Abstract
Agrocin-producing pathogenic and nonpathogenic biotype-3 strains ofAgrobacterium tumefaciens were isolated from grapevine gall tissue. In vitro activity of the nonpathogenic agrocin producers was restricted to biotype-3 pathogens used. Pathogenic agrocin producers were active in vitro against biotype-3 agrocin-producing nonpathogens, non-agrocin-producing pathogens, and biotype-1 strains when cultivated on a modified Stonier's medium; on a medium designated AB, two strains stested showed no activity against agrocin-producing nonpathogens, but agrocin of one of these strains was active against other agrocin-producing pathogens. In a greenhouse experiment a marked tendency toward decreased gall formation by biotype-3 pathogens on grapevines was obtained when biotype-3 pathogens and nonpathogenic biotype-3 agrocin producers were applied to wounds simultaneously. In this experiment, agrocin-producting pathogens tended to be more virulent than non-agrocin-producing pathogens.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Literature Cited
Allen ON (1959) Experiments in soil bacteriology. Minneapolis: Burgess
Bernaerts MJ, De Ley J (1963) A biochemical test for crown gall bacteria. Nature (Lond) 197:406–907
Brisbane PG, Kerr A (1983) Selective media for the three biovars ofAgrobacterium. J. Appl Bacteriol 54:425–431
Clarck AG (1969) A selective medium for the isolation ofAgrobacterium species. J Appl Bacteriol 32:348–351
Cooksey DA, Moore LW (1980) Biological control of crown gall with fungal and bacterial antagonists. Phytopathology 70:506–509
Cooksey DA, Moore LW (1982) Biological control of crown gall with an agrocin mutant ofAgrobacterium radiobacter. Phytopathology 72:919–921
Ellis JG, Kerr A, Van Montagu M, Schell J (1979)Agrobacterium: genetic studies on agrocin 84 production and the biological control of crown gall. Physiol Plant Pathol 15:311–319.
Engler G, Holster M Van Montagu M, Schell J, Hernalsteens JP, Schilperoort R (1975) Agrocin 84 sensitivity: a plasmid determined property ofAgrobacterium tumefaciens. Mol Gen Genet 138:345–349
Hendson M, Askjaer L, Thomson JA, Van Montagu M (1983) Broad-host-range agrocin ofAgrobacterium tumefaciens. Appl Environ Microbiol 45:1526–1532
Htay K, Kerr A (1974) Biological control of crown gall: seed and root inoculation. J Appl Bacteriol 37:525–530
Kechru RB, Singh RN, Yadav IS (1972) Physiological studies on dormancy in grape seeds (Vitis vinifera var. Black Muscat). II. On the effect of growth substances, low chilling temperature and subjection of the seeds to running water. Vitis 11:289–295
Kerr A (1972) Biological control of crown gall: seed inoculation. J Appl Bacteriol 35:493–497
Kerr A (1974) Soil microbiological studies onAgrobacterium radiobacter and biological control of crown gall. Soil Sci 118:168–172
Kerr A (1980) Biological control of crown gall through production of agrocin 84. Plant Dis 64:25–40
Kerr A, Htay K (1974) Biological control of crown gall through bacteriocin production. Physiol Plant Pathol 4:37–44
Kerr A, Panagopoulos CG (1977) Biotypes ofAgrobacterium radiobacter var.tumefaciens and their biological control. Phytopathol Z 90:172–179
Kerr A, Roberts WP (1976)Agrobacterium: correaltions between transfer of pathogenicity, octopine and nopaline metabolism and bacteriocin 84 sensitivity. Physiol Plant Pathol 9:205–211
Lippincott BB, Lippincott JA (1969) Bacterial attachment to a specific wound site as an essential stage in tumor initiation byAgrobacterium tumefaciens. J Bacteriol 97:620–628
Loubser JT (1978) Identification ofAgrobacterium tumefaciens biotype 3 on grapevine in South Africa. Plant Dis Rep 62:730–731
McCardell BA, Pootjes CF (1976) Chemical nature of agrocin 84 and its effect on a virulent strain ofAgrobacterium tumefaciens. Antimicrob Agents Chemother 10:498–502
Moore LW (1977) Prevention of crown gall onPrunus roots by bacterial antagonists. Phytopathology 67:139–144
Moore LW, Warren G (1979)Agrobacterium radiobacter strain 84 and biological control of crown gall. Annu Rev Phytopathol 17:163–179
New PB, Kerr A (1971) A selective medium forAgrobacterium radiobacter biotype 2. J Appl Bacteriol 34:233–236
New PB, Kerr A (1972) Biological control of crown gall: field measurements and glasshouse experiments. J Appl Bacteriol 35:279–287
Panagopoulos CG, Psallidas PG, Alivizatos AS (1978) Studies on biotype 3 ofAgrobacterium radiobacter var.tumefaciens. In: Proceedings of the fourth international conference on pathological bacteria. Angers, France: Gilbert, pp 221–228
Schroth MN, Thompson JR, Hildebrandt DC (1965) Isolation ofAgrobacterium tumefaciens—A. radiobacter group from soil. Phytopathology 55:645–647
Stonier T (1960)Agrobacterium tumefaciens Conn. II. Production of an antibiotic substance. J Bacteriol 79:889–898
Vincent JM (1970) A manual for the practical study of rootnodule bacteria, 1st edn. IBP handbook 15, Oxford: Blackwell
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Staphorst, J.L., van Zyl, F.G.H., Strijdom, B.W. et al. Agrocin-producing pathogenic and nonpathogenic biotype-3 strains ofAgrobacterium tumefaciens active against biotype-3 pathogens. Current Microbiology 12, 45–52 (1985). https://doi.org/10.1007/BF01567753
Issue Date:
DOI: https://doi.org/10.1007/BF01567753