Skip to main content
SpringerLink
Log in

Molecular evolution of 5S rDNA region in Vigna subgenus Ceratotropis and its phylogenetic implications

  • Original Article
  • Published:
Plant Systematics and Evolution Aims and scope Submit manuscript

Abstract

The evolution of 5S rRNA gene unit (5S gene unit) was studied among the ten species belonging to Vigna subgenus Ceratotropis by sequencing and analyzing the intra- and inter-specific sequence heterogeneity. The 5S unit from these species ranged from 214 to 342 bp in length as a result of several indels in the intergenic spacer (IGS) region. A large deletion (>100 bp) was found specifically in the IGS of V. radiata accessions. IGS showed high sequence variation with more than 50% polymorphic and 35.4% parsimony informative sites. However, the coding region (5S gene) was highly conserved, both in length and in sequence. Intra-genomic and intra-specific divergence was observed among some species, which indicated that the 5S unit is evolving at different rates among the Vigna species. Most Vigna species harbored one type of 5S unit indicating complete homogenization among them. Vigna glabrescens, a tetraploid species, also showed single type of 5S rDNA from only one of the diploid progenitor indicating loss or homogenization of the other type. However, V. nakashimae and V. riukiuensis harbored multiple, diverse, ‘intra-genomic 5S types’ indicating that 5S rDNA is not completely homogenized by concerted evolution and is still evolving. In general, the phylogeny based on IGS sequences was in agreement with many of the earlier reports except some surprising observations such as, V. glabrescens clustered with V. mungo in section Ceratotropis and unlike most of the species, wild and cultivated types of V. umbellata were present in different subclusters. Presence of divergent 5S sequences in V. nakashimae and V. riukiuensis caused errors in phylogeny reconstruction at species level and suggested a horizontal ‘gene transfer’ as a result of inter-species hybridization. The comparative analysis showed that 5S IGS sequences have better phylogenetic utility than chloroplast DNA sequences, such as atpB-rbcL and is comparable to ITS1 and ITS2 in this respect.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7

Similar content being viewed by others

References

  • Allaby RG, Brown TA (2001) Network analysis provides insights into evolution of 5S rDNA arrays in Triticum and Aegilops. Genetics 157:1331–1341

    PubMed  CAS  Google Scholar 

  • Appels R, Clarke BC (1992) The 5S DNA unit of bread wheat (Triticum aestivum). Pl Syst Evol 183:195–208

    Article  Google Scholar 

  • Appels R, Reddy P, McIntyre CL, Moran LB, Frankel OH, Clarke BC (1989) The molecular-cytogenetic analysis of grasses and its applications to studying relationships among species of the Triticeae. Genome 31:122–133

    PubMed  CAS  Google Scholar 

  • Appels R, Baum BR, Clarke BC (1992) The 5S DNA units of bread wheat (Triticum aestivum). Pl Syst Evol 183:183–194

    Article  CAS  Google Scholar 

  • Arora RK (1985) Diversity and collection of wild Vigna species in India. FAO/IBPGR Plant Genet Resour Newslett 63:26–33

    Google Scholar 

  • Baker WJ, Hedderson TA, Dransfield J (2000) Molecular phylogenetics of Calamus (Palmae) and related Rattan genera based on 5S nrDNA spacer sequence data. Molec Phylogenet Evol 14(2):218–231

    Article  PubMed  CAS  Google Scholar 

  • Barciszewska MZ, Erdmann VA, Barciszewski J (1994a) A new type of RNA editing. 5S ribosomal DNA transcripts are edited to mature 5S rRNA. Biochem Molec Biol Int 34:437–448

    PubMed  CAS  Google Scholar 

  • Barciszewska MZ, Szymanski M, Specht T, Erdmann VA, Barciszewski J (1994b) Compilation of plant 5S ribosomal RNA sequences on RNA and DNA levels. Pl Sci 100:117–128

    Article  CAS  Google Scholar 

  • Barciszewska MZ, Szymanski M, Erdmann VA, Barciszewski J (2001) Structure and functions of 5S rRNA. Acta Biochim Pol 48:191–198

