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Molecular characterization of intergenic spacer region of 5S ribosomal RNA genes in subgenus Vigna: extensive hybridization among V. unguiculata subspecies

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Abstract

We report the molecular analysis of the 5S ribosomal RNA intergenic spacer (IGS) region from 57 Vigna species of subgenus Vigna. Sequence analysis revealed that the 5S IGS was highly variable in length (189–237 bp) and sequence (58% polymorphic sites). Most of the Vigna species analysed harboured a single type of 5S rRNA repeat unit, except V. unguiculata and V. reticulata that showed multiple ‘intragenomic’ 5S types. The intragenomic 5S types among the six V. unguiculata subspecies were characterized by PCR-RFLP, genomic RFLP and sequencing. The 5S IGS was phylogenetically informative (comparable to ITS-1 and ITS-2 spacers) in inferring species relationships among the Vigna species analysed. However, due to the presence of multiple intragenomic 5S types and their incomplete homogenization among V. unguiculata subspecies the relationships in section Catiang could not be resolved below species level. The results presented indicate that intraspecies hybridization might have resulted in the ‘horizontal transfer’ of 5S types among the V. unguiculata subspecies, while their maintenance could be due to a slow molecular drive.

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References

  • Allaby RG, Brown TA (2001) Network analysis provides insights into evolution of 5S rDNA arrays in Triticum and Aegilopes. Genetics 157:1331–1341

    PubMed  CAS  Google Scholar 

  • Álvarez I, Wendel JF (2003) Ribosomal ITS sequences and plant phylogenetic inference. Mol Phylogenet Evol 29:417–434

    Article  PubMed  Google Scholar 

  • Appel DJ, Gordon TR (1995) Intraspecific variation within populations of Fusarium oxysporum based on RFLP analysis of the intergenic spacer region of the rDNA. Exp Mycol 19:120–128

    Article  PubMed  CAS  Google Scholar 

  • Appels R, Baum BR, Clarke BC (1992) The 5S DNA units of bread wheat (Triticum aestivum). Pl Syst Evol 183:183–194

    Article  CAS  Google Scholar 

  • Ba FS, Pasquet RS, Gepts P (2004) Genetic diversity in cowpea [Vigna unguiculata (L.) Walp.] as revealed by RAPD markers. Genet Res Crop Evol 51:539–550

    Article  CAS  Google Scholar 

  • Bailey CD, Timothy GC, Stephen AH, Colin EH (2003) Characterization of angiosperm nrDNA polymorphism, paralogy, and pseudogenes. Mol Phylogenet Evol 29:435–455

    Article  PubMed  CAS  Google Scholar 

  • Baker WJ, Hedderson TA, Dransfield J (2000) Molecular phylogenetics of Calamus (Palmae) and related Rattan genera based on 5S nrDNA spacer sequence data. Mol Phylogenet Evol 14:218–231

    Article  PubMed  CAS  Google Scholar 

  • Baum BR, Appels R (1992) Evolutionary change at the 5S DNA loci of the species in the Triticeae. Pl Syst Evol 183:195–208

    Article  CAS  Google Scholar 

  • Baum BR, Bailey GL (2001) The 5S rRNA gene sequence variation in wheats and some polyploidy wheat progenitors (Poaceae: Triticeae). Genet Res Crop Evol 48:35–51

    Article  Google Scholar 

  • Baum BR, Johnson DA (1998) The 5S rRNA gene in sea barley (Hordeum marinum Hudson sensu lato): sequence variation among repeat units and relationship to the X haplome in barley (Hordeum). Genome 41:652–661

    PubMed  CAS  Google Scholar 

  • Becerra JX (2003) Evolution of Mexican Bursera (Burseraceae) inferred from ITS, ETS, and 5S nuclear ribosomal DNA sequences. Mol Phylogenet Evol 26:300–309

    Article  PubMed  CAS  Google Scholar 

  • Birky CW, Skarvil RV (1976) Maintenance of genetic homogeneity in systems with multiple genomes. Genet Res 27:249–256

    Article  PubMed  CAS  Google Scholar 

  • Bjourson AJ, Cooper JE (1992) Band-stab PCR: a simple technique for the purification of individual PCR products. Nucleic Acids Res 20:4675

