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Optimization of process parameters for enhanced biohydrogen production using potato waste as substrate by combined dark and photo fermentation

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Abstract

Potato waste, which contains starch as a primary carbohydrate, is generated domestically and industrially as solid or liquid waste. Biological fermentation process is a cost-effective and clean way of H2 production. The combined dark and photo fermentation technique is an economical and time-saving process with a higher H2 production rate and yield. The current study highlights the feasibility of using potato waste as substrate for H2 production in combined dark and photo fermentation. The dark and photo fermentative bacteria Enterobacter aerogenes MTCC2822 and Rhodobacter sphaeroides O.U.001 respectively were used at a ratio of 1:7 (w/w) for H2 production. Response surface methodology (RSM) with central composite design (CCD) was employed to optimize carbohydrate concentration, FeCl3 concentration, and pH for enhancing H2 production. During fermentation, pH drop inside photo bioreactor (PBR) was observed due to accumulation of volatile fatty acids (VFAs). The maximum H2 production was 1580 mL H2/L media at 2 g/L carbohydrate concentration and 0.2 g/L FeCl3 concentration at pH 7. In total, 53% of the initial carbohydrate was converted to hydrogen with H2 production yield of 6.31 mol H2/mol carbohydrate.

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Acknowledgements

The first author Satya Ranjan Das wishes to thank Ministry of Education, Government of India, for providing the fellowships. The authors are thankful to the Department of Biotechnology, Dr. B R Ambedkar National Institute of Technology Jalandhar (India), for providing facilities to carry out the research.

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  1. Department of Biotechnology, Dr. B. R. Ambedkar, National Institute of Technology, 144011, Jalandhar, Punjab, India

    Satya Ranjan Das & Nitai Basak

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Highlights

• Single-stage combined dark and photo fermentation was carried out using potato waste as feedstock in a custom-made 2 L photo bioreactor.

• Optimization was carried out by a central composite design matrix to study the interactive effect of the independent variable on response.

• The optimum value for carbohydrate and FeCl3 concentration was found to be 2 g/L and 0.2 g/L at pH 7.

• Maximum H2 production was 1580 mL H2/L media.

• Fifty-three percent of the initial carbohydrate was converted to hydrogen with yield of 6.31 mol H2/mol carbohydrate.

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Das, S.R., Basak, N. Optimization of process parameters for enhanced biohydrogen production using potato waste as substrate by combined dark and photo fermentation. Biomass Conv. Bioref. 14, 4791–4811 (2024). https://doi.org/10.1007/s13399-022-02588-w

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