    PubMed  CAS  Google Scholar 

  • Baudoin JP, Maréchal R (1988) Taxonomy and Evolution of the genus Vigna. In Proceeding of the Second International Mungbean Symposium. Asian Vegetable Res. Dev. Center, Shanhua, Taiwan. pp 2–12

  • Baum BR, Appels R (1992) Evolutionary change at the 5S DNA loci of the species in the Triticeae. Pl Syst Evol 183:195–208

    Article  CAS  Google Scholar 

  • Baum BR, Johnson DA (1998) The 5S rRNA gene in sea barley (Hordeum marinum Hudson sensu lato): Sequence variation among repeat units and relationship to the X haplome in barley (Hordeum). Genome 41:652–661

    Article  PubMed  CAS  Google Scholar 

  • Becerra JX (2003) Evolution of Mexican Bursera (Burseraceae) inferred from ITS, ETS, and 5S nuclear ribosomal DNA sequences. Molec Phylogenet Evol 26:300–309

    Article  PubMed  CAS  Google Scholar 

  • Birky CW, Skarvil RV (1976) Maintenance of genetic homogeneity in systems with multiple genomes. Genet Res 27:249–256

    Article  PubMed  CAS  Google Scholar 

  • Bjourson AJ, Cooper JE (1992) Band-stab PCR: a simple technique for the purification of individual PCR products. Nucleic Acids Res 20:4675

    Article  PubMed  CAS  Google Scholar 

  • Brockmann C, Nilsson T, Gabrielsen TM (1996) A classic example of postglacial allopolyploid speciation re-examined using RAPD markers and nucleotide sequences: Saxifraga osloensis (Saxifragaceae). Symb Bot Upsala 31:74–89

    Google Scholar 

  • Buckler ESIV, Ippolito A, Holtsford TP (1997) The evolution of ribosomal DNA: divergent paralogues and phylogentic implications. Genetics 145:821–832

    PubMed  CAS  Google Scholar 

  • Capesius I (1993) Sequence of the 5S rRNA gene from Brassica nigra and its relation to other 5S rRNA genes from Brassicacea. Pl Physiol 142:112–114

    CAS  Google Scholar 

  • Chen HK, Mok MC, Shanmugsundaram S, Mok DWS (1983) Interspecific hybridization between Vigna radiata (L.) Wilczek and V. glabrescens. Theor Appl Genet 78:641–647

    Google Scholar 

  • Cox AV, Bennett MD, Dyer TA (1992) Use of polymerase chain reaction to detect spacer size heterogeneity in plant 5S-rRNA gene clusters and to locate such clusters in wheat (Triticum aestivum L.). Theor Appl Genet 83:684–690

    Article  CAS  Google Scholar 

  • Cronn RV, Zhao X, Paterson AH, Wendel JF (1996) Polymorphism and concerted evolution in a tandemly repeated gene family: 5S ribosomal DNA in diploid and allopoloyploid cottons. J Molec Evol 42:685–705

    Article  PubMed  CAS  Google Scholar 

  • Dana S (1980) Genomic relationship in genus Vigna and its implications in breeding programme. In: Gill KS (ed) Breeding methods for the improvement of pulse crops. Punjab Agr Univ, Ludhiana, pp 357–367

    Google Scholar 

  • Dana S, Karmakar PG (1990) Species relation in Vigna subgenus Ceratotropis and its implications in breeding. In: Janick J (ed) Plant breeding review. Timber Press, Port land, pp 19–42

    Google Scholar 

  • Danna KJ, Workman R, Coryell V, Keim P (1996) 5S rRNA genes in tribe Phaseoleae: array size, number, and dynamics. Genome 39:445–455

    Article  PubMed  CAS  Google Scholar 

  • Doi K, Kaga A, Tomooka N, Vaughan DA (2002) Molecular phylogeny of genus Vigna subgenus Ceratotropis based on rDNA ITS and atpB-rbcL intergenic spacer of cpDNA sequences. Genetica 114:129–145

    Article  PubMed  CAS  Google Scholar 

  • Dover GA (1986) Molecular drive in multigene families: how biological novelties arise, spread and are assimilated. Trends Genet 6:159–165