    Article  PubMed  CAS  Google Scholar 

  • Buckler ES, Ippolito A, Holtsford TP (1997) The evolution of ribosomal DNA: divergent paralogues and phylogenetic implications. Genetics 145:821–832

    PubMed  CAS  Google Scholar 

  • Cloix C, Yukawa Y, Sylvie T, Masahiro S, Tourmente S (2003) In vitro analysis of the sequences required for transcription of the Arabidopsis thaliana 5S rRNA genes. Plant J 35:251–261

    Article  PubMed  CAS  Google Scholar 

  • Copenhaver GP, Pikaard CS (1996) Two-dimensional RFLP analyses reveal megabase-sized clusters of rRNA gene variants in Arabidopsis thaliana suggesting local spreading of variants as the mode for gene homogenization during concerted evolution. Plant J 9:273–282

    Article  PubMed  CAS  Google Scholar 

  • Coulibaly S, Pasquet RS, Papa R, Gepts P (2002) AFLP analysis of the phenetic organization and genetic diversity of Vigna unguiculata L. Walp. reveals extensive gene flow between wild and cultivated types. Theor Appl Genet 104:358–366

    Article  PubMed  CAS  Google Scholar 

  • Cox AV, Bennett MD, Dyer TA (1992) Use of polymerase chain reaction to detect spacer size heterogeneity in plant 5S-rRNA gene clusters and to locate such clusters in wheat (Triticum aestivum L.). Theor Appl Genet 83:684–690

    Article  CAS  Google Scholar 

  • Cronn RV, Zhao X, Paterson AH, Wendel JF (1996) Polymorphism and concerted evolution in a tandemly repeated gene family: 5S ribosomal DNA in diploid and allopolyploid cottons. J Mol Evol 42:685–705

    Article  PubMed  CAS  Google Scholar 

  • Danna KJ, Workman R, Coryell V, Keim P (1996) 5S rRNA genes in tribe Phaseoleae: array size, number, and dynamics. Genome 39:445–455

    Article  PubMed  CAS  Google Scholar 

  • Douet J, Tourmente S (2007) Transcription of the 5S rRNA heterochromatic genes is epigenetically controlled in Arabidopsis thaliana and Xenopus laevis. Heredity 99:5–13

    Article  PubMed  CAS  Google Scholar 

  • Dover GA (1986) Molecular drive in multigene families: how biological novelties arise, spread and are assimilated. Trends Genet 6:159–165

    Article  Google Scholar 

  • Drouin G, Moniz de Sá M (1995) The concerted evolution of 5S ribosomal genes linked to the repeat units of other multigene families. Mol Biol Evol 12:481–493

    PubMed  CAS  Google Scholar 

  • Ehlers JD, Hall AE (1997) Cowpea (Vigna unguiculata L Walp). Field Crops Res 53:187–204

    Article  Google Scholar 

  • Farris JS (1989a) The retention index and homoplasy excess. Syst Zool 38:406–407

    Article  Google Scholar 

  • Farris JS (1989b) The retention index and the rescaled consistency index. Cladistics 5:417–419

    Article  Google Scholar 

  • Felsenstein J (1985) Confidence limits of phylogenies: an approach using bootstrap. Evolution 39:783–791

    Article  Google Scholar 

  • Fitch WM (1971) Towards defining the course of evolution: minimum change for a specified tree topology. Syst Zool 20:406–416

    Article  Google Scholar 

  • Fotso M, Azanza JL, Pasquet R, Raymond J (1994) Molecular heterogeneity of cowpea (Vigna unguiculata Fabaceae) seed storage proteins. Pl Syst Evol 191:39–56

    Article  CAS  Google Scholar 

  • Galasso I, Schmidt T, Oignone D, Heslop-Harrison JS (1995) The molecular genetics of Vigna unguiculata (L.) Walp: the physical organization and characterization of 18S-5.8S-25S rRNA genes, 5S rRNA genes, telomere-like sequences and family of centromeric repetitive DNA sequences. Theor Appl Genet 91:928–935

