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High frequency of HMW-GS sequence variation through somatic hybridization between Agropyron elongatum and common wheat

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Abstract

A symmetric somatic hybridization was performed to combine the protoplasts of tall wheatgrass (Agropyron elongatum) and bread wheat (Triticum aestivum). Fertile regenerants were obtained which were morphologically similar to tall wheatgrass, but which contained some introgression segments from wheat. An SDS-PAGE analysis showed that a number of non-parental high-molecular weight glutenin subunits (HMW-GS) were present in the symmetric somatic hybridization derivatives. These sequences were amplified, cloned and sequenced, to deliver 14 distinct HMW-GS coding sequences, eight of which were of the y-type (Hy1–Hy8) and six x-type (Hx1–Hx6). Five of the cloned HMW-GS sequences were successfully expressed in E. coli. The analysis of their deduced peptide sequences showed that they all possessed the typical HMW-GS primary structure. Sequence alignments indicated that Hx5 and Hy1 were probably derived from the tall wheatgrass genes Aex5 and Aey6, while Hy2, Hy3, Hx1 and Hy6 may have resulted from slippage in the replication of a related biparental gene. We found that both symmetric and asymmetric somatic hybridization could promote the emergence of novel alleles. We discussed the origination of allelic variation of HMW-GS genes in somatic hybridization, which might be the result from the response to genomic shock triggered by the merger and interaction of biparent genomes.

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Abbreviations

AFLP:

Amplification fragment length polymorphism

GISH:

Genome in situ hybridization

HMW-GS:

High-molecular weight glutenin subunit

SDS-PAGE:

Sodium dodecyl sulfate-polyacrylamide gel electrophoresis

References

  • Anderson OD, Greene FC (1989) The characterization and comparative analysis of high-molecular-weight glutenin genes from genomes A and B of a hexaploid bread wheat. Theor Appl Genet 77:689–700

    Article  CAS  Google Scholar 

  • Anderson OD, Greene FC, Yip RE, Halford NG, Shewry PR, Malpica-Romero J-M (1989) Nucleotide sequences of the two high-molecular-weight glutenin genes from the D-genome of a hexaploid bread wheat, Triticum aestivum L. cv Cheyenne. Nucleic Acids Res 17:461–462

    Article  CAS  PubMed  Google Scholar 

  • Branlard G, Dardevet M (1985) Diversity of grain protein and bread wheat quality. II. Correlation between high molecular weight subunits of glutenin and flour quality characteristics. J Cereal Sci 3:345–354

    Article  CAS  Google Scholar 

  • Cui H, Yu Z, Deng J, Gao X, Sun Y, Xia G (2009) Introgression of bread wheat chromatin into tall wheatgrass via somatic hybridization. Planta 229:323–330

    Article  CAS  PubMed  Google Scholar 

  • Feng DS, Xia GM, Zhao SY, Chen FG (2004) Two quality-associated HMW glutenin subunits in a somatic hybrid line between Triticum aestivum and Agropyron elongatum. Theor Appl Genet 110:136–144

    Article  CAS  PubMed  Google Scholar 

  • Forde J, Malpica J-M, Halford NG, Shewry PR, Anderson OD, Greene FC, Miflin BJ (1985) The nucleotide sequence of a HMW glutenin subunit gene located on chromosome 1A of wheat (Triticum aestivum L.). Nucleic Acids Res 13:6817–6832

    Article  CAS  PubMed  Google Scholar 

  • Halford NG, Forde J, Anderson OD, Greene FC, Shewry PR (1987) The nucleotide and deduced amino acid sequences of an HMW glutenin subunit gene from chromosome 1B of bread wheat (Triticum aestivum L.) and comparison with those of genes from chromosomes 1A and 1D. Theor Appl Genet 75:117–126

    Article  CAS  Google Scholar 

  • Halford NG, Field JM, Blair H, Urwin P, Moore K, Robert L, Thompson R, Flavell RB, Tatham AS, Shewry PR (1992) Analysis of HMW glutenin subunits encoded by chromosome 1A of bread wheat (Triticum aestivum L.) indicates quantitative effects on grain quality. Theor Appl Genet 83:373–378

    Article  CAS  Google Scholar 

  • Kumar S, Tamura K, Nei M (2004) MEGA3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5:150–163

    Article  CAS  PubMed  Google Scholar 

  • Lawrence GJ, Shepherd KW (1981) Inheritance of glutenin protein subunits of wheat. Theor Appl Genet 60:333–337

    Article  CAS  Google Scholar 

  • Liu SW, Zhao SY, Chen FG, Xia GM (2007) Generation of novel high quality HMW-GS genes in two introgression lines of Triticum aestivum/Agropyron elongatum. BMC Evol Biol 7:76

    Article  PubMed  Google Scholar 

  • Liu SW, Xin Gao, Xia GM (2008) Characterizing HMW-GS alleles of decaploid Agropyron elongatum in relation to evolution and wheat breeding. Theor Appl Genet 116:325–334

    Article  CAS  PubMed  Google Scholar 

  • Liu H, Liu SW, Xia GM (2009) Generation of high frequency of novel alleles of the high molecular weight glutenin in somatic hybridization between bread wheat and tall wheatgrass. Theor Appl Genet 118:1193–1198