    Article  Google Scholar 

  • Dubcovsky J, Dvorak J (1995) Ribosomal RNA multigene loci: nomads of the Triticeae genomes. Genetics 140:1367–1377

    PubMed  CAS  Google Scholar 

  • Egawa Y, Nakagahra M, Fernandez GCJ (1988) Cytogenetical analysis of tetraploid Vigna glabrescens by interspecific hybridization involving diploid Asian Vigna species. In: Proceedings of the second international mungbean symposium. Asian vegetable Research and Devevolpment Center, Shanhua, Taiwan, pp 200–204

  • Egawa Y, Chotechuen S, Tomooka N, Lairungreang C, Nakeeraks P, Thavarasook C, Kitbamroong C (1996) Collaborative research program on mungbean germplasm (subgenus Ceratotropis of the genus Vigna) between DOA, Thailand and JIRCAS, Japan. In: Srinivas P, Kitbamroong C, Miyazaki S (eds) Mungbean germplasm: collection, evaluation and utilization for breeding program. JIRCAS, Tsukuba, pp 1–8

    Google Scholar 

  • Farris JS (1989a) The retention index and homoplasy excess. Syst Zool 38:406–407

    Article  Google Scholar 

  • Farris JS (1989b) The retention index and the rescaled consistency index. Cladistics 5:417–419

    Article  Google Scholar 

  • Fatokun CA, Danesh D, Young ND, Stewert EL (1993) Molecular taxonomic relationship in the genus Vigna based on the RFLP analysis. Theor Appl Genet 86:97–104

    Article  CAS  Google Scholar 

  • Felsenstein J (1985) Confidence limits on phylogenies: an approach using bootstrap. Evolution 39:783–791

    Article  Google Scholar 

  • Fitch WM (1971) Towards defining the course of evolution: minimum change for a specified tree topology. Syst Zool 20:406–416

    Article  Google Scholar 

  • Galasso I, Schmidt T, Oignone D, Heslop-Harrison JS (1995) The molecular genetics of Vigna unguiculata (L.) Walp: the physical organization and characterization of 18S–5.8S–25S rRNA genes, 5S rRNA genes, telomere-like sequences and family of centromeric repetitive DNA sequences. Theor Appl Genet 91:928–935

    Article  CAS  Google Scholar 

  • Goel S, Raina SN, Ogihara Y (2002) Molecular evolution and phylogenetic implications of internal transcribed spacer sequences of nuclear ribosomal DNA in the Phaseolus–Vigna Complex. Molec Phylogenet Evol 22:1–19

    Article  PubMed  CAS  Google Scholar 

  • Gopinathan MC, Babu CR (1986) A unique growth pattern associated with cleistogamy in a tropical legume, Vigna minima (Roxb.) Ohwi & Ohashi (Leguminosae). Bot J Linn Soc 92:263–268

    Article  Google Scholar 

  • Gottlob-McHugh SG, Levesque M, MacKenzie K, Oslon M, Yarosh O, Johnson DA (1990) Organisation of the 5S rRNA genes in the soybean Glycine max (L.) Merrill and conservation of the 5S repeat structure in higher plants. Genome 33:486–494

    PubMed  CAS  Google Scholar 

  • Halanych KM (1991) 5S ribosomal RNA sequences in appropriate for phylogenetic reconstruction. Molec Biol Evol 8:249–253

    CAS  Google Scholar 

  • Hanson RE, Islam-Faridi MN, Percival EA, Crane CF, Ji Y, McKnight TD, Stelly DM, Price HJ (1996) Distribution of 5S and 18S–2S rDNA loci in a tetraploid cotton (Gossypium hirsutum L.) and its putative diploid ancestors. Chromosoma 105:55–61

    Article  PubMed  CAS  Google Scholar 

  • Hara H (1955) Critical notes on some type specimen of East-Asiatic plants in foreign herbaria. J Jpn Bot 30:138–141

    Google Scholar 

  • Hemleben V, Werts D (1988) Sequence organization and putative regulatory elements in the 5S rRNA genes of two higher plants (Vigna radiata and Matthiola incana). Gene 62:165–169

