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Inclusive composite interval mapping (ICIM) for digenic epistasis of quantitative traits in biparental populations

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Abstract

It has long been recognized that epistasis or interactions between non-allelic genes plays an important role in the genetic control and evolution of quantitative traits. However, the detection of epistasis and estimation of epistatic effects are difficult due to the complexity of epistatic patterns, insufficient sample size of mapping populations and lack of efficient statistical methods. Under the assumption of additivity of QTL effects on the phenotype of a trait in interest, the additive effect of a QTL can be completely absorbed by the flanking marker variables, and the epistatic effect between two QTL can be completely absorbed by the four marker-pair multiplication variables between the two pairs of flanking markers. Based on this property, we proposed an inclusive composite interval mapping (ICIM) by simultaneously considering marker variables and marker-pair multiplications in a linear model. Stepwise regression was applied to identify the most significant markers and marker-pair multiplications. Then a two-dimensional scanning (or interval mapping) was conducted to identify QTL with significant digenic epistasis using adjusted phenotypic values based on the best multiple regression model. The adjusted values retain the information of QTL on the two current mapping intervals but exclude the influence of QTL on other intervals and chromosomes. Epistatic QTL can be identified by ICIM, no matter whether the two interacting QTL have any additive effects. Simulated populations and one barley doubled haploids (DH) population were used to demonstrate the efficiency of ICIM in mapping both additive QTL and digenic interactions.

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Acknowledgments

This work was supported by the National 973 and 863 Programs of China (2006CB101700 and 2006AA10Z1B1), and the Generation Challenge Program of the Consultative Group for International Agricultural Research.

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Authors and Affiliations

  1. School of Mathematical Sciences, Beijing Normal University, Beijing, 100875, China

    Huihui Li & Zhonglai Li

  2. Generation Challenge Programme, c/o CIMMYT, Apdo. Postal 6-641, Mexico, D.F., 06600, Mexico

    Jean-Marcel Ribaut

  3. The National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science and CIMMYT China Office, Chinese Academy of Agricultural Sciences, Beijing, 100081, China

    Huihui Li & Jiankang Wang

Authors
  1. Huihui Li
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  2. Jean-Marcel Ribaut
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  3. Zhonglai Li
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  4. Jiankang Wang
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Corresponding author

Correspondence to Jiankang Wang.

Additional information

Communicated by M. Sillanpää.

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Li, H., Ribaut, JM., Li, Z. et al. Inclusive composite interval mapping (ICIM) for digenic epistasis of quantitative traits in biparental populations. Theor Appl Genet 116, 243–260 (2008). https://doi.org/10.1007/s00122-007-0663-5

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  • DOI: https://doi.org/10.1007/s00122-007-0663-5

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