Skip to main content
SpringerLink
Log in

Inhibition of crown gall formation by Agrobacterium radiobacter biovar 3 strains isolated from grapevine

  • BACTERIAL AND PHYTOPLASMA DISEASES
  • Published:
Journal of General Plant Pathology Aims and scope Submit manuscript

Abstract

Graft unions of nursery stock of grapevine (Vitis vinifera L.) collected in Japan yielded pathogenic and nonpathogenic strains of Agrobacterium. On the basis of classical diagnostic tests, a sequence analysis, and a multiplex polymerase chain reaction method previously reported, the pathogenic strain was identified as Agrobacterium tumefaciens biovar 3, whereas the nonpathogenic strains were assigned to Agrobacterium radiobacter biovar 3. Stems of tomato (Lycopersicon esculentum Mill.) seedlings were inoculated with both A. tumefaciens biovar 3 strain G-Ag-27 as a pathogen and one of the control strains isolated from grapevine or A. radiobacter biovar 2 strain K84 as competitors to assay the suppression of gall formation caused by the pathogen. In a test with a 1 : 1 pathogen/nonpathogen cell ratio, all A. radiobacter biovar 3 strains reduced gall incidence and size compared to that of the positive control inoculated only with the pathogen. Strain VAR03-1 was especially effective in reducing the incidence of gall formation on grapevine and reduced gall size by 84%–100% of those on the positive control. Many tested nonpathogenic biovar 3 strains were bacteriocinogenic, causing an inhibition zone against A. tumefaciens biovar 3 strains on YMA medium. Strain VAR03-1 was the most effective against indicator strains and appears to be a promising agent for controlling crown gall of grapevine.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • H Bouzar WS Chilton X Nesme Y Dessaux V Vaudequin A Petit JB Jones NC Hodge (1995) ArticleTitleA new Agrobacterium strain isolated from aerial tumors on Ficus benjamina L Appl Environ Microbiol 61 65–73 Occurrence Handle7887626 Occurrence Handle1:CAS:528:DyaK2MXivValur8%3D

    PubMed  CAS  Google Scholar 

  • TJ Burr BH Katz (1984) ArticleTitleGrapevine cuttings as potential sites of survival and means of dissemination of Agrobacterium tumefaciens Plant Dis 68 976–978

    Google Scholar 

  • TJ Burr L Otten (1999) ArticleTitleCrown gall of grape: biology and disease management Annu Rev Phytopathol 37 53–80 Occurrence Handle11701817 Occurrence Handle1:CAS:528:DyaK1MXnt1Wntrs%3D Occurrence Handle10.1146/annurev.phyto.37.1.53

    Article  PubMed  CAS  Google Scholar 

  • TJ Burr CL Reid (1993) ArticleTitleBiological control of grape crown gall with nontumorigenic Agrobacterium vitis F2/5 Am J Enol Vitic 45 213–219

    Google Scholar 

  • TJ Burr AL Bishop BH Katz LM Blanchard C Bazzi (1987) ArticleTitleA root-specific decay of grapevine caused by Agrobacterium tumefaciens and A. radiobacter biovar 3 Phytopathology 77 1424–1427

    Google Scholar 

  • TJ Burr CL Reid E Taglicti C Bazzi S Süle (1997) ArticleTitleBiological control of grape crown gall by strain F2/5 is not associated with agrocin production or competition for attachment site on grape cells Phytopathology 87 706–711 Occurrence Handle18945092 Occurrence Handle1:STN:280:DC%2BD1cjjvVeruw%3D%3D

    PubMed  CAS  Google Scholar 

  • TJ Burr C Bazzi S Süle L Otten (1998) ArticleTitleCrown gall of grape: biology of Agrobacterium vitis and the development of disease control strategies Plant Dis 82 1288–1297

    Google Scholar 

  • MD Chilton MH Drummond DJ Merlo D Sciaky AL Montoya MP Gordon EW Nester (1977) ArticleTitleStable incorporation of plasmid DNA into higher plant cells: the moleculer basis of crown gall tumorigenesis Cell 11 263–271 Occurrence Handle890735 Occurrence Handle1:CAS:528:DyaE2sXkvVWgtbw%3D Occurrence Handle10.1016/0092-8674(77)90043-5

