Skip to main content
SpringerLink
Log in

Oviposition-deterrent, ovicidal, and repellent activities of indigenous plant extracts against Anopheles subpictus Grassi (Diptera: Culicidae)

  • Original Paper
  • Published:
Parasitology Research Aims and scope Submit manuscript

Abstract

Insecticides of botanical origin may serve as suitable alternative biocontrol techniques in the future. The leaf acetone, ethyl acetate, and methanol extracts of Aegle marmelos (Linn.) Correa ex Roxb, Andrographis lineata Wallich ex Nees, and Cocculus hirsutus (L.) Diels were tested for oviposition-deterrent, ovicidal, and repellent activities against Anopheles subpictus Grassi (Diptera: Culicidae). The percentage of effective oviposition repellency of 92.60 , 93.04, 95.20, 88.26, 92.80, 94.01, 95.77, 96.93, and 92.54 at 500 ppm and the lowest repellency of 47.14, 58.00, 56.52, 64.93, 71.09, 66.42, 50.62, 57.62, and 65.73 at 31.25 ppm in acetone, ethyl acetate, and methanol extracts of Aegle marmelos, Andrographis lineata, and Cocculus hirsutus, respectively. The oviposition activity index (OAI) value of acetone, ethyl acetate, and methanol extracts of Aegle marmelos, Andrographis lineata, and Cocculus hirsutus at 500 ppm were −0.86, −0.87, −0.90, −0.78, −0.87, −0.86, −0.91, −0.94, and −0.86 respectively. The OAI values revealed that the solvent plant extracts have deterrent effect, and they caused a remarkable negative response resulting in oviposition of very few eggs. Mean percent hatchability of the ovicidal activity was observed 24 h after treatment. The percent hatchability was inversely proportional to the concentration of extract and directly proportional to the eggs. Mortality of 100% with ethyl acetate extract of Aegle marmelos, methanol extracts Aegle marmelos, Andrographis lineata, and Cocculus hirsutus were exerted at 1,000 ppm. The maximum repellent activity was observed at 500 ppm in methanol extracts of Aegle marmelos, Andrographis lineata, and ethyl acetate extract of Cocculus hirsutus, and the mean complete protection time ranged from 90 to 120 min with the different extracts tested. These results suggest that the leaf extracts of Aegle marmelos, Andrographis lineata, and Cocculus hirsutus have the potential to be used as an ideal ecofriendly approach for the control of the Anopheles subpictus. Therefore, this study provides first report on the oviposition, ovicidal, and repellent activities against malaria vector, Anopheles subpictus of plant extracts from Southern India.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Al-Doghairi M, El-Nadi A, Elhag E, Al-Ayedh H (2004) Effect of Solenostemma argel on oviposition, egg hatchability and viability of Culex pipiens L. larvae. Phytother Res 18(4):335–338

    Article  PubMed  Google Scholar 

  • Amer A, Mehlhorn H (2006) Repellency effect of forty-one essential oils against Aedes, Anopheles, and Culex mosquitoes. Parasitol Res 99(4):478–490

    Article  PubMed  Google Scholar 

  • Amerasinghe PH, Amerasinghe FP (1999) Multiple host feeding in field populations of Anopheles culicifacies and A. subpictus in Sri Lanka. Med Vet Entomol 13(2):124–131

    Article  CAS  PubMed  Google Scholar 

  • Autran ES, Neves IA, da Silva CS, Santos GK, da Câmara CA, Navarro DM (2009) Chemical composition, oviposition deterrent and larvicidal activities against Aedes aegypti of essential oils from Piper marginatum Jacq. (Piperaceae). Bioresour Technol 100(7):2284–2288

    Article  CAS  PubMed  Google Scholar 

  • Banerji N, Kumar R (1980) Studies on the seed oil of Aegle marmelos and its effect on some bacterial species. J Inst Chem 52(II):59–60

    CAS  Google Scholar 

  • Breman JG, Martin AS, Mills A (2004) Conquering the intolerable burden of malaria: what’s new, what’s needed: a summary. Am J Trop Med Hyg 71(2):1–15

