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Optimization of dark fermentative biohydrogen production from rice starch by Enterobacter aerogenes MTCC 2822 and Clostridium acetobutylicum MTCC 11274

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Abstract

Dark fermentative biohydrogen production (DFBHP) has potential for utilization of rice starch wastewater (RSWW) as substrate. The hydrogen production of Enterobacter aerogenes MTCC 2822 and Clostridium acetobutylicum MTCC 11274, in pure culture and co-culture modes, was evaluated. The experiments were performed in a 2 L bioreactor, for a batch time of 120 h. The co-culture system resulted in highest cumulative hydrogen (1.13 L H2/L media) and highest yield (1.67 mol H2/mol glucose). Two parameters were optimized through response surface methodology (RSM)—substrate concentration (3.0–5.0 g/L) and initial pH (5.5–7.5), in a three-level factorial design. A total of 11 runs were performed in duplicate, which revealed that 4.0 g/L substrate concentration and 6.5 initial pH were optimal in producing hydrogen. The metabolites produced were acetic, butyric, propionic, lactic and isobutyric acids. The volumetric H2 productions, without and with pH adjustments, were 1.24 L H2/L media and 1.45 L H2/L media, respectively.

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Abbreviations

DFBHP:

Dark fermentative biohydrogen production

RSWW:

Rice starch wastewater

RSM:

Response surface methodology

CCD:

Central composite design

COD:

Chemical oxygen demand

VHPR:

Volumetric hydrogen production rate

HY:

Hydrogen yield

TDS:

Total dissolved solids

TSS:

Total suspended solids

FS:

Fixed solids

VS:

Volatile solids

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Acknowledgements

The authors are thankful to the Ministry of Education (MoE), Government of India, for providing post-graduation fellowship to the first author. The authors are extremely grateful to the facilities and support provided by the Department of Biotechnology, Dr. B R Ambedkar National Institute of Technology, Jalandhar, India, for the conduct of experiments and completion of this work.

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  1. Department of Biotechnology, Dr. B R Ambedkar National Institute of Technology, Jalandhar, Punjab, 144027, India

    Varsha Jayachandran & Nitai Basak

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Conceptualization, Investigation, Validation of results and Writing—original draft: VJ. Conceptualization, Validation of results and interpreted data, Writing—review and editing, and overall supervision of study: NB. All authors have reviewed and approved the final version of the manuscript for submission.

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Correspondence to Nitai Basak.

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Jayachandran, V., Basak, N. Optimization of dark fermentative biohydrogen production from rice starch by Enterobacter aerogenes MTCC 2822 and Clostridium acetobutylicum MTCC 11274. Bioprocess Biosyst Eng 46, 535–553 (2023). https://doi.org/10.1007/s00449-022-02838-1

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