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Acinetobacter tibetensis sp. nov., Isolated from a Soil Under a Greenhouse in Tibet

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Abstract

A Gram-stain-negative, light yellow, aerobic, non-motile, short rod-shaped bacterium named strain Y-23T with iprodione-degrading capability was isolated from a soil under a greenhouse in Tibet, PR China. Strain Y-23T grew at 4–37 ℃ and pH 5.0–9.0 (optimum, 25 ℃ and pH 7.0) with 0–3% (w/v) NaCl (optimum, 0%). Phylogenetic analysis based on 16S rRNA gene and chromosome genome indicated that strain Y-23T formed a stable evolutionary branch with Acinetobacter tandoii DSM 14970T. The 16S rRNA gene similarity, digital DNA-DNA hybridization and average nucleotide identity values between strain Y-23T and Acinetobacter tandoii DSM 14970T were 98.31%, 43.2% and 91.2%, respectively. The genome size was 3.39 Mbp with a genomic DNA G+C content of 40.59 mol%. The predominant fatty acids were C18:1 ω9c, Summed feature 3 (C16:1 ω7c/C16:1 ω6c), C12:0, C12:0 3-OH and C16:0. The polar lipids were diphosphatidyl glycerol, phosphatidyl glycerol, phosphatidyl ethanolamine, phosphatidyl choline, unidentified phospholipid, four unidentified aminophospholipids and two unidentified lipids. The isoprenoid quinone was Q-8 (19.43%) and Q-9 (80.57%). Based on phenotypic, phylogenetic, and genotypic data, strain Y-23T is considered to represent a novel species of the genus Acinetobacter, for which the name Acinetobacter tibetensis sp. nov. is proposed. The type strain is Y-23T (= CICC 25150T = JCM 35630T).

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Data Availability

The 16S rRNA gene sequence and genome of Acinetobacter tibetensis strain Y-23T have been deposited in GenBank.

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Funding

This research was financially supported by the National Natural Science Foundation of China (No. 32060025), the National Natural Science Foundation of Tibet (No. XZ202101ZR0090G), the Training Program for Excellent Young Innovators of Changsha (No. kq2106049), the Provincial College Students Innovative Entrepreneurial Training Plan Program of Hunan, China (No. S202110537006X), the College students’ Innovative Entreneurial Training Plan Program of Hunan Agricultural University, China (No. S202010537078) and the Double First-Class Construction Project of Hunan Agricultural University (No. SYL201802002).

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Authors and Affiliations

  1. College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, 410128, China

    Hu Pan, Jin Li, Hu-Hu Liu, Xiang-Yang Lu & Yun Tian

  2. Institute of Agricultural Product Quality Standard and Testing Research, Tibet Academy of Agricultural and Animal Husbandry Sciences, Lhasa, 850032, China

    Hu Pan & Yi-Fan Zhang

  3. Agricultural and Livestock Products Engineering Technology Research Center of Tibet Autonomous Region, Lhasa, 850032, China

    Hu Pan & Yi-Fan Zhang

  4. Department of Life Sciences, Changzhi University, Changzhi, 046011, China

    Jin Li

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  1. Hu Pan
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  3. Hu-Hu Liu
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Contributions

YT, YZ and HP conceived the project. HP, XL and HL performed the experiments. YZ and HP analyzed the data, and HP and YT drafted and revised the manuscript. All authors have read and approved the final version of the manuscript.

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Correspondence to Yi-Fan Zhang or Yun Tian.

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Pan, H., Li, J., Liu, HH. et al. Acinetobacter tibetensis sp. nov., Isolated from a Soil Under a Greenhouse in Tibet. Curr Microbiol 80, 51 (2023). https://doi.org/10.1007/s00284-022-03158-z

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