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Functional analysis of 5′ untranslated region of a TIR-NBS-encoding gene from triploid white poplar

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Abstract

Genome-wide analyses have identified a set of TIR-NBS-encoding genes in plants. However, the molecular mechanism underlying the expression of these genes is still unknown. In this study, we presented a TIR-NBS-encoding gene, PtDrl02, that displayed a low level of tissue-specific expression in a triploid white poplar [(Populus tomentosa × P. bolleana) × P. tomentosa], and analyzed the effects of the 5′ untranslated region (UTR) on gene expression. The 5′ UTR sequence repressed the reporter activity of β-glucuronidase (GUS) gene under PtDrl02 promoter by 113.5-fold with a staining ratio of 2.97% in the transgenic tobacco plants. Quantitative RT-PCR assays revealed that the 5′ UTR sequence decreased the transcript level of the GUS reporter gene by 13.3-fold, implying a regulatory role of 5′ UTR in transcription and/or mRNA destabilization. The comparison of GUS activity with the transcript abundance indicated that the 5′ UTR sequence decreased the translation efficiency of target gene by 88.3%. Additionally, the analysis of the transgenic P-985/UTRΔ/GUS plants showed that both the exon1 sequence and the leading intron within the 5′ UTR region were responsible for the regulation of gene expression. Our results suggested a negative effect of the 5′ UTR of PtDrl02 gene on gene expression.

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Acknowledgments

This research was supported by the National Natural Sciences Foundation of China (No. 30872043), the National TCM Project Application in the 11th Five-Year Plan in China (No. 2006BAD01A15-2) and the Doctor Foundation of Ministry of Education of China (No. 20070022003). We thank Dr. Wan Hong Yang from Nanyang Technological University in Singapore for paper revision.

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  1. National Engineering Laboratory for Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, Beijing Forestry University, 100083, Beijing, People’s Republic of China

    Huiquan Zheng, Shanzhi Lin, Qian Zhang, Yang Lei & Zhiyi Zhang

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  1. Huiquan Zheng
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Correspondence to Zhiyi Zhang.

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Communicated by R. Hagemann.

H. Zheng and S. Lin contributed equally to this work.

The GenBank accession numbers for the triploid white poplar PtDrl02 genomic and promoter sequences are DQ324361 and EF424611, respectively.

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Zheng, H., Lin, S., Zhang, Q. et al. Functional analysis of 5′ untranslated region of a TIR-NBS-encoding gene from triploid white poplar. Mol Genet Genomics 282, 381–394 (2009). https://doi.org/10.1007/s00438-009-0471-5

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