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Removal of pigments from molasses wastewater by combining micro-electrolysis with biological treatment method

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Abstract

Pigments in molasses wastewater (MWW) effluent, such as melanoidins, were considered as kinds of the most recalcitrant and hazardous colorant contaminants to the environment. In this study, de-coloring the MWW by a synergistic combination of micro-electrolysis with bio-treatment was performed. Aiming to a high de-colorization yield, levels of nutrition source supplies, MWW dilution ratio, and micro-electrolysis reaction time were optimized accordingly. For a diluted (50 %, v/v) MWW, an maximum overall de-colorization yield (97.1 ± 0.5 %, for absorbance at 475 nm) was achieved through the bio-electrolysis treatment. In electrolysis bio-treatment, the positive effect of micro-electrolysis was also revealed by a promoted growth of fungal biomass as well as activities of ligninolytic enzymes. Activities of lignin peroxidase, manganese peroxidase, and laccase were promoted by 111.2, 103.9, and 7.7 %, respectively. This study also implied that the bio-treatment and the micro-electrolysis had different efficiencies on removal of pigments with distinct polarities.

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References

  1. Ganesh R, Rajinikanth R (2010) Anaerobic treatment of winery wastewater in fixed bed reactors. Bioproc Biosyst Eng 33:619–628

    Article  CAS  Google Scholar 

  2. Pant D, Adholeya A (2007) Biological approaches for treatment of distillery wastewater: a review. Bioresour Technol 98:2321–2334

    Article  CAS  Google Scholar 

  3. Satyawali Y, Balakrishnan M (2008) Wastewater treatment in molasses-based alcohol distilleries for COD and color removal: a review. J Environ Manag 86:481–497

    Article  CAS  Google Scholar 

  4. Arimi MM, Zhang Y (2014) Antimicrobial colorants in molasses distillery wastewater and their removal technologies. Int Biodeter Biodegr 87:34–43

    Article  CAS  Google Scholar 

  5. Fan L, Nguyen T (2011) Characterisation of the impact of coagulation and anaerobic bio-treatment on the removal of chromophores from molasses wastewater. Water Res 45:3933–3940

    Article  CAS  Google Scholar 

  6. Yadav S, Chandra R (2011) Characterization of potential MnP producing bacteria and its metabolic products during decolourisation of synthetic melanoidins due to biostimulatory effect of d-xylose at stationary phase. Process Biochem 46:1774–1784

    Article  CAS  Google Scholar 

  7. Sirianuntapiboon S, Zohsalam P (2004) De-colorization of molasses wastewater by Citeromyces sp. WR-43-6. Process Biochem 39:917–924

    Article  CAS  Google Scholar 

  8. González T, Terrón MC, Yagüe S, Zapico E, Galletti GC, González AE (2000) Pyrolysis/gas chromatography/mass spectrometry monitoring of fungal- biotreated distillery wastewater using Trametes sp. I-62 (CECT 20197). Rapid Commun Mass Spectrom 14:1417–1424

    Article  Google Scholar 

  9. Dhillon GS, Brar SK (2011) Bioproduction of hydrolytic enzymes using apple pomace waste by A. Niger: applications in biocontrol formulations and hydrolysis of chitin/chitosan. Bioproc Biosyst Eng 34:1017–1026

    Article  CAS  Google Scholar 

  10. Abrunhosa L, Oliveira F (2013) Lipase production by Aspergillus ibericus using olive mill wastewater. Bioproc Biosyst Eng 36:285–291

    Article  CAS  Google Scholar 

  11. Chavan MN, Dandi ND (2013) Bio-treatment of melanoidin-containing distillery spent wash effluent by free and immobilized Aspergillus oryzae MTCC 7691. Water Air Soil Pollut 224:1–10

    Article  CAS  Google Scholar 

  12. Shayegan J, Pazouki M (2005) Continuous de-colorization of anaerobically digested distillery wastewater. Process Biochem 40:1323–1329

    Article  CAS  Google Scholar 

  13. Ying D, Peng J (2012) Treatment of mature landfill leachate by internal micro-electrolysis integrated with coagulation: a comparative study on a novel sequencing batch reactor based on zero valent iron. J Hazard Mater 229–230:426–433

    Article  Google Scholar 

  14. Zhu Q, Guo S (2014) Stability of Fe–C micro-electrolysis and biological process in treating ultra-high concentration organic wastewater. Chem Eng J 255:535–540

    Article  CAS  Google Scholar 

  15. Cheng H, Xu W (2007) Pre-treatment of wastewater from triazine manufacturing by coagulation, electrolysis, and internal microelectrolysis. J Hazard Mater 146:385–392

    Article  CAS  Google Scholar 

  16. Shi Y, Liu H (2009) Mechanism on impact of internal-electrolysis pre-treatment on biodegradability of yeast wastewater. Chin Sci Bull 54:2124–2130

    CAS  Google Scholar 

  17. Ruan X, Liu M (2010) Degradation and de-colorization of reactive red X-3B aqueous solution by ozone integrated with internal micro-electrolysis. Sep Purif Technol 74:195–201

    Article  CAS  Google Scholar 

  18. Ong S, Toorisaka E, Hirata M, Hano T (2008) Combination of adsorption and biodegradation processes for textile effluent treatment using a granular activated carbon-bioflm confgured packed column system. J Environ Sci 20:952–956

    Article  CAS  Google Scholar 

  19. Wang L, Yao Y, Zhang Z, Sun L, Lu W, Chen W, Chen H (2014) Activated carbon fibers as an excellent partner of Fenton catalyst for dyes decolorization by combination of adsorption and oxidation. Chem Eng J 251:348–354

