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Metabolic engineering of Escherichia coli for production of chemicals derived from the shikimate pathway

  • Metabolic Engineering and Synthetic Biology - Mini Review
  • Published:
Journal of Industrial Microbiology & Biotechnology

Abstract

The shikimate pathway is indispensable for the biosynthesis of natural products with aromatic moieties. These products have wide current and potential applications in food, cosmetics and medicine, and consequently have great commercial value. However, compounds extracted from various plants or synthesized from petrochemicals no longer satisfy the requirements of contemporary industries. As a result, an increasing number of studies has focused on this pathway to enable the biotechnological manufacture of natural products, especially in E. coli. Furthermore, the development of synthetic biology, systems metabolic engineering and high flux screening techniques has also contributed to improving the biosynthesis of high-value compounds based on the shikimate pathway. Here, we review approaches based on a combination of traditional and new metabolic engineering strategies to increase the metabolic flux of the shikimate pathway. In addition, applications of this optimized pathway to produce aromatic amino acids and a range of natural products is also elaborated. Finally, this review sums up the opportunities and challenges facing this field.

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Acknowledgements

This work was supported by the National Key R&D Program of China (2018YFA0900302), the National Natural Science Foundation of China (21621004, 31900052), the Tianjin Science Fund for Distinguished Young Scholars (17JCJQJC45300), the Science and Technology Service Network (STS) Initiative of the Chinese Academy of Sciences (KFJ-STS-ZDTP-065), and he National Key R&D Program of China (2018YFA0901600).

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Authors and Affiliations

  1. Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (Ministry of Education); SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, China

    Zhu Li, Zhishuai Chang & Tao Chen

  2. Key Laboratory of Systems Microbial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, China

    Zhu Li, Huiying Wang, Dongqin Ding, Yongfei Liu, Huan Fang & Dawei Zhang

  3. Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, China

    Zhu Li, Huiying Wang, Dongqin Ding, Yongfei Liu, Huan Fang & Dawei Zhang

  4. University of Chinese Academy of Sciences, Beijing, 100049, China

    Dongqin Ding & Dawei Zhang

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  1. Zhu Li
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Li, Z., Wang, H., Ding, D. et al. Metabolic engineering of Escherichia coli for production of chemicals derived from the shikimate pathway. J Ind Microbiol Biotechnol 47, 525–535 (2020). https://doi.org/10.1007/s10295-020-02288-2

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