    Article  PubMed  CAS  Google Scholar 

  • M Goto Y Takikawa (1984a) ArticleTitleMethods for identification of plant pathogenic bacteria (3) Shokobutsu Boeki (Plant Protection) 38 432–437

    Google Scholar 

  • M Goto Y Takikawa (1984b) ArticleTitleMethods for identification of plant pathogenic bacteria (4) Shokobutsu Boeki (Plant Protection) 38 479–484

    Google Scholar 

  • TC Herlache EW Triplett (2002) ArticleTitleExpression of a crown gall biological control phenotype in an avirulent strain of Agrobacterium vitis by addition of the trifolitoxin production and resistance genes BMC Biotechnol 2 IssueID1 2 Occurrence Handle11882255 Occurrence Handle10.1186/1472-6750-2-2

    Article  PubMed  Google Scholar 

  • A Kawaguchi H Sawada K Inoue H Nasu (2005) ArticleTitleMultiplex PCR for the identification of Agrobacterium biovar 3 strains J Gen Plant Pathol 71 54–59 Occurrence Handle1:CAS:528:DC%2BD2MXktVOnu7g%3D

    CAS  Google Scholar 

  • A Kerr K Htay (1974) ArticleTitleBiological control of crown gall through bacteriocin production Physiol Plant Pathol 4 37–44 Occurrence Handle1:CAS:528:DyaE2cXktFSqurk%3D Occurrence Handle10.1016/0048-4059(74)90042-3

    Article  CAS  Google Scholar 

  • K Kersters J De Ley (1984) Genus III. Agrobacterium Conn 1942 NR Kring JG Holt (Eds) Bergey's manual of systematic bacteriology, vol 1 Williams & Wilkins Baltimore 244–254

    Google Scholar 

  • DJ Lane (1991) 16S/23S rRNA sequencing E Stackebrandt M Goodfellow (Eds) Nucleic acid techniques in bacterial systematics Wiley New York 115–175

    Google Scholar 

  • Y Liang Y Di J Zhao D Ma (1990) ArticleTitleA biotype 3 strain of Agrobacterium radiobacter inhibits crown gall formation on grapevine Acta Microbiol Sin 30 165–171

    Google Scholar 

  • N Matsuo (1993) Bacteriocin S Wakimoto N Matsuyama Y Takanami K Tsuno (Eds) Laboratory guide for plant pathology and microbiology Soft Science Tokyo 459–466

    Google Scholar 

  • LW Moore G Warren (1979) ArticleTitleAgrobacterium radiobacter strain 84 and biological control of crown gall Annu Rev Phytopathol 17 163–179 Occurrence Handle10.1146/annurev.py.17.090179.001115

    Article  Google Scholar 

  • LW Moore H Bouzar T Burr (2001) Agrobacterium NW Schaad JB Jones W Chun (Eds) Laboratory guide for identification of plant pathogenic bacteria EditionNumber3rd ed. APS Press St. Paul, MN 17–35

    Google Scholar 

  • PB New A Kerr (1972) ArticleTitleBiological control of crown gall: field measurements and glasshouse experiments J Appl Bacteriol 35 279–287

    Google Scholar 

  • K Ophel A Kerr (1990) ArticleTitleAgrobacterium vitis sp. nov. for strains of Agrobacterium biovar 3 from grapevines Int J Syst Bacteriol 40 236–241 Occurrence Handle1:CAS:528:DyaK3cXls1yrurg%3D

    CAS  Google Scholar 

  • MA Roy M Sassar (1983) ArticleTitleA medium selective for Agrobacterium tumefaciens biotype 3 Phytopathology 73 810

    Google Scholar 

  • Sawada H (2005) Rhizobium. In: Plant pathogenic bacteria: a reference guide. CABI Publishing, Wallingford, UK (in press)

