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Acinetobacter sp. strain Ths, a novel psychrotolerant and alkalitolerant bacterium that utilizes hydrocarbon

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Abstract

A novel psychrotolerant, alkalitolerant bacterium, strain Ths, was isolated from a soil sample immersed in hot spring water containing hydrocarbons and grown on a chemically defined medium containing n-tetradecane as the sole carbon source. The isolate grew at 0°C but not at temperatures higher than 45°C; its optimum growth temperature was 27°C. It grew in the pH range of 7–9. The strain utilized C13–C30 n-alkane and fluorene at pH 9 and 4°C. To our knowledge, this is the first report on the bacterium that utilizes a wide range of hydrocarbons at a high pH and a low temperature. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain Ths is closely related to genomic species 6 ATCC 17979 (99.1% similarity), genomic species BJ13/TU14 ATCC 17905 (97.8% similarity), genomic species 9 ATCC 9957 (97.6% similarity) belonging to the genus Acinetobacter and to Acinetobacter calcoaceticus JCM 6842T (97.5% similarity). DNA–DNA hybridization revealed that the isolate has 62, 25, 18 and 19% relatedness, respectively, to genomic species 6 ATCC 17979, genomic species BJ13/TU14 ATCC 17905, genomic species 9 ATCC 9957 and A. calcoaceticus, respectively.

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Acknowledgments

The authors thank Dr. A. Nemec for providing A. parvus NIPH 384T and A. ursingii NIPH 137T.

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Authors and Affiliations

  1. Research Institute of Genome-Based Biofactory, National Institute of Advanced Industrial Science and Technology (AIST), Tsukisamu-Higashi, Toyohira-ku, Sapporo, 062-8517, Japan

    Keiko Yamahira, Kikue Hirota, Kenji Nakajima, Naoki Morita & Isao Yumoto

  2. ROM Co., Ltd., Chuo-ku, Sapporo, 062-0804, Japan

    Keiko Yamahira

  3. Laboratory of Electron Microscopy, Graduate School of Dentistry, Hokkaido University, Kita-ku, Sapporo, 060-8586, Japan

    Yoshinobu Nodasaka

  4. Graduate School of Agriculture, Hokkaido University, Kita-ku, Sapporo, 060-8589, Japan

    Isao Yumoto

Authors
  1. Keiko Yamahira
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  2. Kikue Hirota
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  3. Kenji Nakajima
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  4. Naoki Morita
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  5. Yoshinobu Nodasaka
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  6. Isao Yumoto
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Corresponding author

Correspondence to Isao Yumoto.

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Communicated by L. Huang.

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Yamahira, K., Hirota, K., Nakajima, K. et al. Acinetobacter sp. strain Ths, a novel psychrotolerant and alkalitolerant bacterium that utilizes hydrocarbon. Extremophiles 12, 729–734 (2008). https://doi.org/10.1007/s00792-008-0180-8

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  • DOI: https://doi.org/10.1007/s00792-008-0180-8

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