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Genetic characterization of a new cytoplasmic male sterility system (hau) in Brassica juncea and its transfer to B. napus

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Abstract

A novel cytoplasmic male sterility (CMS) was identified in Brassica juncea, named as hau CMS (00-6-102A). Subsequently, the male sterility was transferred to B. napus by interspecific hybridization. The hau CMS has stable male sterility. Flowers on the A line are absolutely male sterile, and seeds harvested from the line following pollinations with the maintainer gave rise to 100% sterile progeny. The anthers in CMS plants are replaced by thickened petal-like structures and pollen grains were not detected. In contrast, in other CMS systems viz. pol, nap, tour, and ogu, anthers are formed but do not produce viable pollen. The sterility of hau CMS initiates at the stage of stamen primordium polarization, which is much earlier compared with the other four CMS systems. We have successfully transferred hau CMS from B. juncea to B. napus. Restorer lines for pol, ogu, nap, and tour CMS systems were found to be ineffective to restore fertility in hau CMS. Sixteen out of 40 combinations of mitochondrial probe/enzyme used for RFLP analysis distinguished the hau CMS system from the other four systems. Among these sixteen combinations, five ones alone could distinguish the five CMS systems from each other. The evidence from genetic, morphological, cytological and molecular studies confirmed that the hau CMS system is a novel CMS system.

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Acknowledgments

This research was supported by The National Programs on the Development of Basic Research Special Foundation of China (2001CB1088), special “948” program (2003-Q04) of China and The Program for Changjiang Scholars and Innovative Research Team for Universities (IRT0442). The authors are grateful to Professor Shyam Prakash from Indian Agricultural Research Institute, Professor Qifa Zhang in Huazhong Agricultural University, Dr. Wallace Cowling, Dr. Sheng Chen and Dr. Guijun Yan in the University of Western Australian for critical reading of the manuscript.

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  1. National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, 430070, China

    Zhengjie Wan, Bing Jing, Jinxing Tu, Caozhi Ma, Jinxiong Shen, Bin Yi, Jing Wen, Tao Huang, Xianjun Wang & Tingdong Fu

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  1. Zhengjie Wan
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  2. Bing Jing
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  3. Jinxing Tu
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Correspondence to Tingdong Fu.

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Communicated by C. F. Quiros.

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122_2007_673_MOESM1_ESM.doc

Table S1 RFLP patterns of CMS and maintainer lines. Numbers in bold indicate the banding patterns from each probe and restriction enzyme combination. Size of bands is indicated in kb (DOC 58 KB).

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Wan, Z., Jing, B., Tu, J. et al. Genetic characterization of a new cytoplasmic male sterility system (hau) in Brassica juncea and its transfer to B. napus . Theor Appl Genet 116, 355–362 (2008). https://doi.org/10.1007/s00122-007-0673-3

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