    PubMed  Google Scholar 

  • Caius JF (1986) The medicinal and poisonous plants of India. Scientific Publishers, Jodhpur, pp 166–171

    Google Scholar 

  • Chenniappan K, Kadarkarai M (2008) Oviposition deterrent, ovicidal and gravid mortality effects of ethanolic extract of Andrographis paniculata Nees against the malarial vector Anopheles stephensi Liston (Diptera: Culicidae). Entomological Research 38:119–125

    Article  Google Scholar 

  • Coria C, Almiron W, Valladares G, Carpinella C, Ludueña F, Defago M, Palacios S (2008) Larvicide and oviposition deterrent effects of fruit and leaf extracts from Melia azedarach L. on Aedes aegypti (L.) (Diptera: Culicidae). Bioresour Technol 99(8):3066–3070

    Article  CAS  PubMed  Google Scholar 

  • Elango G, Rahuman AA, Bagavan A, Kamaraj C, Zahir AA, Venkatesan C (2009) Laboratory study on larvicidal activity of indigenous plant extracts against Anopheles subpictus and Culex tritaeniorhynchus. Parasitol Res 104(6):1381–1388

    Article  CAS  PubMed  Google Scholar 

  • Fatope MO, Ibrahim H, Takeda Y (1993) Screening of higher plants reputed as pesticides using the brine shrimp lethality assay. Int J Pharmacognosy 31:250–254

    Article  Google Scholar 

  • Fradin MS, Day JF (2002) Comparative efficacy of insect repellents against mosquito bite. New Engl J Med 347:13–18

    Article  CAS  PubMed  Google Scholar 

  • Ganapaty S, Dash GK, Subburaju T, Suresh P (2002) Diuretic, laxative and toxicity studies of Cocculus hirsutus aerial parts. Fitoterapia 73(1):28–31

    Article  CAS  PubMed  Google Scholar 

  • Geetha I, Pailey P, Padmanaban V, Balaraman K (2003) Oviposition, response of the mosquito, Culex quinquefasciatus to the secondary metabolite(s) of the fungus, Trichoderma viridae. Mem Inst Oswaldo Cruz 98:223–226

    Article  CAS  PubMed  Google Scholar 

  • Govindachari TR, Suresh G, Krishna Kumari GN, Rajamannar T, Partho PD (1999) Nymania-3. A bioactive triterpenoid from Dysoxylum malabaricum. Fitoterapia 70:83–86

    Article  CAS  Google Scholar 

  • Govindarajan M (2009) Bioefficacy of Cassia fistula Linn. (Leguminosae) leaf extract against chikungunya vector, Aedes aegypti (Diptera: Culicidae). Eur Rev Med Pharmacol Sci 13(2):99-103

  • Govindarajan M, Jebanesan A, Pushpanathan T (2008b) Larvicidal and ovicidal activity of Cassia fistula Linn. leaf extract against filarial and malarial vector mosquitoes. Parasitol Res 102(2):289-292

    Google Scholar 

  • Govindarajan M, Jebanesan A, Pushpanathan T, Samidurai K (2008a) Studies on effect of Acalypha indica L. (Euphorbiaceae) leaf extracts on the malarial vector, Anopheles stephensi Liston (Diptera:Culicidae). Parasitol Res 103(3):691-695

    Google Scholar 

  • Hebbalkar DS, Sharma RN, Joshi VS, Bhat VS (1992) Mosquito repellent activity of oils from Vitex negundo Linn. Leaves Indian J Med Res 95:200–203

    CAS  Google Scholar 

  • Hisham A, Ajitha Bai MD, Jayakumar G, Nair MS, Fujimoto Y (2001) Triterpenoids from Dysoxylum malabaricum. Phytochemistry 56:331–334

    Article  CAS  PubMed  Google Scholar 

  • Hoedojo PF, Atmosoedjono S, Purnomo TT (1980) A study on vectors of Bancroftian filariasis in West Flores, Indonesia. Southeast Asian J Trop Med Public Health 11(3):399–404

    CAS  PubMed  Google Scholar 

  • Jaenson TG, Pålsson K, Borg-Karlson AK (2006) Evaluation of extracts and oils of mosquito (Diptera: Culicidae) repellent plants from Sweden and Guinea-Bissau. J Med Entomol 43(1):113–119

