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Development and evaluation of a storage root-bearing sweetpotato somatic hybrid between Ipomoea batatas (L.) Lam. and I. triloba L.

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Abstract

A storage root-bearing somatic hybrid was produced for the first time by protoplast fusion between sweetpotato (Ipomoea batatas (L.) Lam.) cv. Kokei No. 14 and its wild relative I. triloba L. Protoplasts isolated from embryogenic suspension cultures of Kokei No. 14 were fused with petiole protoplasts of I. triloba L. using polyethylene glycol-mediated protocol. Fusion products were cultured in a modified Murashige and Skoog medium containing 0.05 mg l−1 2,4-dichlorophenoxyacetic acid (2,4-D) and 0.5 mg l−1 kinetin. A total of 176 plants were obtained from 42 out of 134 calluses derived from fused protoplasts, and 91 of these plants were confirmed to be somatic hybrids through peroxidase isozyme, random amplified polymorphic DNA, amplified fragment length polymorphism, and cytological analyses. Upon transfer into soil and grown in the greenhouse and then to the field, 100% survival was observed. A single plant, designated KT1, was found to produce storage roots. Genomic in situ hybridization analysis confirmed presence of chromosomes from both parents and recombinant chromosomes in KT1. Drought tolerance, dry matter content, soluble sugar content, and fertility of this somatic hybrid were evaluated for potential use in sweetpotato breeding.

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Abbreviations

BA:

Benzyladenine

CAT:

Catalase

DAPI:

4,6-Diamidino-2-phenylindole

2,4-D:

2,4-Dichlorophenoxyacetic acid

GISH:

Genomic in situ hybridization

MS:

Murashige and Skoog (1962) medium

PAGE:

Polyacrylamide gel electrophoresis

PEG:

Polyethylene glycol

PVP:

Polyvinylpyrrolidone

RWC:

Relative water content

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Acknowledgments

This work was supported by the National “863 Project” of China (no. 2006AA100107), the National Science and Technology Project of China (no. 2006BAD01A06, 2006BAD13B06), the National Project for Public Industry of China (no. nyhyzx07-012) and “948 Project” for China Agriculture (no. 2006G21). The authors thank Prof. Weiwei Jin of China Agricultural University and his research group members for their help in performing the GISH analysis.

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Authors and Affiliations

  1. Key Laboratory of Crop Genomics and Genetic Improvement, Ministry of Agriculture, China Agricultural University, 100193, Beijing, China

    Y. Yang, S. Guan, H. Zhai, S. He & Q. Liu

  2. Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, 100193, Beijing, China

    H. Zhai, S. He & Q. Liu

  3. Laboratory of Crop Heterosis and Utilization, Ministry of Education, China Agricultural University, 100193, Beijing, China

    Q. Liu

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  1. Y. Yang
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  2. S. Guan
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  3. H. Zhai
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  4. S. He
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  5. Q. Liu
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Correspondence to Q. Liu.

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Yang, Y., Guan, S., Zhai, H. et al. Development and evaluation of a storage root-bearing sweetpotato somatic hybrid between Ipomoea batatas (L.) Lam. and I. triloba L.. Plant Cell Tiss Organ Cult 99, 83–89 (2009). https://doi.org/10.1007/s11240-009-9578-y

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  • DOI: https://doi.org/10.1007/s11240-009-9578-y

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