    Article  CAS  Google Scholar 

  • Ganley A, Kobayashi T (2007) Highly efficient concerted evolution in the ribosomal DNA repeats: total rDNA repeat variation revealed by whole-genome shotgun. Genome Res 17:184–191

    Article  PubMed  CAS  Google Scholar 

  • Gornung E, Colangelo P, Annesi F (2007) 5S ribosomal RNA genes in six species of Mediterranean grey mullets: genomic organization and phylogenetic inference. Genome 50:787–795

    Article  PubMed  CAS  Google Scholar 

  • Gottlob-McHugh SG, Levesque M, MacKenzie K, Oslon M, Yarosh O, Johnson DA (1990) Organisation of the 5S rRNA genes in the soybean Glycine max (L.) Merrill and conservation of the 5S repeat structure in higher plants. Genome 33:486–494

    Article  PubMed  CAS  Google Scholar 

  • Halanych KM (1991) 5S ribosomal RNA sequences inappropriate for phylogenetic reconstruction. Mol Biol Evol 8:249–253

    CAS  Google Scholar 

  • Hall TA (1999) BioEdit: a user-friendly sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98

    CAS  Google Scholar 

  • Han OK, Kaga A, Isemura T, Wang XW, Tomooka N, Vaughan DA (2005) A genetic linkage map for azuki bean [Vigna angularis (Willd.) Ohwi & Ohashi]. Theor Appl Genet 111:1278–1287

    Article  PubMed  CAS  Google Scholar 

  • Hemleben V, Werts D (1988) Sequence organization and putative regulatory elements in the 5S rRNA genes of two higher plants (Vigna radiata and Matthiola incana). Gene 62:165–169

    Article  PubMed  CAS  Google Scholar 

  • Hughes KW, Paterson RH (2001) Apparent recombination or gene conversion in the ribosomal ITS region of flammulina (Fungi, Agaricales) hybrids. Mol Biol Evol 18:94–96

    PubMed  CAS  Google Scholar 

  • Kaga A, Ishii T, Tsukimoto K, Tokoro E, Kamijima O (2000) Comparative molecular mapping in Ceratotropis species using an interspecific cross between azuki bean (Vigna angularis) and rice bean (V. umbellata). Theor Appl Genet 100:207–213

    Article  CAS  Google Scholar 

  • Kellogg EA, Appels R (1995) Intraspecific and interspecific variation in the 5S rRNA genes are decoupled in diploid wheat relatives. Genetics 140:325–343

    PubMed  CAS  Google Scholar 

  • Kimura M (1980) A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120

    Article  PubMed  CAS  Google Scholar 

  • Kluge AG, Farris JS (1969) Quantitative phylogenetics and the evolution of anurans. Syst Zool 18:1–32

    Article  Google Scholar 

  • Korn LJ (1982) Transcription of Xenopus 5S ribosomal RNA genes. Nature 295:101–105

    Article  PubMed  CAS  Google Scholar 

  • Kumar S, Tamura K, Nei M (2004) MEGA3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Briefings in Bioinfo 5:150–163

    Article  CAS  Google Scholar 

  • Liao D (1999) Concerted evolution: molecular mechanism and biological implications. Mol Evol 64:24–30

    CAS  Google Scholar 

  • Long EO, Dawid IB (1980) Repeated genes in eukaryotes. Annu Rev Biochem 49:727–764

    Article  PubMed  CAS  Google Scholar 

  • MacKinder B, Pasquet R, Polhill R, Verdcourt B (2001) Tribe 8. Phaseoleae. In: Pope GV, Polhill RM (eds) Flora Zambesiaca, vol. 3, part 5. Royal Botanic Gardens, Kew. pp 1–261.