    Article  PubMed  CAS  Google Scholar 

  • Hori J, Osawa H (1988) Origin and evolution of organisms as deduced from 5S ribosomal RNA sequences. Molec Biol Evol 4:445–472

    Google Scholar 

  • Hughes KW, Paterson RH (2001) Apparent recombination or gene conversion in the ribosomal ITS region of flammulina (Fungi, Agaricales) hybrids. Molec Biol Evol 18:94–96

    PubMed  CAS  Google Scholar 

  • Jaaska V, Jaaska V (1990) Isozyme variation in Asian beans. Bot Acta 103:281–290

    CAS  Google Scholar 

  • Jobst J, King K, Hemleben V (1998) Molecular evolution of the internal transcribed spacers (ITS and ITS2) and phylogenetic relationships among the species of the family Cucurbitaceae. Molec Phylogenet Evol 9:204–219

    Article  PubMed  CAS  Google Scholar 

  • Kaga A, Tomooka N, Egawa Y, Hosaka K, Kamijima O (1996) Species relation ship in the subgenus Ceratotropis (genus Vigna) as revealed by RAPD analysis. Euphytica 88:17–24

    Article  CAS  Google Scholar 

  • Kellogg EA, Appels R (1995) Intraspecific and interspecific variation in the 5S rRNA genes are decoupled in diploid wheat relatives. Genetics 140:325–343

    PubMed  CAS  Google Scholar 

  • Kimura M (1980) A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Molec Evol 16:111–120

    Article  PubMed  CAS  Google Scholar 

  • Kluge AG, Farris JS (1969) Quantitative phylogenetics and the evolution of anurans. Syst Zool 18:1–32

    Article  Google Scholar 

  • Konarev AV, Tomooka N, Vaughan DA (2002) Proteinase inhibitor polymorphism in the genus Vigna subgenus Ceratotropis and its biosystematic implications. Euphytica 123:165–177

    Article  CAS  Google Scholar 

  • Korn LJ (1982) Transcription of Xenopus 5S ribosomal RNA genes. Nature 295:101–105

    Article  PubMed  CAS  Google Scholar 

  • Krishna TG, Jawali N (1997) DNA isolation from single or half seeds suitable for random amplified polymorphic DNA analysis. Anal Biochem 250:125–127

    Article  PubMed  CAS  Google Scholar 

  • Kumar S, Tamura K, Jakobsen IB, Nei M (2001) MEGA2: molecular evolutionary genetics analysis software. Bioinformatics 17(12):1244–1245

    Article  PubMed  CAS  Google Scholar 

  • Long EO, David IB (1980) Repeated genes in eukaryotes. Annual Rev Biochem 49:727–764

    Article  CAS  Google Scholar 

  • Maekawa F (1955) Topo-morphological and taxonomical studies in Phaseoleae, Leguminosae. Jpn J Bot 15:103–116

    Google Scholar 

  • Maréchal R, Mascherpa JM, Stainier F (1978) Etude taxonomique d’un groupe complexe d’espéces des genres Phaseolus et Vigna (Papilionaceae) sur labase de donnes morphologiques et polliniques, traitees par l’analyse informatique. Biossiera Geneve 28:1–273

    Google Scholar 

  • Maréchal R, Mascherpa JM, Stainier F (1981) Taxonometric study of the Phaseolus–Vigna complex and related genera. In: Polhill RM, Raven PH (eds) Advances in legume systematics. Royal Botanic Gardens, Kew, pp 301–327

    Google Scholar 

  • McIntyre CL, Winberg B, Houchins K, Appels R, Baum BR (1992) Relationships between Oryza (Poaceae) species based on 5S DNA sequences. Pl Syst Evol 183:249–264

    Article  CAS  Google Scholar 

  • Moran GF, Smith D, Bell JC, Appels R (1992) The 5S RNA in Pinus radiata and the spacer region as a probe for relationships between pinus species. Pl Syst Evol 183:209–221

    Article  CAS  Google Scholar 

  • Muir G, Flemming CC, Schlotterer C (2001) Three divergent rDNA clusters predate the species divergence in Quercus petraea (Matt.) Liebl. and Quercus robur L. Molec Biol Evol 18:112–119