  • H Sawada K Tsuchiya (2003) ArticleTitleTaxonomy of the genus Agrobacterium Jpn J Phytopathol 69 349–365 Occurrence Handle1:CAS:528:DC%2BD2cXhsFWntrw%3D

    CAS  Google Scholar 

  • H Sawada H Ieki Y Takikawa (1990) ArticleTitleIdentification of grapevine crown gall bacteria isolated in Japan Ann Phytopathol Soc Jpn 56 199–206

    Google Scholar 

  • H Sawada H Ieki H Oyaizu S Matsumoto (1993) ArticleTitleProposal for rejection of Agrobacterium tumefaciens and for revised descriptions for the genus Agrobacterium and for Agrobacterium radiobacter and Agrobacterium rhizogenes Int J Syst Bacteriol 43 694–702 Occurrence Handle8240952 Occurrence Handle1:STN:280:DyaK2c%2FlvFOntA%3D%3D Occurrence Handle10.1099/00207713-43-4-694

    Article  PubMed  CAS  Google Scholar 

  • Schilperoot RA (1972) Integration of Agrobacterium tumefaciens DNA in the genome of crown gall tumor cells and its expression. In: Maas Geesteranus HP (ed) Proceedings of the 3rd international conference on plant pathogenic bacteria. Centre for Agricultural Publishing and Documentation, Wageningen, The Netherlands, pp 223–238

  • JS Shim SK Farrand A Kerr (1987) ArticleTitleBiological control of crown gall: construction and testing of new biocontrol agents Phytopathology 77 463–466 Occurrence Handle1:CAS:528:DyaL2sXhvFWjs7w%3D

    CAS  Google Scholar 

  • JL Staphorst FGH van Zyl BW Strijdom ZE Groenewold (1985) ArticleTitleAgrocin-producing pathogenic and nonpathogenic biotype-3 strains of Agrobacterium tumefaciens active against biotype-3 pathogens Curr Microbiol 12 45–52 Occurrence Handle10.1007/BF01567753

    Article  Google Scholar 

  • J Webster JA Thomson (1986) ArticleTitleAgrocin-producing Agrobacterium tumefaciens strain active against grapevine isolates Appl Environ Microbiol 52 217–219 Occurrence Handle16347112

    PubMed  Google Scholar 

  • C Xianying X Wangnian (1986) ArticleTitleA strain of Agrobacterium radiobacter inhibits growth and gall formation by biotype III strains of Agrobacterium tumefaciens from grapevine Acta Microbiol Sin 26 193–199

    Google Scholar 

  • JM Young LD Kuykendall E Martínez-Romero A Kerr H Sawada (2001) ArticleTitleA revision of Rhizobium Frank 1889, with an emended description of the genus, and the inclusion of all species of Agrobacterium Conn 1942 and Allorhizobium undicola de Lajudie et al. 1998 as new combinations: Rhizobium radiobacter, R. rhizogenes, R. rubi, R. undicola and R. vitis Int J Syst Evol Microbiol 51 89–103 Occurrence Handle11211278 Occurrence Handle1:STN:280:DC%2BD3MzltlCitA%3D%3D

    PubMed  CAS  Google Scholar 

  • JM Young A Kerr H Sawada (2005) Genus II. Agrobacteirum GM Garrity (Eds) Bergey's manual of systematic bacteriology EditionNumber2nd edn, vol 2. Springer New York 340–345

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Laboratory of Plant Pathology and Entomology, Agricultural Experiment Station, Okayama Prefectural General Agriculture Center, Sanyo-cho, Okayama, 709-0801, Japan

    Akira Kawaguchi, Koji Inoue & Hideo Nasu

Authors
  1. Akira Kawaguchi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Koji Inoue
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Hideo Nasu
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Akira Kawaguchi.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kawaguchi, A., Inoue, K. & Nasu, H. Inhibition of crown gall formation by Agrobacterium radiobacter biovar 3 strains isolated from grapevine. J Gen Plant Pathol 71, 422–430 (2005). https://doi.org/10.1007/s10327-005-0234-z

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10327-005-0234-z

Key words

Search

Navigation