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Co-production of 3-hydroxypropionic acid and 1,3-propanediol from glycerol using resting cells of recombinant Klebsiella pneumoniae J2B strain overexpressing aldehyde dehydrogenase

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Abstract

The co-production of 3-hydroxypropionic acid (3HP) and 1,3-propanediol (PDO) from glycerol was studied using the resting cells of a recombinant Klebsiella pneumoniae J2B strain that overexpresses an aldehyde dehydrogenase (KGSADH). Active biomass was produced in a mineral salt medium containing yeast extract and glycerol under a range of aeration conditions, and shifted to potassium phosphate buffer containing glycerol for bioconversion. The microaerobic or anaerobic conditions were favorable for both the production of active biomass and subsequent bioconversion. At the flask level, the recombinant strain (2.0 g CDW/L) grown under microaerobic conditions produced 43.2 mM 3HP and 59.0 mM PDO from glycerol (117 mM) in 30 min with a cumulative yield of 0.87 (mol/mol). The fed-batch bioconversion, which was performed in a 1.5-L bioreactor with 1.0 g CDW/L at a constant pH 7.0 under anaerobic conditions, resulted in 125.6 mM 3HP and 209.5 mM PDO in 12 h with a cumulative overall productivity, yield, and maximum specific production rate of 27.9 mmol/L/h, 0.71 (mol/mol), and 128.5 mmol/g CDW/h, respectively. Lactate, succinate and 2,3-butanediol were the major by-products, whereas the production of acetate and ethanol was marginal. This is the first report of the simultaneous production of 3HP and PDO from glycerol using a resting cell system.

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Acknowledgments

This study was supported financially by Korean Ministry of Education, Science and Technology through Advanced Biomass R&D Center (ABC; Grant No. 2010-0029799), KAIST, Korea. We are grateful to the Brain Korea 21 program and the 2012 Post-Doc Development Program (Dr. Vinod Kumar), Pusan National University for their financial assistance.

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Authors and Affiliations

  1. Department of Chemical and Biomolecular Engineering, Pusan National University, San 30, Jangjeon-dong, Geumjeong-gu, Busan, 609-735, South Korea

    Vinod Kumar, Mugesh Sankaranarayanan, Kyeung-eun Jae, Meetu Durgapal, Somasundar Ashok, Yeounjoo Ko, Ritam Sarkar & Sunghoon Park

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  1. Vinod Kumar
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  2. Mugesh Sankaranarayanan
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  3. Kyeung-eun Jae
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  4. Meetu Durgapal
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  5. Somasundar Ashok
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  6. Yeounjoo Ko
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  7. Ritam Sarkar
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  8. Sunghoon Park
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Correspondence to Sunghoon Park.

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Kumar, V., Sankaranarayanan, M., Jae, Ke. et al. Co-production of 3-hydroxypropionic acid and 1,3-propanediol from glycerol using resting cells of recombinant Klebsiella pneumoniae J2B strain overexpressing aldehyde dehydrogenase. Appl Microbiol Biotechnol 96, 373–383 (2012). https://doi.org/10.1007/s00253-012-4187-9

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  • DOI: https://doi.org/10.1007/s00253-012-4187-9

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