    Article  CAS  PubMed  Google Scholar 

  • Mackie AM, Lagudah ES, Sharp PJ, Lafiandra D (1996) Molecular and biochemical characterization of HMW glutenin subunits from T. tauschii and the D genome of hexaploid wheat. J Cereal Sci 23:213–225

    Article  CAS  Google Scholar 

  • Madlung A, Masuelli RW, Watson B, Reynolds SH, Davison J, Comai L (2002) Remodeling of DNA methylation and phenotypic and transcriptional changes in synthetic Arabidopsis allotetraploids. Plant Physiol 129:733–746

    Article  CAS  PubMed  Google Scholar 

  • Madlung A, Tyagi AP, Watson B, Jiang H, Kagochi T, Doerge RW, Martienssen R, Comai L (2005) Genomic changes in synthetic Arabidopsis polyploids. Plant J 41:221–230

    Article  CAS  PubMed  Google Scholar 

  • McClintock B (1984) The significance of responses of the genome to challenge. Science 226:792–801

    Article  CAS  PubMed  Google Scholar 

  • Murray MG, Thompson WF (1980) Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res 8:4321–4325

    Article  CAS  PubMed  Google Scholar 

  • Ozkan H, Levy AA, Feldman M (2001) Allopolyploidy-induced rapid genome evolution in the wheat (Aegilops–Triticum) group. Plant Cell 13:1735–1747

    Article  CAS  PubMed  Google Scholar 

  • Payne PI (1987) Genetics of wheat storage proteins and the effect of allelic variation on breadmaking quality. Annu Rev Plant Physiol 38:141–153

    Article  CAS  Google Scholar 

  • Payne PI, Nightingale MA, Krattiger AF, Holt LM (1987) The relationship between HMW glutenin subunit composition and the breadmaking quality of British grown wheat varieties. J Sci Food Agric 40:51–65

    Article  CAS  Google Scholar 

  • Payne PI, Holt LM, Krattiger AF, Carrillo JM (1988) Relationship between seed quality characteristics and HMW glutenin subunit composition determined using wheats grown in Spain. J Cereal Sci 7:229–235

    Article  CAS  Google Scholar 

  • Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, New York

    Google Scholar 

  • Shaked H, Kashkush K, Ozkan H, Feldman M, Levy AA (2001) Sequence elimination and cytosine methylation are rapid and reproducible responses of the genome to wide hybridization and allopolyploidy in wheat. Plant Cell 13:1749–1759

    Article  CAS  PubMed  Google Scholar 

  • Shewry PR, Halford NG, Tatham AS, Popineau Y, Lafiandra D, Belton PS (2003) The high molecular weight subunits of wheat glutenin and their role in determining wheat processing properties. Adv Food Nut Res 45:219–302

    Article  CAS  Google Scholar 

  • Sugiyama T, Rafalski A, Peterson D, Söll D (1985) A wheat HMW glutenin subunit gene reveals a highly repeated structure. Nucleic Acids Res 13:8729–8737

    Article  CAS  PubMed  Google Scholar 

  • Thompson RD, Bartels D, Harberd NP (1985) Nucleotide sequence of a gene from chromosome 1D of wheat encoding a HMW-glutenin subunit. Nucleic Acids Res 13:6833–6846

    Article  CAS  PubMed  Google Scholar 

  • Wan Y, Wang D, Shewry PR, Halford NG (2002) Isolation and characterization of five novel high molecular weight subunit of glutenin genes from Triticum timopheevi and Aegilops cylindrica. Theor Appl Genet 104:828–839

    Article  CAS  PubMed  Google Scholar 

  • Wang J, Xiang FN, Xia GM (2005) Agropyron elongatum chromatin localization on the wheat chromosomes in an introgression line. Planta 211:277–286

    Article  Google Scholar 

  • Wells RD (1996) Molecular basis of genetic instability of triplet repeats. J Biol Chem 271:2875–2878

    CAS  PubMed  Google Scholar 

  • Xia GM, Xiang FN, Zhou AF, Wang H, Chen HM (2003) Asymmetric somatic hybridization between wheat (Triticum aestivum L.) and Agropyron elongatum (Host) Nevishi. Theor Appl Genet 107:299–305

    Article  CAS  PubMed  Google Scholar 

  • Zhou HP, Li B, Li ZS (1995) The study of breeding blue-grain gene translocation of wheat (in Chinese with English Abstract). Acta Bot Boreal-Occident Sin 15:125–128

    Google Scholar 

Download references

Acknowledgments

This research was supported by the National Natural Science Foundation of China No. 30871320 and National 863 High Technology Research and Development Project 2006AA100102, the National Key Technology R&D Program 2007BAD59B06 and the National Transgenic Project 2009ZX08002-014B.

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Authors and Affiliations

  1. The Key Laboratory of Plant Cell Engineering and Germplasm Innovation, Ministry of Education, School of Life Science, Shandong University, 250100, Jinan, People’s Republic of China

    Xin Gao, Shu Wei Liu, Qun Sun & Guang Min Xia

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  1. Xin Gao
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  2. Shu Wei Liu
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  3. Qun Sun
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  4. Guang Min Xia
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Correspondence to Guang Min Xia.

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X. Gao and S. W. Liu contributed equally to the work.

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Gao, X., Liu, S.W., Sun, Q. et al. High frequency of HMW-GS sequence variation through somatic hybridization between Agropyron elongatum and common wheat. Planta 231, 245–250 (2010). https://doi.org/10.1007/s00425-009-1040-1

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