    Article  CAS  Google Scholar 

  20. Imran B, Khan SJ, Qazi IA, Arshad M (2016) Removal and recovery of sodium hydroxide (NaOH) from industrial wastewater by two-stage diffusion dialysis (DD) and electrodialysis (ED) processes. Desalin Water Treat 57:7926–7932

    Article  CAS  Google Scholar 

  21. Ntampou X, Zouboulis AI, Samaras P (2006) Appropriate combination of physico-chemical methods (coagulation/flocculation and ozonation) for the efficient treatment of landfill leachates. Chemosphere 62:722–730

    Article  CAS  Google Scholar 

  22. Lo TC, Chang CA (2011) Correlation evaluation of antioxidant properties on the monosaccharide components and glycosyl linkages of polysaccharide with different measuring methods. Carbohydr Polym 86:320–327

    Article  CAS  Google Scholar 

  23. Pant D, Adholeya A (2007) Enhanced production of ligninolytic enzymes and de-colorization of molasses distillery wastewater by fungi under solid-state fermentation. Biodegradation 18:647–659

    Article  CAS  Google Scholar 

  24. Patil PU, Kapadnis BP (2003) Decolorisation of synthetic melanoidin and biogas effluent by immobilised fungal isolate of Aspergillus niger UM2. Int Sugar J 105(8):10–13

    Google Scholar 

  25. Miranda MP, Benito GG (1996) Color elimination from molasses wastewater by Aspergillus niger. Bioresour Technol 57:229–235

    Article  Google Scholar 

  26. Mikiashvili N, Wasser SP (2006) Effects of carbon and nitrogen sources on Pleurotus ostreatus ligninolytic enzyme activity. World J Microbiol Biotechnol 22:999–1002

    Article  CAS  Google Scholar 

  27. Ohmomo S, Kainuma M (1988) Adsorption of Melanoidin to the Mycelia of Aspergillus oryzae Y-2-32 (Microbiology & Fermentation Industry). Agric Biol Chem 52:381–386

    CAS  Google Scholar 

  28. Zhang X, Dong W et al (2014) Degradation efficiency and mechanism of azo dye RR2 by a novel ozone aerated internal micro-electrolysis filter. J Hazard Mater 276:77–87

    Article  CAS  Google Scholar 

  29. Zhou H, Lv P (2013) Identification of degradation products of ionic liquids in an ultrasound assisted zero-valent iron activated carbon micro-electrolysis system and their degradation mechanism. Water Res 47:3514–3522

    Article  CAS  Google Scholar 

  30. Zhou X, Liu H, Wang YX (2006) Internal electrolysis treatment of yeast-producing wastewater (in Chinese). Environ Sci Technol 29:69–71

    CAS  Google Scholar 

  31. Do JY, Salunkhe DK (1975) Water quality: food processing and health. Crit Rev Food Sci Nutr 5:281–298

    CAS  Google Scholar 

  32. Moilanen U, Winquist E (2015) Production of manganese peroxidase and laccase in a solid-state bioreactor and modeling of enzyme production kinetics. Bioproc Biosyst Eng 38:57–68

    Article  CAS  Google Scholar 

  33. Hadibarata T, Nor NM (2014) De-colorization and degradation mechanism of Amaranth by Polyporus sp. S133. Bioproc Biosyst Eng 37:1879–1885

    Article  CAS  Google Scholar 

  34. Yoshio Watanabe RSY (1982) Enzymatic de-colorization of Melanoidin sp. No. 20. Agric Biol Chem 6:1623–1630

    Google Scholar 

  35. Ergul FE, Sargin S (2009) Dephenolisation of olive mill wastewater using adapted Trametes versicolor. Int Biodeter Biodegr 63:1–6

    Article  CAS  Google Scholar 

  36. Asses N, Ayed L (2009) Biodegradation of different molecular-mass polyphenols derived from olive mill wastewaters by Geotrichum candidum. Int Biodeter Biodegr 63:407–413

    Article  CAS  Google Scholar 

  37. Vahabzadeh F, Mehranian M (2004) Color removal ability of Phanerochaete chrysosporium in relation to lignin peroxidase and manganese peroxidase produced in molasses wastewater. World J Microbiol Biotechnol 20:859–864

    Article  CAS  Google Scholar 

  38. Yadav S, Chandra R (2012) Biodegradation of organic compounds of molasses melanoidin (MM) from biomethanated distillery spent wash (BMDS) during the decolourisation by a potential bacterial consortium. Biodegradation 23:609–620

    Article  CAS  Google Scholar 

  39. Bharagava RN, Chandra R (2009) Isolation and characterization of aerobic bacteria capable of the degradation of synthetic and natural melanoidins from distillery effluent. World J Microbiol Biotechnol 25:737–744

    Article  CAS  Google Scholar 

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Acknowledgments

The authors acknowledge the financial support from National Natural Science Foundation of China (Grant Numbers: 51338010, 21107125 and 51221892).

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Authors and Affiliations

  1. School of Bioscience and Bioengineering, South China University of Technology, Guangzhou, 510006, China

    Ben Chen, Xiaofei Tian & Zhenqiang Wu

  2. School of Environment and Energy, South China University of Technology, Guangzhou, 510006, China

    Lian Yu

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  1. Ben Chen
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  2. Xiaofei Tian
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  3. Lian Yu
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  4. Zhenqiang Wu
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Correspondence to Zhenqiang Wu.

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The authors declare that they have no conflict of Interest in the research.

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X. Tian co-first author.

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Chen, B., Tian, X., Yu, L. et al. Removal of pigments from molasses wastewater by combining micro-electrolysis with biological treatment method. Bioprocess Biosyst Eng 39, 1867–1875 (2016). https://doi.org/10.1007/s00449-016-1661-2

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  • DOI: https://doi.org/10.1007/s00449-016-1661-2

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