Abstract
A halophilic archaeal strain, SKJ47T, was isolated from a commercial preparation of the brown alga Laminaria produced at Dalian, Liaoning Province, China. Cells of the strain were observed to be short rods, stain Gram-negative, and to form red-pigmented colonies on solid media. Strain SKJ47T was found to be able to grow at 20–50 °C (optimum 37 °C), at 0.9–4.8 M NaCl (optimum 2.6–3.1 M), at pH 6.0–9.5 (optimum pH 7.0). The cells lysed in distilled water and the minimal NaCl concentration to prevent cell-lysis was found to be 5 % (w/v). The major polar lipids of the strain were identified as phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, phosphatidylglycerol sulfate and two glycolipids chromatographically identical to those of Halopenitus persicus IBRC 10041T. The 16S rRNA gene and rpoB′ gene of strain SKJ47T were found to be phylogenetically related to the corresponding genes of Halopenitus malekzadehii IBRC-M 10418T (96.3 and 91.9 % nucleotide identity, respectively) and Hpt. persicus IBRC 10041T (96.2 and 93.8 %). The DNA G+C content of strain SKJ47T was determined to be 65.0 mol%. The phenotypic, chemotaxonomic and phylogenetic properties suggested that strain SKJ47T (=CGMCC 1.12229T = JCM 18641T) represents a new species of the genus Halopenitus, for which the name Halopenitus salinus sp. nov. is proposed.
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Acknowledgments
This work was supported by the National Natural Science Foundation of China (No. 31370054), the grant from China Ocean Mineral Resources R & D Association (COMRA) Special Foundation (DY125-15-R-03), the Qinglan Project of Jiangsu Province and a project funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD). We are grateful to Dr. M. A. Amoozegar for kindly providing strains Halopenitus persicus IBRC 10041T and Halopenitus malekzadehii IBRC-M 10418T.
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Phase-contrast micrograph of strain SKJ47T, thin-layer chromatograms of strain SKJ47T and related members are available as supplementary materials.
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Han, D., Cui, HL. & Li, ZR. Halopenitus salinus sp. nov., isolated from the brine of salted brown alga Laminaria . Antonie van Leeuwenhoek 106, 743–749 (2014). https://doi.org/10.1007/s10482-014-0244-y
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DOI: https://doi.org/10.1007/s10482-014-0244-y