    PubMed  CAS  Google Scholar 

  • Nei M, Kumar S (2000) Molecular evolution and phylogenetics. Oxford University Press, New York

    Google Scholar 

  • Nicholas KB, Nicholas HB Jr, Deerfield DWII (1997) GeneDoc: analysis and visualization of genetic variation. EMBNEW NEWS 4:14

    Google Scholar 

  • Nolte RT, Conkin RM, Harrison SC, Brown RS (1998) Differing roles for zinc fingers in DNA recognition: structure of a six-finger transcription factor IIIA complex. Proc Natl Acad Sci USA 95:2938–2943

    Article  PubMed  CAS  Google Scholar 

  • Ohta T (1984) Some models of gene conversion for treating the evolution of multigene families. Genetics 106:517–528

    PubMed  CAS  Google Scholar 

  • Ohwi J, Ohashi J (1969) Adzuki beans of Asia. Jpn J Bot 44:29–31

    Google Scholar 

  • Persson C (2000) Phylogeny of Neotropical Alibertia group (Rubiaceae), with emphasis on the genus Alibertia, inferred from ITS and 5S ribosomal DNA sequences. Amer J Bot 87:1018–1028

    Article  CAS  Google Scholar 

  • Pieler T, Hamm J, Roeder RG (1987) The 5S gene internal control region is composed of three distinct sequence elements organized as two functional domains with variable spacing. Cell 16:91–100

    Article  Google Scholar 

  • Playford J, Appels R, Baum BR (1992) The 5S units of Acacia species (Mimosaceae). Pl Syst Evol 183:235–247

    Article  CAS  Google Scholar 

  • Potokina E, Vaughan DA, Eggi EE, Tomooka N (2000) Population diversity of Vicia sativa agg. (Fabaceae) in the flora of the former USSR deduced from RAPD and seed protein analyses. Genet Resources Crop Evol 47:171–183

    Article  Google Scholar 

  • Prasad S, Reddy KS, Jawali N (1999) Abundance of random amplified hybridizing microsatellites in mungbean (Vigna radiata L. Wilzeck). Asia Pacific J Molec Biol and Biotechnology 7:173–177

    Google Scholar 

  • Reddy P, Appels R (1989) A second locus for the 5S multigene family in Secale L: sequence divergence in two lineages of the family. Genome 32:456–467

    CAS  Google Scholar 

  • Roelofs D, van Velzen J, Kupreus P, Bachmann K (1997) Molecular evidence for an extinct parent of the tetraploid species Microseris acuminata and M. campestris (Asteraceae, Lactuceae). Molec Ecol 6:641–649

    Article  CAS  Google Scholar 

  • Röser M, Winterfeld G, Grebenstein B, Hemleben V (2001) Molecular diversity and physical mapping of 5S rDNA in wild and cultivated Oat grasses (Poaceae: Aveneae). Molec Phylogenet Evol 21(2):198–217

    Article  PubMed  CAS  Google Scholar 

  • Saini A, Reddy KS, Jawali N (2008) Intra-individual and intra-species heterogeneity in nuclear rDNA ITS region of Vigna species from subgenus Ceratotropis. Genet Res 90:299–316

    Article  PubMed  CAS  Google Scholar 

  • Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Molec Biol Evol 4:406–425

    PubMed  CAS  Google Scholar 

  • Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbour Laboratory Press, Cold Spring Harbour

    Google Scholar 

  • Sanderson MJ, Doyle JJ (1992) Reconstruction of organismal and gene phylogenies from data on multigene families: Concerted evolution, homoplasy and confidence. Syst Biol 41:4–17

    Google Scholar 

  • Sang T, Crawford DJ, Stuessy TF (1995) Documentation of reticulate evolution in peonies (Paeonia) using internal transcribed spacer sequences of nuclear ribosomal DNA: implications for biogeography and concerted evolution. Proc Natl Acad Sci USA 92:6813–6817

    Article  PubMed  CAS  Google Scholar 

  • Sastri DC, Hilu K, Appels R, Lagudah ES, Playford J, Baum BR (1992) An overview of evolution in plant 5S rDNA. Pl Syst Evol 183:169–181

