaCl for 0, 6, 24, 48, and 72 h. A significant increase of superoxide anion and H2O2 and a decrease in malondialdehyde (MDA) content were observed in the tolerant genotype, whereas in the sensitive genotype, there was no change in superoxide anion content, reduced H2O2 content, and increased MDA content. The superoxide dismutase (SOD) activity increased significantly in both genotypes, and increases in amounts of transcript were observed for OsSOD3Cu/Zn and OsSODA1-Mn in the tolerant genotype and for OsSOD4-Cu/Zn, OsSOD3-Cu/Zn, OsSODCc1-Cu/Zn, OsSOD-Fe, and OsSODA1-Mn in the sensitive genotype. The activities of catalase (CAT), ascorbate peroxidase (APX), and glutathione reductase (GR) were not significantly and consistently changed, but OsCATA, OsAPX2 and OsGR1 were induced in both genotypes. OsCATB transcription was increased in the tolerant genotype and OsCATC and OsAPX3 in the sensitive genotype under salinity. It is concluded that OsAPX3, OsGR2, OsGR3, and OsSOD3-Cu/Zn genes are the most suitable to distinguish tolerant from sensitive genotypes under salt stress.

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Abbreviations

APX:

ascorbate peroxidase

CAT:

catalase

GR:

glutathione reductase

MDA:

malondialdehyde

ROS:

reactive oxygen species

SOD:

superoxide dismutase

TBA:

thiobarbituric acid

TCA:

trichloroacetic acid

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Authors and Affiliations

  1. Department of Plant Physiology, Federal University of Pelotas, Pelotas, 96160-000, RS, Brazil

    I. L. Vighi, L. C. Benitez, M. N. Amaral, G. P. Moraes, P. A. Auler, G. S. Rodrigues, S. Deuner & E. J. B. Braga

  2. Plant Breeding Center, Federal University of Pelotas, Pelotas, 96160-000, RS, Brazil

    L. C. Maia

Authors
  1. I. L. Vighi
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  2. L. C. Benitez
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  3. M. N. Amaral
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  4. G. P. Moraes
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  5. P. A. Auler
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  6. G. S. Rodrigues
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  7. S. Deuner
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  8. L. C. Maia
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  9. E. J. B. Braga
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Correspondence to I. L. Vighi.

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Acknowledgements: Research supported by the following Brazilian funding agencies: Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), and Fundação de Amparo à Pesquisa do Rio Grande do Sul (FAPERGS).

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Vighi, I.L., Benitez, L.C., Amaral, M.N. et al. Functional characterization of the antioxidant enzymes in rice plants exposed to salinity stress. Biol Plant 61, 540–550 (2017). https://doi.org/10.1007/s10535-017-0727-6

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  • DOI: https://doi.org/10.1007/s10535-017-0727-6

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