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Annona muricata leaf extract-mediated silver nanoparticles synthesis and its larvicidal potential against dengue, malaria and filariasis vector

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Abstract

Mosquitoes transmit several diseases which cause millions of deaths every year. The use of synthetic insecticides to control mosquitoes caused diverse effects to the environment, mammals, and high manufacturing cost. The present study was aimed to test the larvicidal activity of green synthesized silver nanoparticles using Annona muricata plant leaf extract against third instar larvae of three medically important mosquitoes, i.e., Aedes aegypti, Anopheles stephensi, and Culex quinquefasciatus. The different concentrations of green synthesized Ag Nanoparticles (AgNPs; 6, 12, 18, 24, 30 μg mL−1) and aqueous crude leaf extract (30, 60, 90, 120, 150 μg mL−1) were tested against the larvae for 24 h. Significant larval mortality was observed after the treatment of A. muricata for all mosquitoes with lowest LC50 and LC90 values, viz., A. aegypti (LC50 and LC90 values of 12.58 and 26.46 μg mL−1), A. stephensi (LC50 and LC90 values of 15.28 and 31.91 μg mL−1) and C. quinquefasciatus (LC50 and LC90 values of 18.77 and 35.72 μg mL−1), respectively. The synthesized AgNPs from A. muricata were highly toxic than aqueous crude extract. The nanoparticle characterization was done using spectral and microscopic analysis, namely UV-visible spectroscopy which showed a sharp peak at 420 nm of aqueous medium containing AgNPs, X-ray diffraction (XRD) analysis revealed the average crystalline size of synthesized AgNPs (approximately 45 nm), and Fourier transform infrared spectroscopy (FTIR) study exhibited prominent peaks 3381.28, 2921.03, 1640.17, 1384.58, 1075.83, and 610.77 cm−1. Particle size analysis (PSA) showed the size and distribution of AgNPs (103 nm); field emission scanning electron microscopy (FE-SEM) and high-resolution transmission electron microscopy (HR-TEM) analysis showed a spherical shape, size range from 20 to 53 nm; and energy-dispersive X-ray spectroscopy (EDX) reflects the chemical composition of synthesized AgNPs. Heat stability of the AgNPs was confirmed between the temperatures 20 to 70 °C. The result suggests that green synthesized AgNPs from A. muricata has the potential to be used as a low-cost and eco-friendly approach for the control of selected mosquitoes.

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Acknowledgments

We thank the Department of Biotechnology, Periyar University, Salem, India for the laboratory facilities provided. Authors are thankful to the DST Unit of Nanoscience, IIT Madras for the TEM characterization. We extend our thanks to CIT, Coimbatore for the SEM analysis. The authors would also like to thank the Department of Physics, Periyar University for the XRD and FTIR characterization analysis.

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  1. Natural Drug Research Laboratory, Department of Biotechnology, School of Biosciences, Periyar University, Salem, 636 011, Tamil Nadu, India

    S. B. Santhosh, R. Yuvarajan & D. Natarajan

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  1. S. B. Santhosh
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  2. R. Yuvarajan
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  3. D. Natarajan
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Correspondence to D. Natarajan.

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Santhosh, S.B., Yuvarajan, R. & Natarajan, D. Annona muricata leaf extract-mediated silver nanoparticles synthesis and its larvicidal potential against dengue, malaria and filariasis vector. Parasitol Res 114, 3087–3096 (2015). https://doi.org/10.1007/s00436-015-4511-2

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