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Hymenobacter terrae sp. nov., a Bacterium Isolated from Soil

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Abstract

A Gram-negative, UV tolerant bacterial strain, DG7AT, was isolated from soil samples collected in Seoul city, South Korea. The cells were grown on R2A agar at 25 °C and were pink to red in color. The DNA G+C content of the novel strain DG7A was 63.5 mol%. Chemotaxonomic data revealed that the strains contain the major fatty acids iso-C15:0, anteiso-C15:0, and summed feature 3 (16:1 ω7c/16:1 ω6c), with phosphatidylethanolamine as the major polar lipid. Phylogenetic analysis of the 16S rRNA gene sequences showed that strain DG7AT formed a distinct phylogenetic line along with Hymenobacter soli PB17T, and they shared approximately 98.35 % 16S rRNA gene sequence similarity. However, these two strains shared only 5.3 % pairwise similarity (reciprocal analysis, 36.3 %) in their genomic DNA. The next highest degree of 16S rRNA gene sequence similarity after H. soli PB17T was found with H. glaciei VUG-A130T (96.78 %), H. antarcticus VUG-A42aaT (96.66 %), and H. saemangeumensis GSR0100T (96.57 %). Based on the phylogenetic analysis and analysis of the physiological and biochemical characteristics, this isolate was considered to represent a novel species, for which we propose the name Hymenobacter terrae sp. nov., with type strain DG7AT (= KCTC 32554T = KEMB 9004-164T = JCM 30007T).

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Acknowledgments

We thank Dr. Aidan C. Parte for checking the etymology of the species name. This work was supported by a special research grant from Seoul Women’s University (2015).

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Authors and Affiliations

  1. Department of Bio & Environmental Technology, College of Natural Science, Seoul Women’s University, Seoul, 139-774, Republic of Korea

    Sathiyaraj Srinivasan, Jae-Jin Lee, Kyoung Ryun Park, Se-Hee Park & Myung Kyum Kim

  2. College of Agricultural and Life Sciences, Kyungpook National University, Daegu, 702-701, Republic of Korea

    Hee-Young Jung

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  1. Sathiyaraj Srinivasan
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  2. Jae-Jin Lee
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Correspondence to Hee-Young Jung or Myung Kyum Kim.

Additional information

The NCBI GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain DG7AT (= KCTC 32554T = KEMB 9004-164T = JCM 30007T) is KF862488.

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Srinivasan, S., Lee, JJ., Park, K.R. et al. Hymenobacter terrae sp. nov., a Bacterium Isolated from Soil. Curr Microbiol 70, 643–650 (2015). https://doi.org/10.1007/s00284-014-0760-z

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