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Applied Microbiology and Biotechnology

, Volume 99, Issue 21, pp 8943–8954 | Cite as

Screening, identification, and characterization of a GH43 family β-xylosidase/α-arabinofuranosidase from a compost microbial metagenome

  • Tomohiko Matsuzawa
  • Satoshi Kaneko
  • Katsuro YaoiEmail author
Biotechnologically relevant enzymes and proteins

Abstract

A putative glycoside hydrolase family 43 β-xylosidase/α-arabinofuranosidase (CoXyl43) that promotes plant biomass saccharification was isolated via functional screening of a compost microbial metagenomic library and characterized. CoXyl43 promoted the saccharification of plant biomasses, including xylans (xylan and arabinoxylan), rice straw, and Erianthus, by degrading xylooligosaccharide residues to monosaccharide residues. The recombinant CoXyl43 protein exhibited both β-xylosidase and α-arabinofuranosidase activities for chromogenic substrates, with optimal activity at pH 7.5 and 55 °C. Both of these activities were inactivated by ethanol, dimethylsulfoxide, and zinc and copper ions but were activated by manganese ions. Only the β-xylosidase activity of recombinant CoXyl43 was enhanced in the presence of calcium ions. These results indicate that CoXyl43 exhibits unique enzymatic properties useful for biomass saccharification.

Keywords

β-xylosidase α-arabinofuranosidase Biomass Metagenome Glycoside hydrolase family 43 

Notes

Acknowledgments

We thank Dr. Y. Kobayashi (Japan Bioindustry Association) for provision of the biomass materials and helpful discussion. This work was supported in part by grants from the New Energy and Industrial Technology Development Organization (NEDO).

Conflict of interest

The authors declare no conflicts of interest.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • Tomohiko Matsuzawa
    • 1
  • Satoshi Kaneko
    • 2
    • 3
  • Katsuro Yaoi
    • 1
    Email author
  1. 1.Bioproduction Research InstituteNational Institute of Advanced Industrial Science and Technology (AIST)TsukubaJapan
  2. 2.Food Biotechnology DivisionNational Food Research InstituteTsukubaJapan
  3. 3.Department of Subtropical Biochemistry and Biotechnology, Faculty of AgricultureUniversity of the RyukyusNishiharaJapan

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