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Acinetobacter halotolerans sp. nov., a novel halotolerant, alkalitolerant, and hydrocarbon degrading bacterium, isolated from soil

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Abstract

A novel aerobic, non-motile, halotolerant, alkalitolerant, hydrocarbon degrading, and rod shaped bacterium, designated strain R160T, was isolated from soil in South Korea. Cells were Gram-staining-negative, catalase-positive, and oxidase-negative. This strain grew up to 7% of NaCl and in the pH range of 6–11 (optimum 7.0–10.0). The isolate degraded 51.7 ± 1.3% of hydrocarbon components (C-18, C-20, and C-22) and 45.8 ± 1.4% oil components (kerosene, diesel, and gasoline). Phylogenetic analysis based on 16 S rRNA gene sequences revealed that strain R160T formed a lineage within the genus Acinetobacter, and was closely related to ‘Acinetobacter oleivorans’ DR1T (97.47%, sequence similarity). Other closely related members have sequence similarity between 97.47 to 96.52%. The predominant respiratory lipoquinones of strain R160T were ubiquinone 9 (Q-9) and ubiquinone 8 (Q-8). The major polar lipids were phosphatidylethanolamine (PE), diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), and phosphatidylcholine (PC). The major cellular fatty acids were 9-octadecenoic acid (C18:1 ω9c), hexadecanoic acid (C16:0), and summed feature (comprising C16:1 ω7c and/or C16:1 ω6c). The DNA G + C content of strain R160T was 44.9 mol%. On the basis of phenotypic, genotypic, chemotaxonomic, and phylogenetic characteristics, strain R160T represents a novel species of the genus Acinetobacter, for which the name Acinetobacter halotolerans sp. nov. is proposed. The type strain is R160T (= KEMB 9005-333T = KACC 18453T = JCM 31009T).

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Acknowledgements

This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (2016R1D1A1A09916982).

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Authors and Affiliations

  1. Department of Life Science, College of Natural Sciences, Kyonggi University, Suwon, Gyeonggi-Do, 16227, South Korea

    Ram Hari Dahal, Dhiraj Kumar Chaudhary & Jaisoo Kim

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  1. Ram Hari Dahal
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  2. Dhiraj Kumar Chaudhary
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  3. Jaisoo Kim
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Correspondence to Jaisoo Kim.

Additional information

Communicated by Erko Stackebrandt.

The GenBank/EMBL/DDBJ accession number for the 16S rRNA, rpoB, and gyrB gene sequences of strain R160T are KT032155, KU958712, and KU958711 respectively.

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Dahal, R.H., Chaudhary, D.K. & Kim, J. Acinetobacter halotolerans sp. nov., a novel halotolerant, alkalitolerant, and hydrocarbon degrading bacterium, isolated from soil. Arch Microbiol 199, 701–710 (2017). https://doi.org/10.1007/s00203-017-1349-2

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