    Google Scholar 

  • Martins C, Galetti PM Jr (1997) Narrow chromosome diversity in fishes of the genus Schizodon (Characiformes, Anistomidae). Cytobios 92:139–147

    Google Scholar 

  • Martins C, Galetti PM Jr (1999) Chromosomal localization of 5S rDNA genes in Leporinus fish (Anostomidae, Characiformes). Chromosome Res 7:363–367

    Article  PubMed  CAS  Google Scholar 

  • Matyášek R, Fulneček J, Lim KY, Leitch AR, Kovařík A (2002) Evolution of 5S rDNA unit arrays in the plant genus Nicotiana (Solanaceae). Genome 45:556–562

    Article  Google Scholar 

  • Maxted N, Mabuza-Diamini P, Moss H, Padulosi S, Jarvis A, Guarino L (2004) An ecogeographic study: African Vigna. International Plant Genetic Resources Institute, Rome

    Google Scholar 

  • Moran GF, Smith D, Bell JC, Appels R (1992) The 5S RNA in Pinus radiata and the spacer region as a probe for relationships between pinus species. Pl Syst Evol 183:209–221

    Article  CAS  Google Scholar 

  • Muir G, Flemming CC, Schlotterer C (2001) Three divergent rDNA clusters predate the species divergence in Quercus petraea (Matt.) Liebl. and Quercus robur L. Mol Biol Evol 18:112–119

    PubMed  CAS  Google Scholar 

  • Nalini E, Bhagwat SG, Jawali N (2004) A simple method for isolation of DNA from plants suitable for long-term storage and DNA marker analysis. BARC Newsletter 249:208–214

    CAS  Google Scholar 

  • Ohta T (1984) Some models of gene conversion for treating the evolution of multigene families. Genetics 106:517–528

    PubMed  CAS  Google Scholar 

  • Ohta T, Dover GA (1983) Population genetics of multigene families that are dispersed into two or more chromosomes (gene conversion/molecular drive). Proc Natl Acad Sci USA 80:4079–4083

    Article  PubMed  CAS  Google Scholar 

  • Padulosi S (1993) Genetic diversity taxonomy and ecogeographic survey of the wild relatives of cowpea [Vigna unguiculata (L.) Walpers]. Thèse Université catholique. Louvain la Neuve, Belgium

  • Panella L, Gepts P (1992) Genetic relationships within V. unguiculata (L.) Walp. based on isozyme analysis. Genet Res Crop Evol 39:71–88

    Google Scholar 

  • Parkin EJ, Butlin RK (2004) Within and between individual sequence variation among ITS1 copies in the meadow grasshopper Chorthippus parallelus indicates frequent intrachromosomal gene conversion. Mol Biol Evol 21:1595–1601

    Article  PubMed  CAS  Google Scholar 

  • Pasquet RS (1999) Genetic relationships among subspecies of Vigna unguiculata (L.) Walp based on allozyme variation. Theor Appl Genet 98:1104–1119

    Article  CAS  Google Scholar 

  • Pasquet RS (2000) Allozyme diversity of cultivated cowpea V. unguiculata (L.) Walp. Theor Appl Genet 101:211–219

    Article  CAS  Google Scholar 

  • Pasquet RS, Vanderborght T (2000) Isozyme polymorphism in the Vigna frutescens-V. membranacea complex (Tribe Phaseoleae, Fabaceae). Biochem Syst Ecol 28:29–43

    Article  CAS  Google Scholar 

  • Persson C (2000) Phylogeny of Neotropical Alibertia group (Rubiaceae) with emphasis on the genus Alibertia, inferred from ITS and 5S ribosomal DNA sequences. Am J Bot 87:1018–1028

    Article  PubMed  CAS  Google Scholar 

  • Playford J, Appels R, Baum BR (1992) The 5S units of Acacia species (Mimosaceae). Pl Syst Evol 183:235–247

    Article  CAS  Google Scholar 

  • Polanco C, Gonzalez AI, Fuente A, Dover GA (1998) Multigene family of ribosomal DNA in Drosophila melanogaster reveals contrasting patterns of homogenization for IGS and ITS spacer regions: a possible mechanism to resolve this paradox. Genetics 149:243–256

    PubMed  CAS  Google Scholar 

  • Prasad S, Reddy KS, Jawali N (1999) Abundance of random amplified hybridizing microsatellites in mungbean (Vigna radiata L. Wilczek). Asia Pacific J Mol Biol Biotech 7:173–177

