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Metabolic engineering strategies for enhanced shikimate biosynthesis: current scenario and future developments

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Abstract

Shikimic acid is an important intermediate for the manufacture of the antiviral drug oseltamivir (Tamiflu®) and many other pharmaceutical compounds. Much of its existing supply is obtained from the seeds of Chinese star anise (Illicium verum). Nevertheless, plants cannot supply a stable source of affordable shikimate along with laborious and cost-expensive extraction and purification process. Microbial biosynthesis of shikimate through metabolic engineering and synthetic biology approaches represents a sustainable, cost-efficient, and environmentally friendly route than plant-based methods. Metabolic engineering allows elevated shikimate production titer by inactivating the competing pathways, increasing intracellular level of key precursors, and overexpressing rate-limiting enzymes. The development of synthetic and systems biology-based novel technologies have revealed a new roadmap for the construction of high shikimate-producing strains. This review elaborates the enhanced biosynthesis of shikimate by utilizing an array of traditional metabolic engineering along with novel advanced technologies. The first part of the review is focused on the mechanistic pathway for shikimate production, use of recombinant and engineered strains, improving metabolic flux through the shikimate pathway, chemically inducible chromosomal evolution, and bioprocess engineering strategies. The second part discusses a variety of industrially pertinent compounds derived from shikimate with special reference to aromatic amino acids and phenazine compound, and main engineering strategies for their production in diverse bacterial strains. Towards the end, the work is wrapped up with concluding remarks and future considerations.

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Funding

This work was supported by the National Natural Science Foundation of China (No. 31670033).

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Authors and Affiliations

  1. State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, China

    Muhammad Bilal, Songwei Wang, Hongbo Hu, Wei Wang & Xuehong Zhang

  2. School of Life Science and Food Engineering, Huaiyin Institute of Technology, Huaian, 223003, China

    Muhammad Bilal & Yuping Zhao

  3. Tecnologico de Monterrey, School of Engineering and Sciences, Campus Monterrey, Ave. Eugenio Garza Sada 2501, CP 64849, Monterrey, NL, Mexico

    Hafiz M. N. Iqbal

  4. National Experimental Teaching Center for Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, China

    Hongbo Hu

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  1. Muhammad Bilal
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  2. Songwei Wang
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  3. Hafiz M. N. Iqbal
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  4. Yuping Zhao
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  5. Hongbo Hu
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  6. Wei Wang
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  7. Xuehong Zhang
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Correspondence to Hongbo Hu.

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Bilal, M., Wang, S., Iqbal, H.M.N. et al. Metabolic engineering strategies for enhanced shikimate biosynthesis: current scenario and future developments. Appl Microbiol Biotechnol 102, 7759–7773 (2018). https://doi.org/10.1007/s00253-018-9222-z

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  • DOI: https://doi.org/10.1007/s00253-018-9222-z

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