    Article  CAS  Google Scholar 

  • Sawa M (1983) Study on the breeding of azuki bean (V. angularis) by interspecific crossing. Michurin Biol Res 18:3–26

    Google Scholar 

  • Schneeberger RG, Creissen GP, Cullis CA (1989) Chromosomal and molecular analysis of the 5S RNA gene organization in flax, Linum usitatissimum. Gene 83:75–84

    Article  PubMed  CAS  Google Scholar 

  • Scoles GJ, Gill BX, Xin ZY, Clarke BC, McIntyre CL, Chapman C, Appels R (1988) Frequent duplications and deletion events in the 5S RNA genes and the associated spacer regions in the Triticeae. Pl Syst Evol 160:105–122

    Article  CAS  Google Scholar 

  • Shi L, Zhu T, Keim P (1996) Ribosomal RNA genes in soybean and common bean: chromosomal organization, expression and evolution. Theor Appl Genet 93:136–141

    Article  CAS  Google Scholar 

  • Siriwardhane D, Egawa Y, Tomooka N (1991) Cross compatibility of cultivated adzuki bean (Vigna angularis) and rice bean (V. umbellata) with their wild relatives. Pl Breed 107:320–325

    Article  Google Scholar 

  • Smith GP (1976) Evolution of repeated DNA sequences by unequal crossover. Science 191:528–535

    Article  PubMed  CAS  Google Scholar 

  • Snowdown RJ, Köhler W, Köhler A (1997) Chromosomal localization and characterization of rDNA loci in the Brassica A and C genomes. Genome 40:582–587

    Article  Google Scholar 

  • Steele KP, Holsinger KE, Jansen RK, Taylor DW (1991) Assessing the reliability of 5S rRNA sequence data for phylogenetic analysis in green plants. Molec Biol Evol 8:240–248

    CAS  Google Scholar 

  • Tateishi Y (1985) A revision of the Azuki bean group, the subgenus Ceratotropis of the genus Vigna (leguminosae). PhD Dissertion, Tohoku University, Japan

  • Thomas HM, Harper JA, Meredith MR, Morgan WG, King IP (1997) Physical mapping of ribosomal DNA sites in Festuca arundinacea and related species by in situ hybridization. Genome 40:406–410

    Article  PubMed  CAS  Google Scholar 

  • Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The ClustalX windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 24:4876–4882

    Article  Google Scholar 

  • Tomooka N, Egawa Y, Kaga A (2000) Biosysytematics and genetic resources of the genus Vigna subgenus Ceratotropis. In: 17th MAFF international workshop on genetic resources. Wild legumes. National Institute of Agrobilogical Resources, Tsukuba, Japan, pp 37–62

  • Tomooka N, Maxted C, Thavarasook C, Jayasurya AHM (2002) AFLP analysis of diploid Vigna subgenus Ceratotropis species. Genet Res Crop Evol 49(5):521–530

    Article  Google Scholar 

  • Tomooka N, Vaughan DA, Moss H, Maxted N (2003) The Asian Vigna: Genus Vigna subgenus Ceratotropis genetic resources. Kluwer, Dordrecht

  • Tyler BM (1986) Transcription of Neurospora crassa 5S rRNA genes require a TATA box and three internal elements. J Mol Evol 196:801–811

    Google Scholar 

  • Udovicic F, Mcfadden GI, Ladiges PY (1995) Phylogeny of Eucalyptus and Angophora based on the 5S rDNA spacer sequence data. Molec Phylogenet Evol 4:247–256

    Article  PubMed  CAS  Google Scholar 

  • Van Houten WHJ, Scarlett N, Bachmann K (1993) Nuclear DNA markers of the Australian tetraploid Microseris scapigera and its North American diploid relatives. Theor Appl Genet 78:498–505

    Google Scholar 

  • Vaughan HE, Jamilena M, Ruiz Rejon C, Parker JS, Garrido-Ramos MA (1993) Loss of nucleolus-organizer regions during polyploid evolution in Scilla autumnalis. Heredity 71:574–580