    Article  CAS  PubMed  Google Scholar 

  • Jantan I, Yalvema MF, Ahmad NW, Jamal JA (2005) Insecticidal activities of the leaf oils of eight Cinnamomum species against Aedes aegypti and Aedes albopictus. Pharmaceutical Biol 43:526–532

    Article  CAS  Google Scholar 

  • Jeyabalan D, Arul N, Thangamathi P (2003) Studies on effects of Pelargonium citrosa leaf extracts on malarial vector, Anopheles stephensi Liston. Bioresour Technol 89(2):185–189

    Article  CAS  PubMed  Google Scholar 

  • Kamaraj C, Bagavan A, Rahuman AA, Zahir AA, Elango G, Pandiyan G (2008) Larvicidal potential of medicinal plant extracts against Anopheles subpictus Grassi and Culex tritaeniorhynchus Giles (Diptera: Culicidae). Parasitol Res 104(5):1163–1171

    Article  PubMed  Google Scholar 

  • Karunamoorthi K, Ramanujam S, Rathinasamy R (2008) Evaluation of leaf extracts of Vitex negundo L. (Family: Verbenaceae) against larvae of Culex tritaeniorhynchus and repellent activity on adult vector mosquitoes.

  • Kim SW, Chang IM, Oh KB (2002) Inhibition of the bacterial surface protein anchoring transpeptidase sortase by medicinal plants. Biosci Biotechnol Biochem 66(12):2751–2754

    Article  CAS  PubMed  Google Scholar 

  • Kramer WL, Mulla S (1979) Oviposition attractants and repellents of mosquitoes: oviposition responses of Culex mosquitoes to organic infusions. Enviro Entomol 8:1111–1117

    Google Scholar 

  • Kumar A, Valecha N, Jain T, Aditya P (2007) Dash burden of malaria in India: retrospective and prospective view. Am J Trop Med Hyg 77(6):69–78

    PubMed  Google Scholar 

  • Kuppusamy C, Murugan K (2006) Mosquitocidal effect of ethanolic extracts of Andrographis paniculata Nees on filarial vector Culex quinqufasciatus Say (Diptera: Culicidae). In: International Conference on Diversity of Insects: Challenging Issues in Management and Conservation, 30 January–3 February 2006, Tamil Nadu, India pp. 194

  • Mehra BK, Hiradhar PK (2002) Cuscuta hyalina Roth., an insect development inhibitor against common house mosquito Culex quinquefasciatus Say. J Environ Biol 23:335–339

    PubMed  Google Scholar 

  • Millar JG, Chaney JD, Mulla MS (1992) Identification of oviposition attractants for Culex quinquefasciatus from fermented Bermuda grass infusion. J Am Mosq Control Assoc 8:11–17

    CAS  PubMed  Google Scholar 

  • Miura T, Schafer CH, Takahashi RM, Mulligan FS (1976) Effects of insect growth inhibitor, dimilin on hatching of mosquito eggs. J Econ Ent 69:655–658

    CAS  Google Scholar 

  • Moore CG (1977) Insecticide avaoidance by ovipositing Aedes aegypti. Mosquito News 7:291–293

    Google Scholar 

  • Mullai K, Jebanesan A, Pushpanathan T (2008) Effect of bioactive fractions of Citrullus vulgaris Schrad. leaf extract against Anopheles stephensi and Aedes aegypti. Parasitol Res 102 (5):951-955

  • Olagbemiro TO, Birkett MA, Mordue AJ, Pickett JA (1999) Production of (5-R, 6-S)-6-acetoxy-5-hexadecanolide, the mosquito oviposition pheromone, from the seed oil of the summer cypress plant, Kochia scoparia (Chenopodiaceae). J Agric Food Chem 47:3411–3415

    Article  CAS  PubMed  Google Scholar 

  • Ouda NAA, Al-chalabi BBM, FFMR Al- charchafchi, Mohsen ZZH (1998) Extract of Atriplex canescens against Culex quinquefasciatus. Pharm Biol 36(1):69–71

