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Migration of biological control agent Rhizobium vitis strain ARK-1 in grapevine stems and inhibition of galls caused by tumorigenic strain of R. vitis

  • Bacterial and Phytoplasma Diseases
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Abstract

Nonpathogenic strain ARK-1 of Rhizobium vitis has antagonistic activity against a tumorigenic (Ti) strain of R. vitis and works as a biocontrol agent, but the temporal dynamics of the bacterial colonization in plants needs to be understood. Here we showed that the incidence of galls induced by Ti strain was reduced to about 60% when both upper and lower positions on the grapevine stem were inoculated with ARK-1 3 cm from the site that was inoculated with Ti 5 days later. A bacterial cell detection assay in the grapevine tissue revealed that ARK-1 moved at least 3 cm in 5 days.

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Acknowledgements

This research was supported by KAKENHI Grant 17H03778 and 20K20572 from the Ministry of Education, Culture, Sports, Science and Technology of Japan to A. Kawaguchi and Y. Noutoshi. We are grateful to Dr. K. Inoue (Research Institute for Agriculture, Okayama Prefectural Technology Center for Agriculture, Forestry and Fisheries) who gave us much useful advice.

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Authors and Affiliations

  1. Western Region Agricultural Research Center (WARC), National Agriculture and Food Research Organization (NARO), 6-12-1 Nishifukatsu-cho, Fukuyama, Hiroshima, 721-8514, Japan

    Akira Kawaguchi

  2. Graduate School of Environmental and Life Science, Okayama University, 1-1-1 Tsushima-naka, Kita-ku, Okayama, 700-8530, Japan

    Yoshiteru Noutoshi

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  1. Akira Kawaguchi
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  2. Yoshiteru Noutoshi
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Correspondence to Akira Kawaguchi.

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10327_2021_1043_MOESM3_ESM.pptx

Supplementary Fig. S1. The four treatments (i ii, iii, and iv) used to investigate gall suppression activity of ARK-1 at a distance from the inoculation site. (i) Sterile distilled water was pipetted into both upper and lower sites, and Ti strain was pipetted into the middle site. (ii) ARK-1 was pipetted into both upper and lower sites, and Ti strain was pipetted into the middle site. (iii) Sterile distilled water was pipetted into the upper and lower sites, and 5 days later (5 dai), Ti strain was pipetted into the middle site. (iv) ARK-1 was pipetted into the upper and lower sites, and 5 days later, Ti strain was pipetted into the middle site. Galls were counted and measured 5 months later. (PPTX 82 KB)

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Kawaguchi, A., Noutoshi, Y. Migration of biological control agent Rhizobium vitis strain ARK-1 in grapevine stems and inhibition of galls caused by tumorigenic strain of R. vitis. J Gen Plant Pathol 88, 63–68 (2022). https://doi.org/10.1007/s10327-021-01043-4

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