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Identification and utilization of a 1,3-propanediol oxidoreductase isoenzyme for production of 1,3-propanediol from glycerol in Klebsiella pneumoniae

  • Applied Genetics and Molecular Biotechnology
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Abstract

In a previous study, we showed that 1,3-propanediol (1,3-PD) was still produced from glycerol by the Klebsiella pneumoniae mutant strain defective in 1,3-PD oxidoreductase (DhaT), although the production level was lower compared to the parent strain. As a potential candidate for another putative 1,3-PD oxidoreductase, we identified and characterized a homolog of Escherichia coli yqhD (88% homology in amino acid sequence), which encodes an alcohol dehydrogenase and is well known to replace the function of DhaT in E. coli. Introduction of multiple copies of the yqhD homolog restored 1,3-PD production in the mutant K. pneumoniae strain defective in DhaT. In addition, by-product formation was still eliminated in the recombinant strain due to the elimination of the glycerol oxidative pathway. An increase in NADP-dependent 1,3-PD oxidoreductase activity was observed in the recombinant strain harboring multiple copies of the yqhD homolog. The level of 1,3-PD production during batch fermentation in the recombinant strain was comparable to that of the parent strain; further engineering can generate an industrial strain producing 1,3-propanediol.

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Acknowledgments

This study was supported by a grant from the KRIBB Research Initiative Program.

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Authors and Affiliations

  1. Molecular Bioprocess Research Center, Jeonbuk Branch Institute, KRIBB, Jeongeup, Jeonbuk, 580-185, South Korea

    Jeong-Woo Seo, Mi-Young Seo, Baek-Rock Oh, Sun-Yeon Heo, Jin-Oh Baek, Dina Rairakhwada, Lian Hua Luo, Won-Kyung Hong & Chul Ho Kim

  2. School of Life Sciences and Biotechnology, Korea University, Seoul, 136-701, South Korea

    Jin-Oh Baek

  3. School of Biological Sciences and Technology, Chonnam National University, Gwangju, 500-757, South Korea

    Baek-Rock Oh

  4. Institute for Molecular Biology and Genetics, Research Center of Bioactive Materials, Chonbuk National University, Jeonju, Chonbuk, 561-756, South Korea

    Lian Hua Luo

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  1. Jeong-Woo Seo
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  2. Mi-Young Seo
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  8. Won-Kyung Hong
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  9. Chul Ho Kim
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Correspondence to Chul Ho Kim.

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Seo, JW., Seo, MY., Oh, BR. et al. Identification and utilization of a 1,3-propanediol oxidoreductase isoenzyme for production of 1,3-propanediol from glycerol in Klebsiella pneumoniae . Appl Microbiol Biotechnol 85, 659–666 (2010). https://doi.org/10.1007/s00253-009-2123-4

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  • DOI: https://doi.org/10.1007/s00253-009-2123-4

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