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Production and characterization of a novel interspecific somatic hybrid combining drought tolerance and high quality of sweet potato and Ipomoea triloba L.

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Abstract

Key message

A novel interspecific somatic hybrid combining drought tolerance and high quality of sweet potato and Ipomoea triloba L. was obtained and its genetic and epigenetic variations were studied.

Abstract

Somatic hybridization can be used to overcome the cross-incompatibility between sweet potato (Ipomoea batatas (L.) Lam.) and its wild relatives and transfer useful and desirable genes from wild relatives to cultivated plants. However, most of the interspecific somatic hybrids obtained to date cannot produce storage roots and do not exhibit agronomic characters. In the present study, a novel interspecific somatic hybrid, named XT1, was obtained through protoplast fusion between sweet potato cv. Xushu 18 and its wild relative I. triloba. This somatic hybrid produced storage roots and exhibited significantly higher drought tolerance and quality compared with its cultivated parent Xushu 18. Transcriptome and real-time quantitative PCR (qRT-PCR) analyses revealed that the well-known drought stress-responsive genes in XT1 and I. triloba were significantly up-regulated under drought stress. The genomic structural reconstructions between the two genomes of the fusion parents in XT1 were confirmed using genomic in situ hybridization (GISH) and specific nuclear and cytoplasmic DNA markers. The DNA methylation variations were characterized by methylation-sensitive amplified polymorphism (MSAP). This study not only reveals the significance of somatic hybridization in the genetic improvement of sweet potato but also provides valuable materials and knowledge for further investigating the mechanism of storage root formation in sweet potato.

Highlights

  • A novel interspecific somatic hybrid XT1 with high drought tolerance and quality was obtained between sweetpotato and I. triloba.

  • Genetic and epigenetic variations of XT1 were clarified.

  • This study provides valuable materials and knowledge for investigating the mechanism of storage root formation in sweetpotato.

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Funding

This work was supported by the National Key R&D Program of China (2019YFD1001302/2019YFD1001300) and the earmarked fund for CARS-10-Sweet potato.

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  1. Licong Jia and Yufeng Yang contributed equally to this work.

Authors and Affiliations

  1. Key Laboratory of Sweet Potato Biology and Biotechnology, Ministry of Agriculture and Rural Affairs/Beijing Key Laboratory of Crop Genetic Improvement/Laboratory of Crop Heterosis & Utilization and Joint Laboratory for International Cooperation in Crop Molecular Breeding, Ministry of Education, College of Agronomy & Biotechnology, China Agricultural University, Beijing, 100193, China

    Licong Jia, Yufeng Yang, Hong Zhai, Shaozhen He, Ning Zhao, Huan Zhang, Shaopei Gao & Qingchang Liu

  2. Institute of Grain and Oil Crops, Yantai Academy of Agricultural Sciences, Yantai, 265500, China

    Licong Jia & Guosheng Xin

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  1. Licong Jia
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  3. Hong Zhai
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  4. Shaozhen He
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Contributions

QL, LJ and HZhai conceived and designed the experiments. LJ and YY performed the experiments. LJ, YY, SH, GX, NZ, HZhang and SG analyzed the data. QL, LJ and YY wrote the manuscript. All authors read and approved the final manuscript.

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Correspondence to Qingchang Liu.

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Communicated by Teodoro Cardi.

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Jia, L., Yang, Y., Zhai, H. et al. Production and characterization of a novel interspecific somatic hybrid combining drought tolerance and high quality of sweet potato and Ipomoea triloba L.. Plant Cell Rep 41, 2159–2171 (2022). https://doi.org/10.1007/s00299-022-02912-8

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