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Identification of different cytoplasms based on newly developed mitotype-specific markers for marker-assisted selection breeding in Brassica napus L.

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Different mitotype-specific markers were developed to distinguish different cytoplasms in Brassica napus L.

Abstract

Mitotype-specific markers have been developed to distinguish different mitotypes in plant. And use of molecular markers to identify different mitotypes in Brassica napus would enhance breeding efficiency. Here, we comparatively analyzed six sequenced mitochondrial genomes in Brassica napus and identified collinear block sequences and mitotype-specific sequences (MSSs) of these mitochondrial genomes. The collinear block sequences between mitochondrial genomes of nap, cam, and pol cytoplasmic male sterility (CMS) lines were higher than those of other lines. After comparative analysis of the six sequenced mitochondrial genomes (cam, nap, ole, pol CMS, ogu CMS, and hau CMS), 90 MSSs with sizes ranging from 101 to 9981 bp and a total length of 103,756 bp (accounting for 6.77% of the mitochondrial genome sequences) were identified. Additionally, 12 mitotype-specific markers were developed based on the mitochondrial genome-specific sequences in order to distinguish among these different mitotypes. Cytoplasms of 570 different inbred lines collected across scientific research institutes in China were identified using the MSS markers developed in our study. In addition to confirming the accuracy of the cytoplasmic identification, we also identified mitotypes that have not been reported in Brassica napus. Our study may provide guidance for the classification of different mitotypes in B. napus breeding.

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Acknowledgements

We sincerely thank Professor Rongzhan Guan for his kindly provide the seeds and mtDNAs of Suzhongqing (B. rapa) and B. oleracea. This research was financially supported by the National Natural Science Foundation of China (NSFC Grant Number 31271761), the National Key Research and Development Program of China (Grant Number 2016YFD0101300), the Program for Modern Agricultural Industrial Technology System (nycytx-00501) and Nanhu Scholars Program for Young Scholars of XYNU.

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Authors and Affiliations

  1. National Key Laboratory of Crop Genetic Improvement, National Engineering Research Center of Rapeseed, Huazhong Agricultural University, Wuhan, 430070, People’s Republic of China

    Shuangping Heng, Fengyi Chen, Chao Wei, Kaining Hu, Zonghui Yang, Jing Wen, Bin Yi, Chaozhi Ma, Jinxing Tu, Tingdong Fu & Jinxiong Shen

  2. College of Life Science, Xinyang Normal University, Xinyang, 464000, People’s Republic of China

    Shuangping Heng

  3. Centre for Plant Genetics and Breeding, School of Plant Biology, The University of Western Australia, 35 Stirling Highway, Crawley, WA, 6009, Australia

    Ping Si

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  1. Shuangping Heng
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  2. Fengyi Chen
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  3. Chao Wei
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  4. Kaining Hu
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  5. Zonghui Yang
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Correspondence to Jinxiong Shen.

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Additional information

Communicated by Kinya Toriyama.

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299_2017_2121_MOESM1_ESM.xls

Table S1 The distribution of different MSS (> 100 bp) in different mitochondrial genomes used in this study. MSS1 to MSS90 were the sequences corresponding to the distribution of MSSs in Fig. 2. The detailed places of these MSSs from different mitochondrial genomes were showed in it. The number “0” represent no homology sequences existed. (XLS 24 KB)

Table S2 Detailed mitotype information of 570 B. napus inbred lines used in this study. (XLS 77 KB)

Table S3 The PCR primers used in this study. (XLS 334 KB)

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Heng, S., Chen, F., Wei, C. et al. Identification of different cytoplasms based on newly developed mitotype-specific markers for marker-assisted selection breeding in Brassica napus L.. Plant Cell Rep 36, 901–909 (2017). https://doi.org/10.1007/s00299-017-2121-4

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