    Google Scholar 

  • Röser M, Winterfeld G, Grebenstein B, Hemleben V (2001) Molecular diversity and physical mapping of 5S rDNA in wild and cultivated Oat grasses (Poaceae: Aveneae). Mol Phylogenet Evol 21:198–217

    Article  PubMed  Google Scholar 

  • Saini A, Jawali N (2009) Molecular evolution of 5S rDNA region in Vigna subgenus Ceratotropis and its phylogenetic implications. Pl Syst Evol 280:187–206

    Article  CAS  Google Scholar 

  • Saini A, Reddy KS, Jawali N (2008) Intra-individual and intra-species heterogeneity in nuclear rDNA ITS region of Vigna species from subgenus Ceratotropis. Genet Res 90:299–316

    Article  CAS  Google Scholar 

  • Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425

    PubMed  CAS  Google Scholar 

  • Sajdak SL, Reed KM, Phillips RB (1998) Intraindividual and interspecies variation in the 5S rDNA of coregonid fish. J Mol Evol 46:680–688

    Article  PubMed  CAS  Google Scholar 

  • Sambrook J, Russell DW (2001) Molecular cloning: a laboratory manual, 3rd edn. Cold Spring Harbor Laboratory Press, New York

    Google Scholar 

  • Sanderson MJ, Doyle JJ (1992) Reconstruction of organismal and gene phylogenies from data on multigene families: concerted evolution, homoplasy, and confidence. Syst Biol 41:4–17

    Google Scholar 

  • Sastri DC, Hilu K, Appels R, Lagudah ES, Playford J, Baum BR (1992) An overview of evolution in plant 5S rDNA. Pl Syst Evol 183:169–181

    Article  CAS  Google Scholar 

  • Schlötterer C, Tautz D (1994) Chromosomal homogeneity of Drosophila ribosomal DNA arrays suggests intrachromosomal exchanges drive concerted evolution. Curr Biol 4:777–783

    Article  PubMed  Google Scholar 

  • Schneeberger RG, Creissen GP, Cullis CA (1989) Chromosomal and molecular analysis of the 5S RNA gene organization in flax, Linum usitatissimum. Gene 83:75–84

    Article  PubMed  CAS  Google Scholar 

  • Scoles GJ, Gill BX, Xin ZY, Clarke BC, McIntyre CL, Chapman C, Appels R (1988) Frequent duplications and deletion events in the 5S RNA genes and the associated spacer regions in the Triticeae. Pl Syst Evol 160:105–122

    Article  CAS  Google Scholar 

  • Shi L, Zhu T, Keim P (1996) Ribosomal RNA genes in soybean and common bean: chromosomal organization, expression and evolution. Theor Appl Genet 93:136–141

    Article  CAS  Google Scholar 

  • Singh BB, MohanRaj DR, Dashiell KE, Jackai L (1997) Advances in cowpea research. IITA-JIRCAS, Ibadan

  • Smith GP (1976) Evolution of repeated DNA sequences by unequal crossover. Science 191:528–535

    Article  PubMed  CAS  Google Scholar 

  • Sonnante G, Spinosa A, Marangi A, Pignone D (1997) Isozyme and RAPD analysis of the genetic diversity within and between Vigna luteola and V. marina. Ann Bot 80:741–746

    Article  CAS  Google Scholar 

  • Southern EM (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98:503–517

    Article  PubMed  CAS  Google Scholar 

  • Steele KP, Holsinger KE, Jansen RK, Taylor DW (1991) Assessing the reliability of 5S rRNA sequence data for phylogenetic analysis in green plants. Mol Biol Evol 8:240–248

    CAS  Google Scholar 

  • Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The ClustalX Windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 24:4876–4882

    Article  Google Scholar 

  • Thulin M, Lavin M, Pasquet R, Delgado-Salinas A (2004) Phylogeny and biography of Wajira (Leguminosae): a monophyletic segregate of Vigna centered in the Horn of Africa region. Syst Bot 29:903–920

    Article  Google Scholar 

  • Tyler BM (1986) Transcription of Neurospora crassa 5S rRNA genes require a TATA box and three internal elements. J Mol Evol 196:801–811