    Article  Google Scholar 

  • Pandey SK, Upadhyay S, Tripathi AK (2009) Insecticidal and repellent activities of thymol from the essential oil of Trachyspermum ammi (Linn) Sprague seeds against Anopheles stephensi. Parasitol Res 105(2):507–512

    Article  CAS  PubMed  Google Scholar 

  • Pitasawat B, Choochote W, Tuetun B, Tippawangkosol P, Kanjanapothi D, Jitpakdi A, Riyong D (2003) Repellency of aromatic turmeric Curcuma aromatica under laboratory and field conditions. J Vector Ecol 28(2):234–240

    PubMed  Google Scholar 

  • Prajapati V, Tripathi AK, Aggarwal KK, Khanuja SPS (2005) Insecticidal, repellent and oviposition-deterrent activity of selected essential oils against Anopheles stephensi, Aedes aegypti and Culex quinquefasciatus. Bioresour Technol 96:1749–1757

    Article  CAS  PubMed  Google Scholar 

  • Prakash A, Rao J (1997) Botanical pestcides in agriculture. CRC, Boca Raton, FL

    Google Scholar 

  • Pushpanathan T, Jebanesan A, Govindarajan M (2008) The essential oil of Zingiber officinalis Linn (Zingiberaceae) as a mosquito larvicidal and repellent agent against the filarial vector Culex quinquefasciatus Say (Diptera: Culicidae). Parasitol Res 102(6):1289–1291

    Article  PubMed  Google Scholar 

  • Rahuman AA, Gopalakrishnan G, Venkatesan P, Geetha K (2008) Isolation and identification of mosquito larvicidal compound from Abutilon indicum (Linn.) Sweet. Parasitol Res 102:981–988

    Article  PubMed  Google Scholar 

  • Rajkumar S, Jebanesan A (2005) Oviposition deterrent and skin repellent activities of Solanum trilobatum leaf extract against the malarial vector Anopheles stephensi. J Insect Sci 5:15

    CAS  PubMed  Google Scholar 

  • Rajkumar S, Jebanesan A (2009) Larvicidal and oviposition activity of Cassia obtusifolia Linn (Family: Leguminosae) leaf extract against malarial vector, Anopheles stephensi Liston (Diptera: Culicidae). Parasitol Res 104(2):337–340

    Article  CAS  PubMed  Google Scholar 

  • Rana BK, Singh UP, Taneja D (1997) Antifungal activity and kinetics of inhibition by essential oil isolated from leaves of Aegle marmelos. J Ethnopharmacol 57:29–34

    Article  CAS  PubMed  Google Scholar 

  • Renu (1983) Fungal toxicity of leaf extract of some higher plants against Rhizoctonia solani Luehm. Nat Acad Sci Lett 6:245–246

    Google Scholar 

  • Samarasekera JK, Khambay BP, Hemalal KP (2004) A new insecticidal protolimonoid from Aegle marmelos. Nat Prod Res 18(2):117–122

    Article  CAS  PubMed  Google Scholar 

  • Shoba FG, Thomas M (2001) Study of anti-diarrhoeal activity of four medicinal plants in castor oil induced diarrhoea. J Ethnopharmacol 76:73–76

    Article  CAS  PubMed  Google Scholar 

  • Singh RN, Saratchandra B (2005) The development of botanical products with special reference to seri-ecosystem. Caspian J Env Sci 3(1):1–8

    CAS  Google Scholar 

  • Snow RW, Guerra CA, Noor AM, Myint HY, Hay SI (2005) The global distribution of clinical episodes of Plasmodium falciparum Malaria. Nature 434(7030):214–217

    Article  CAS  PubMed  Google Scholar 

  • Sokal RR, Rohlf JF (1981) Biometry principles and practice of statistics in biological research, 2nd edn. WH Freeman and Company, New York

    Google Scholar 

  • Su T, Mulla MS (1998) Ovicidal activity of neem products (Azadirachtin) against Culex tarsalis and Culex quinquefasciatus (Diptera: Culicidae). J Am Mosq Cont Assoc 14:204–209

