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Construction of a high-density linkage map and mapping quantitative trait loci for somatic embryogenesis using leaf petioles as explants in upland cotton (Gossypium hirsutum L.)

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Abstract

Key message

The first high-density linkage map was constructed to identify quantitative trait loci (QTLs) for somatic embryogenesis (SE) in cotton ( Gossypium hirsutum L.) using leaf petioles as explants.

Abstract

Cotton transformation is highly limited by only a few regenerable genotypes and the lack of understanding of the genetic and molecular basis of somatic embryogenesis (SE) in cotton (Gossypium hirsutum L.). To construct a more saturated linkage map and further identify quantitative trait loci (QTLs) for SE using leaf petioles as explants, a high embryogenesis frequency line (W10) from the commercial Chinese cotton cultivar CRI24 was crossed with TM-1, a genetic standard upland cotton with no embryogenesis frequency. The genetic map spanned 2300.41 cM in genetic distance and contained 411 polymorphic simple sequence repeat (SSR) loci. Of the 411 mapped loci, 25 were developed from unigenes identified for SE in our previous study. Six QTLs for SE were detected by composite interval mapping method, each explaining 6.88–37.07 % of the phenotypic variance. Single marker analysis was also performed to verify the reliability of QTLs detection, and the SSR markers NAU3325 and DPL0209 were detected by the two methods. Further studies on the relatively stable and anchoring QTLs/markers for SE in an advanced population of W10 × TM-1 and other cross combinations with different SE abilities may shed light on the genetic and molecular mechanism of SE in cotton.

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Acknowledgments

We wish to thank Dr.Chee-kok Chin from Rutgers University, Jinfa Zhang from New Mexico State University, and Jiahe Wu from the Chinese Academy of Sciences for writing assistance and critical suggestions. This work was supported by the National Science Fund for Distinguished Young Scholars (31125020) and the Innovation Scientists and Technicians Troop Construction Projects of Henan Province.

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The authors declare that they have no conflict of interest.

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    Authors and Affiliations

    1. State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, 455000, China

      Zhenzhen Xu, Chaojun Zhang, Xiaoyang Ge, Ni Wang, Kehai Zhou, Xiaojie Yang, Zhixia Wu, Xueyan Zhang, Chuanliang Liu, Zuoren Yang, Changfeng Li, Kun Liu, Zhaoen Yang, Yuyuan Qian & Fuguang Li

    2. Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China

      Zhenzhen Xu

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    Correspondence to Fuguang Li.

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    Communicated by X. S. Zhang.

    Z. Xu, C. Zhang, and X. Ge contributed equally to this work.

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    Xu, Z., Zhang, C., Ge, X. et al. Construction of a high-density linkage map and mapping quantitative trait loci for somatic embryogenesis using leaf petioles as explants in upland cotton (Gossypium hirsutum L.). Plant Cell Rep 34, 1177–1187 (2015). https://doi.org/10.1007/s00299-015-1776-y

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