    Article  Google Scholar 

  • Vaughan DA, Tomooka N, Kaga A, Doi K, Yoon MS (2000) Research on Vigna subgenus Ceratotropis genetic resources that provide insights into in situ conservation strategies. In: Proceeding of the 7th MAFF international workshop on genetic resources. Wild Legumes. MAFF, NIAR, Tsukuba, Japan, pp 129–150

  • Venkateswarlu K, Lee SW, Nazar RN (1991) Conserved upstream sequence elements in plant 5S ribosomal RNA-encoding genes. Gene 105:249–253

    Article  PubMed  CAS  Google Scholar 

  • Verdcourt B (1970) Studies in the Leguminosae–Papilionoideae for the ‘Flora of Tropical East Africa’ IV. Kew Bull 24:507–569

    Article  Google Scholar 

  • Volkov RA, Borisjusk NV, Panchuk II, Schweizer D, Hemleben V (1999) Elimination and rearrangement of parental rDNA in the allotetraploid Nicotiana tabacum. Molec Bio Evol 16:311–320

    CAS  Google Scholar 

  • Volkov RA, Zanke C, Panchuk II, Hemleben V (2001) Molecular evolution of 5S rDNA of Solanum species (sect. Petota): application for molecular phylogeny and breeding. Theor Appl Genet 103:1273–1282

    Article  CAS  Google Scholar 

  • Wendel JF (2000) Genome evolution in polyploids. Pl Molec Biol 42:225–249

    Article  CAS  Google Scholar 

  • Wendel JF, Schnabel A, Seelanan T (1995) Bidirectional interlocus concerted evolutionfollowing allopolyploid speciation in cotton (Gossypium). Proc Natl Acad Sci USA 92:280–284

    Article  PubMed  CAS  Google Scholar 

  • Wolffe AP (1994) The role of transcription factors, chromatin structure and DNA replication in 5S RNA gene regulation. J Cell Sci 107:2055–2063

    PubMed  CAS  Google Scholar 

  • Xia X, Xie Z (2001) DAMBE: data analysis in molecular biology and evolution. J Heredity 92:371–373

    Article  CAS  Google Scholar 

  • Yamaguchi H (1992) Wild and weed azuki beans in Japan. Econ Bot 46:384–394

    Google Scholar 

  • Yang YW, Tseng PF, Tai PY, Chang CJ (1998) Phylogenetic position of Raphanus in relation to Brassica species. Bot Bull Acad Sin 38:153–160

    Google Scholar 

  • Yoon MS, Doi K, Kaga A, Tomooka N, Vaughan DA (2000) Analysis of genetic diversity in the Vigna minima complex and related species in East Asia. J Pl Res 113:375–386

    Article  Google Scholar 

  • Zanke C, Borisjuk N, Ruoss B, Schilde-Rentschler L, Ninnemann H, Hemleben V (1995) A specific oligonucleotide of the 5S rDNA spacer and species specific elements identify symmetric somatic hybrids between Solanum tuberosum and S. pinnatisectum. Theor Appl Genet 90:720–726

    Article  Google Scholar 

  • Zhang D, Sang T (1998) Physical mapping of ribosomal RNA genes in peonies (Paeonia; Paeoniaceae) by fluorescent in situ hybridization: implications for phylogeny and concerted evolution. Amer J Bot 735–740

Download references

Acknowledgments

Grateful thanks are due to Dr. T. Vanderborght, National Botanic Garden of Belgium from whom we obtained the seed material used in this study.

Author information

Authors and Affiliations

  1. Molecular Biology Division, Bhabha Atomic Research Centre, Trombay, Mumbai, 400 085, India

    Ajay Saini & Narendra Jawali

Authors
  1. Ajay Saini
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Narendra Jawali
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Narendra Jawali.

Electronic supplementary material

Rights and permissions

Reprints and permissions

About this article

Cite this article

Saini, A., Jawali, N. Molecular evolution of 5S rDNA region in Vigna subgenus Ceratotropis and its phylogenetic implications. Plant Syst Evol 280, 187–206 (2009). https://doi.org/10.1007/s00606-009-0178-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00606-009-0178-4

Keywords

Search

Navigation