    CAS  Google Scholar 

  • Sukumar K, Perich MJ, Boobar LR (1991) Botanical derivatives in mosquito control: a review. J Am Mosq Control Assoc 72:210–237

    Google Scholar 

  • Tawatsin A, Asavadachanukorn P, Thavara U, Wongsinkongman P, Bansidhi J, Boonruad T, Chavalittumrong P, Soonthornchareonnon N, Komalamisra N, Mulla MS (2006) Repellency of essential oils extracted from plants in Thailand against four mosquito vectors (Diptera: Culicidae) and oviposition deterrent effects against Aedes aegypti (Diptera: Culicidae). Southeast Asian J Trop Med Public Health 37(5):915–931

    CAS  PubMed  Google Scholar 

  • Tripathi AK, Prajapati V, Ahmad A, Aggarwal KK, Khanuja SP (2004) Piperitenone oxide as toxic, repellent, and reproduction retardant toward malarial vector Anopheles stephensi (Diptera: Anophelinae). J Med Entomol 41(4):691–698

    Article  CAS  PubMed  Google Scholar 

  • Tyagi BK, Ramnath T, Shahi AK (1994) Evaluation of repellency effect of Tagetus minuta (Family: Compositae) against the vector mosquitoes Anopheles stephensi Liston. Culex quinquefasciatus Say and Aedes aegypti L. Int Pest Contr 39:48

    Google Scholar 

  • Usher G (1984) A dictionary of plants used by man, 1st edn. Delhi, CBS Publishers, p 163

    Google Scholar 

  • Venkatachalam MR, Jebanesan A (2001) Repellent activity of Ferronia elephantum Corr. (Rutaceae) leaf extract against Aedes aegypti (L.). Bioresour Technol76(3):287-288

  • Waliwitiya R, Kennedy CJ, Lowenberger CA (2009) Larvicidal and oviposition-altering activity of monoterpenoids, trans-anithole and rosemary oil to the yellow fever mosquito Aedes aegypti (Diptera: Culicidae). Pest Manag Sci 65(3):241–248

    Article  CAS  PubMed  Google Scholar 

  • Xue RD, Barnard DR, Ali A (2001) Laboratory and field evaluation of insect repellents as oviposition deterrents against mosquito Aedes albopictus. Med Vet Entomol 15:126–131

    Article  CAS  PubMed  Google Scholar 

  • Xue RD, Barnard DR, Ali A (2003) Laboratory evaluation of 18 repellent compounds as oviposition deterrents of Aedes albopictus and as larvicides of Aedes aegypti, Anopheles quadrimaculatus and Culex quinquefasciatus. J Am Mosq Contro l Assoc 19:397–403

    CAS  Google Scholar 

  • Xue RD, Barnard DR, Ali A (2006) Laboratory evaluation of 21 insect repellents as larvicides and as oviposition deterrents of Aedes albopictus (Diptera: Culicidae). J Am Mosq Control Assoc 22:126–130

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgements

The authors are grateful to C. Abdul Hakeem College Management, Dr. S. Mohammed Yousuff, Principal, Dr. K.Abdul Subhan, Reader and HOD of Zoology Department, and Dr. Sait Sahul Hameed, Reader in Zoology for their help and suggestion.

Author information

Authors and Affiliations

  1. Unit of Bioactive Natural Products, P.G & Research Department of Zoology, C. Abdul Hakeem College, Vellore District, Melvisharam, 632 509, Tamil Nadu, India

    G. Elango, A. Bagavan, C. Kamaraj, A. Abduz Zahir & A. Abdul Rahuman

Authors
  1. G. Elango
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. Bagavan
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. C. Kamaraj
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. A. Abduz Zahir
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. A. Abdul Rahuman
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to A. Abdul Rahuman.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Elango, G., Bagavan, A., Kamaraj, C. et al. Oviposition-deterrent, ovicidal, and repellent activities of indigenous plant extracts against Anopheles subpictus Grassi (Diptera: Culicidae). Parasitol Res 105, 1567–1576 (2009). https://doi.org/10.1007/s00436-009-1593-8

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00436-009-1593-8

Keywords

Search

Navigation