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Nuclear and cytoplasmic genome components of Solanum tuberosum + S. chacoense somatic hybrids and three SSR alleles related to bacterial wilt resistance

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Abstract

The somatic hybrids were derived previously from protoplast fusion between Solanum tuberosum and S. chacoense to gain the bacterial wilt resistance from the wild species. The genome components analysis in the present research was to clarify the nuclear and cytoplasmic composition of the hybrids, to explore the molecular markers associated with the resistance, and provide information for better use of these hybrids in potato breeding. One hundred and eight nuclear SSR markers and five cytoplasmic specific primers polymorphic between the fusion parents were used to detect the genome components of 44 somatic hybrids. The bacterial wilt resistance was assessed thrice by inoculating the in vitro plants with a bacterial suspension of race 1. The disease index, relative disease index, and resistance level were assigned to each hybrid, which were further analyzed in relation to the molecular markers for elucidating the potential genetic base of the resistance. All of the 317 parental unique nuclear SSR alleles appeared in the somatic hybrids with some variations in the number of bands detected. Nearly 80 % of the hybrids randomly showed the chloroplast pattern of one parent, and most of the hybrids exhibited a fused mitochondrial DNA pattern. One hundred and nine specific SSR alleles of S. chacoense were analyzed for their relationship with the disease index of the hybrids, and three alleles were identified to be significantly associated with the resistance. Selection for the resistant SSR alleles of S. chacoense may increase the possibility of producing resistant pedigrees.

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Acknowledgments

The authors thank Professor Jeff Moorby for critical reading and language editing of this manuscript. The work was supported by the Natural Science Foundation of Hubei Province (2009CDA085).

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Authors and Affiliations

  1. National Center for Vegetable Improvement (Central China), Key Laboratory of Horticultural Plant Biology, Ministry of Education, Potato Engineering and Technology Research Center of Hubei Province, Huazhong Agricultural University, Wuhan, 430070, Hubei, People’s Republic of China

    Lin Chen, Xianpu Guo, Conghua Xie, Li He, Xingkui Cai, Lingli Tian, Botao Song & Jun Liu

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  1. Lin Chen
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  2. Xianpu Guo
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  3. Conghua Xie
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  4. Li He
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  8. Jun Liu
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Correspondence to Jun Liu.

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Communicated by D. Lightfoot.

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Chen, L., Guo, X., Xie, C. et al. Nuclear and cytoplasmic genome components of Solanum tuberosum + S. chacoense somatic hybrids and three SSR alleles related to bacterial wilt resistance. Theor Appl Genet 126, 1861–1872 (2013). https://doi.org/10.1007/s00122-013-2098-5

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  • DOI: https://doi.org/10.1007/s00122-013-2098-5

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