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Integrated Assessment of Biomarker Response in Carp (Cyprinus carpio) and Silver Catfish (Rhamdia quelen) Exposed to Clomazone

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Abstract

Clomazone is considered a potential contaminant of groundwater and is persistent in the environment. To verify the effects of clomazone in Cyprinus carpio and Rhamdia quelen, a method that combines biomarker responses into an index of “integrated biomarker response” (IBR) was used for observed biological alterations in these species. Thiobarbituric acid-reactive substances in liver of carp and silver catfish decreased at both concentrations tested. However, in muscle it increased in carp at 3 mg/L and silver catfish at 6 mg/L. Protein carbonyl increased in liver (3 and 6 mg/L) and muscle (6 mg/L) of carp. In carp, superoxide dismutase (SOD) increased at 3 mg/L and catalase at 6 mg/L. In silver catfish, SOD in liver decreased at 3 mg/L. Glutathione-S-transferase increased at 3 mg/L in muscle of carp. Nonprotein thiol levels decreased at both concentrations in liver of silver catfish and muscle of carp. In silver catfish, acetylcholinesterase (AChE) decreased in brain at 6 mg/L. Nevertheless, AChE in muscle of both species increased at 3 and 6 mg/L. IBR was standardized scores of biomarker responses and was visualized using star plots. The IBR values shown that in carp there was predominantly an induction of parameters, whereas in silver catfish there was inhibition of these responses. In this way, IBR may be a practical tool for the identification of biological alterations in fish exposed to pesticides. In the present study, IBR was efficient for comparisons of fish species using clomazone. This study may serve as a base for evaluation of other pesticides in the rice field, environment, or laboratory experiment.

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Acknowledgments

We thank the Federal University of Santa Maria for the support and facilities and the financial support and fellowships from the Brazilian agency Coordination for the Improvement of Higher Education Personnel.

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Authors and Affiliations

  1. Programa de Pós-graduação em Bioquímica Toxicológica, Departamento de Bioquímica e Biologia Molecular, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Santa Maria, RS, 97105-900, Brazil

    Camila R. Murussi, Maiara Costa, Charlene Menezes, Jossiele Leitemperger, Luciana Guerra, Thais Lópes, Eduardo Severo & Vania Lucia Loro

  2. Laboratório de Análises de Resíduos de Pesticidas, Departamento de Química, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Santa Maria, RS, 97105-900, Brazil

    Renato Zanella

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  1. Camila R. Murussi
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  4. Jossiele Leitemperger
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Correspondence to Vania Lucia Loro.

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Murussi, C.R., Costa, M., Menezes, C. et al. Integrated Assessment of Biomarker Response in Carp (Cyprinus carpio) and Silver Catfish (Rhamdia quelen) Exposed to Clomazone. Arch Environ Contam Toxicol 68, 646–654 (2015). https://doi.org/10.1007/s00244-015-0145-8

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