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Isolation and Characterization of a GDSL Esterase from the Metagenome of a Marine Sponge-associated Bacteria

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Abstract

Using a metagenome library constructed from a bacterial associated with a marine sponge Hyrtios erecta, we identified a novel esterase that belongs to the SGNH hydrolase superfamily of esterases. The substrate specificity of EstHE1 was determined using p-nitrophenyl (pNP) ester (C2: acetate, C4: butylate, C6: caproate, C12: laurate, C16: palmitate). EstHE1 exhibited activity against C2 (5.6 U/mg), C4 (5.1 U/mg), and C6 (2.8 U/mg) substrates. The optimal temperature for EstHE1 esterase activity of the pNP acetate substrate was 40°C, and EstHE1 retained 60% of its enzymatic activity in the 30–50°C range. This esterase showed moderate thermostability, retaining 58% of its activity even after preincubation for 12 h at 40°C. EstHE1 also maintained activity in high concentrations of NaCl, indicating that this esterase is salt-tolerant. Thus, EstHE1 has the thermal stability and salt tolerance necessary for use as an industrial enzyme.

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Acknowledgment

This work was funded in part by the New Energy and Industrial Technology Development Organization (NEDO) as part of a research and development project of the Industrial Science and Technology Frontier Program.

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Authors and Affiliations

  1. Department of Life Science and Medical Bio-Science, Waseda University, 2-2 Wakamatsu-cho, Shinjuku, Tokyo, 162-8480, Japan

    Yoshiko Okamura & Haruko Takeyama

  2. Consolidated Research Institute for Advanced Science and Medical Care, 513 Waseda-Tsurumaki-chi, Shinjuku, Tokyo, 162-0041, Japan

    Yoshiko Okamura, Hiroko Yokouchi, Tadashi Matsunaga & Haruko Takeyama

  3. Department of Biotechnology, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei, Tokyo, 184-8588, Japan

    Yoshiko Okamura, Tomonori Kimura, Macarena Meneses-Osorio, Tadashi Matsunaga & Haruko Takeyama

  4. Seikai National Fisheries Research Institute, Fisheries Research Agency, Okinawa, Japan

    Masaya Katoh

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  1. Yoshiko Okamura
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  2. Tomonori Kimura
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  3. Hiroko Yokouchi
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  4. Macarena Meneses-Osorio
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  5. Masaya Katoh
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  6. Tadashi Matsunaga
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  7. Haruko Takeyama
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Correspondence to Haruko Takeyama.

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Okamura, Y., Kimura, T., Yokouchi, H. et al. Isolation and Characterization of a GDSL Esterase from the Metagenome of a Marine Sponge-associated Bacteria. Mar Biotechnol 12, 395–402 (2010). https://doi.org/10.1007/s10126-009-9226-x

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  • DOI: https://doi.org/10.1007/s10126-009-9226-x

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