Abstract
Key message
OsICS1 but not OsICS1-L mediates the rice response to Xoo inoculation, with its overexpression increasing resistance against this pathogen. OsICS1 but not OsICS-L is directly upregulated by OsWRKY6.
Abstract
Rice (Oryza sativa) is a staple crop for about half of the global population and is particularly important in the diets of people living in Asia, Latin America, and Africa. This crop is continually threatened by bacterial leaf blight disease caused by Xanthomonas oryzae pv. oryzae (Xoo), which drastically reduces yields; therefore, it is needed to elucidate the plant’s resistance mechanisms against Xoo. Isochorismate synthase (ICS1) generates salicylic acid (SA) and increases resistance against bacterial disease. The OsICS1 is differently annotated in rice genome databases and has not yet been functionally characterized in the context of Xoo infection. Here, we report that the expression of the OsICS1 is directly regulated by OsWRKY6 and increases plant resistance against Xoo. Inoculation with Xoo increased the expression of OsICS1 but not that of the long variant of OsICS1 (OsICS1-L). OsWRKY6 directly activated the OsICS1 promoter but not the OsICS1-L promoter. OsICS1 overexpression in rice increased resistance against Xoo through the induction of SA-dependent bacterial defense genes. These data show that OsICS1 promotes resistance against Xoo infection.
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Data availability
The data and materials that support the findings of this study are openly available from the corresponding author K-HH upon reasonable request.
Abbreviations
- ChIP:
-
Chromatin immunoprecipitation
- GUS:
-
β-Glucuronidase
- ICS:
-
Isochorismate synthase
- SA:
-
Salicylic acid
- Xoo :
-
Xanthomonas oryzae Pv. oryzae
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Acknowledgements
This research was supported by the Brain Pool program funded by the Ministry of Science and ICT through the National Research Foundation of Korea (RS-2023-00262576) to J.I. This study was carried out with the support of the “Research Program for Agricultural Science & Technology Development (Project No. PJ01246303)”, National Institute of Agricultural Sciences, Rural Development Administration, Republic of Korea, and also supported by “Regional Innovation Strategy (RIS)” through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (MOE)(2023RIS-009).
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JI, CC, DH: conceptualization; JI, DH: funding acquisition; JI, MJ, CC: investigation; JI, CC, SP: methodology; DH: project administration; DH: supervision; JI, CC: visualization; JI, DH, MJ: writing and revision.
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425_2024_4405_MOESM1_ESM.pptx
Supplementary file1 Fig. S1 Primer sets for the RT-qPCR in OsICS1 and an epitope of the anti-OsICS1 antibody. Fig. S2 Expression of OsWRKY6 in three-week-old OsWRKY6 overexpressing (OsWRKY6OX) plants, as determined using RT-qPCR. Values are means ± SD (n= 3, **P < 0.01). Fig. S3 Protein blot analysis of OsICS1 in 3-week-old OsWRKY6-overexpressing (OsWRKY6OX) plants. The protein blot analysis was performed using an anti-OsICS1 antibody and OsWRKY6 antibody (Choi et al. 2015). Anti-histone 3 (H3) antibody was used for the loading control. Fig. S4 Disease incidence in OsICS1 overexpressing plants. OsICS1OX plants were inoculated at 9 weeks old with compatible XooKXO576 using the scissor-dip method. After 2 weeks, the image of the leaves was taken. Bar = 10 cm. (PPTX 661 KB)
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Im, J.H., Choi, C., Jung, MY. et al. The OsICS1 is directly regulated by OsWRKY6 and increases resistance against Xanthomonas oryzae pv. oryzae. Planta 259, 124 (2024). https://doi.org/10.1007/s00425-024-04405-2
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DOI: https://doi.org/10.1007/s00425-024-04405-2