    Google Scholar 

  • Udovicic F, Mcfadden GI, Ladiges PY (1995) Phylogeny of Eucalyptus and Angophora based on the 5S rDNA spacer sequence data. Mol Phylogenet Evol 4:247–256

    Article  PubMed  CAS  Google Scholar 

  • Vaillancourt RE, Weeden NF (1992) Chloroplast DNA polymorphism suggests a Nigerian center of domestication for the cowpea Vigna unguiculata (Leguminosae). Am J Bot 79:1194–1199

    Article  CAS  Google Scholar 

  • Vaillancourt RE, Weeden NF, Barnard J (1993) Isozyme diversity in the cowpea species complex. Crop Sci 33:606–613

    Article  CAS  Google Scholar 

  • Van der Maesen LJG, Pasquet R (2006) Vigna Savi. In: Akoegninou A, Van der Burg WJ, Van der Maesen LJG (eds) Flore Analytique du Benin. Backhuis, Wageningen, pp 734–747

    Google Scholar 

  • Vaughan DA, Tomooka N, Kaga A, Doi K, Yoon MS (2000) Research on Vigna subgenus Ceratotropis genetic resources that provide insights into in situ conservation strategies. Proceedings of the 7th MAFF International Workshop on Genetic Resources. Wild Legumes. MAFF, NIAR, Tsukuba, Japan, pp 129–150

  • Venkateswarlu K, Lee SW, Nazar RN (1991) Conserved upstream sequence elements in plant 5S ribosomal RNA-encoding genes. Gene 105:249–253

    Article  PubMed  CAS  Google Scholar 

  • Vijaykumar A, Saini A, Jawali N (2010) Phylogenetic analysis of subgenus Vigna species using ribosomal RNA ITS: evidence of hybridization among V. unguiculata subspecies. J Hered 101:177–188

    Article  PubMed  CAS  Google Scholar 

  • Volkov RA, Borisjuk NV, Panchuk II, Schweizer D, Hemleben V (1999) Elimination and rearrangement of parental rDNA in the allotetraploid Nicotiana tabacum. Mol Biol Evol 16:311–320

    PubMed  CAS  Google Scholar 

  • Volkov RA, Zanke C, Panchuk II, Hemleben V (2001) Molecular evolution of 5S rDNA of Solanum species (sect. Petota): application for molecular phylogeny and breeding. Theor Appl Genet 103:1273–1282

    Article  CAS  Google Scholar 

  • Volkov RA, Komarova NY, Hemleben V (2007) Ribosomal DNA in plant hybrids: inheritance, rearrangement, expression. Syst Biodivers 5:261–276

    Article  Google Scholar 

  • Wasko AP, Martins C, Wright JM, Galetti PM Jr (2001) Molecular organization of 5S rDNA in fishes of the genus Brycon. Genome 44:893–902

    PubMed  CAS  Google Scholar 

  • Yang Y, Tseng P, Tail P, Chang C (1998) Phylogenetic position of Raphanus in relation to Brassica species based on 5S rRNA spacer sequence data. Bot Bull Acad Sin 39:153–160

    CAS  Google Scholar 

  • Zhang D, Sang T (1998) Physical mapping of ribosomal RNA genes in peonies (Paeonia; Paeoniaceae) by fluorescent in situ hybridization: implications for phylogeny and concerted evolution. Am J Bot 86:735–740

    Article  PubMed  CAS  Google Scholar 

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Acknowledgments

We thank Dr. Thierry Vanderborght, Seed Bank Manager, National Botanic Garden, Belgium for providing the plant materials used in this study. We thank Dr. S.K. Apte, Head, Molecular Biology Division, Bhabha Atomic Research Centre, Mumbai, India, for encouragement and support during this study.

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  1. Molecular Biology Division, Bhabha Atomic Research Centre, Trombay, Mumbai, 400 085, India

    Archana Vijaykumar, Ajay Saini & Narendra Jawali

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Vijaykumar, A., Saini, A. & Jawali, N. Molecular characterization of intergenic spacer region of 5S ribosomal RNA genes in subgenus Vigna: extensive hybridization among V. unguiculata subspecies. Plant Syst Evol 294, 39–55 (2011). https://doi.org/10.1007/s00606-011-0446-y

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