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Isolation and characterization of aerobic bacteria capable of the degradation of synthetic and natural melanoidins from distillery effluent

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Abstract

Melanoidins, complex biopolymer of amino-carbonyl compounds are the major coloring and polluting constituents of distillery wastewaters. In this study, three aerobic melanoidin-degrading bacteria (RNBS1, RNBS3 and RNBS4) were isolated from soil contaminated with distillery effluent and characterized as Bacillus licheniformis (RNBS1), Bacillus sp. (RNBS3) and Alcaligenes sp. (RNBS4) by biochemical tests and 16S rRNA gene sequence analysis. The degradation of synthetic and natural melanoidins was studied by using the axenic and mixed bacterial consortium. Results have revealed that the mixed consortium was more effective compared to axenic culture decolorizing 73.79 and 69.83% synthetic and natural melanoidins whereas axenic cultures RNBS1, RNBS3 and RNBS4 decolorized 65.88, 62.56 and 66.10% synthetic and 52.69, 48.92 and 59.64% natural melanoidins, respectively. The HPLC analysis of degraded samples has shown reduction in peak areas compared to controls, suggesting that decrease in color intensity might be largely attributed to the degradation of melanoidins by isolated bacteria.

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Acknowledgments

The financial assistance received by Mr. R. N. Bharagava, SRF from University Grants Commission and CSIR Network project (NWP-19), New Delhi is gratefully acknowledged.

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Authors and Affiliations

  1. Environmental Microbiology Section, Indian Institute of Toxicology Research (CSIR), P.O. Box 80, M.G. Marg, Lucknow, Uttar Pradesh, 226001, India

    Ram Naresh Bharagava & Ram Chandra

  2. School of Studies in Life Sciences, Pt. Ravishankar Shukla University, Raipur, Chhattisgarh, 492010, India

    Vibhuti Rai

Authors
  1. Ram Naresh Bharagava
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  2. Ram Chandra
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  3. Vibhuti Rai
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Correspondence to Ram Chandra.

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Bharagava, R.N., Chandra, R. & Rai, V. Isolation and characterization of aerobic bacteria capable of the degradation of synthetic and natural melanoidins from distillery effluent. World J Microbiol Biotechnol 25, 737–744 (2009). https://doi.org/10.1007/s11274-008-9944-7

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  • DOI: https://doi.org/10.1007/s11274-008-9944-7

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