Skip to main content
SpringerLink
Log in

Tissue Biochemical Alterations of Cyprinus carpio Exposed to Commercial Herbicide Containing Clomazone Under Rice-Field Conditions

  • Published:
Archives of Environmental Contamination and Toxicology Aims and scope Submit manuscript

Abstract

Field and laboratory experiments were performed to evaluate toxicological responses of Cyprinus carpio exposed to the commercial herbicide clomazone (500 mg l−1). Fish were exposed to 0.5 mg l−1 of the formulated herbicide for 7, 30, and 90 days. Fish were exposed to clomazone in field conditions (7, 30, or 90 days trapped in submersed cages together with rice crops) and in laboratory conditions where the fish were placed in 45-l tanks with tap water only for 7 days. Fish exposed for 7, 30, or 90 days showed no alterations in acetylcholinesterase (AChE) activity under field conditions. Under laboratory conditions, decreased muscle AChE activity was observed only after 7 days of exposure. During the same evaluation period (7 days), oxidative stress parameters changed under both field and laboratory conditions; however, metabolic parameters were altered only under field conditions. Disorders in oxidative stress parameters and metabolism were evident in different tissues up to day 90 after treatment. These overall results show that AChE activity changed only under laboratory conditions. Oxidative stress, along with metabolic parameters, may be good indicators of herbicide contamination in C. carpio under rice-field conditions.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  • Almroth BC, Sturve J, Berglund A, Förlin L (2005) Oxidative damage in eelpout (Zoarces viviparus), measured as protein carbonyls and TBARS, as biomarkers. Aquat Toxicol 73:171–180

    Article  CAS  Google Scholar 

  • Almroth BC, Sturve J, Stephensen E, Holth TF, Förlin L (2008) Protein carbonyls and antioxidant defenses in corkwing wrasse (Symphodus melops) from a heavy metal polluted and a PAH polluted site. Mar Environ Res 66:271–277

    Article  CAS  Google Scholar 

  • Bainy ACD, Saito E, Carvalho PSM, Junqueira VBC (1996) Oxidative stress in gill, erythrocytes, liver and kidney of Nile tilapia (Oreochromis niloticus) from a polluted site. Aquat Toxicol 34:151–162

    Article  CAS  Google Scholar 

  • Ballesteros ML, Wunderlin DA, Bistoni MA (2009) Oxidative stress responses in different organs of Jenynsia multidentata exposed to endosulfan. Ecotoxicol Environ Safe 72:199–205

    Article  CAS  Google Scholar 

  • Begum G, Vijayaraghavan S (1999) Effect of acute exposure of the organophosphate insecticide rogor on some biochemical aspects of Clarias batrachus (Linnaeus). Environ Res 80:80–83

    Article  CAS  Google Scholar 

  • Bidinotto PM, Moraes G, Souza RHS (1998) Hepatic glycogen and glucose in eight tropical freshwater teleost fish: a procedure for field determinations of micro samples. Bol Téc CEPTA 10:53–60

    Google Scholar 

  • Bradford MM (1976) A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254

    Article  CAS  Google Scholar 

  • Buege JA, Aust SD (1978) Microsomal lipid peroxidation. Method Enzymol 52:302–309

    Article  CAS  Google Scholar 

  • Cardoso LM, Colombari DSA, Menani JV, De Paula PM, Chianca DA, Colombari E (2006) Espécies reativas de oxigênio no controle neurovegetativo da pressão arterial. Medicina 39(1):77–88

    Google Scholar 

  • Castagnolli N, Cyrino JEP (1986) Piscicultura nos trópicos. Manole LTDA, São Paulo, Brazil

    Google Scholar 

  • Crestani M, Menezes C, Glusczak L, Miron DS, Lazzari R, Duarte MF, Morsch VM et al (2006) Effects of clomazone herbicide on hematological and some parameters of protein and carbohydrate metabolism of silver catfish Rhamdia quelen. Ecotoxicol Environ Safe 65:48–55

    Article  CAS  Google Scholar 

  • Crestani M, Menezes C, Glusczak L, Miron DS, Spanevello R, Silveira A et al (2007) Effect of clomazone herbicide on biochemical and histological aspects of silver catfish (Rhamdia quelen) and recovery pattern. Chemosphere 67:2305–2311

    Article  CAS  Google Scholar 

  • Dorval J, Hontela A (2003) Role of glutathione redox cycle and catalase in defense against oxidative stress induced by endosulfan in adrenocortical cells of rainbow trout (Oncorhynchus mykiss). Toxicol Appl Pharm 92:191–200

    Article  Google Scholar 

  • Ellman GL, Courtney KD, Andres V Jr (1961) A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem Pharmacol 7:88–95

    Article  CAS  Google Scholar 

  • Ferrari A, Venturino A, Pechén de D’Angelo AM (2007) Effects of carbaryl and azinphos methyl on juvenile rainbow trout (Oncorhynchus mykiss) detoxifying enzymes. Pest Biochem Physiol 88:134–142

    Article  CAS  Google Scholar 

  • Fonseca MB, Glusczak L, Moraes BS, Menezes CC, Pretto A, Tierno MA et al (2008) 2,4-D herbicide effects on acetylcholinesterase activity and metabolic parameters of piava freshwater fish (Leporinus obtusidens). Ecotoxicol Environ Safe 69:416–420

    Article  Google Scholar 

  • Glusczak L, Miron DS, Crestani M, Fonseca MB, Pedron FA, Duarte MF et al (2006) Effect of glyphosate herbicide on acetylcholinesterase activity and metabolic and hematological parameters in piava (Leporinus obtusidens). Ecotoxicol Environ Safe 65:237–241

    Article  CAS  Google Scholar 

  • Glusczak L, Miron DS, Moraes BS, Simões RR, Schetinger MRC, Morsch VM et al (2007) Acute effects of glyphosate herbicide on metabolic and enzymatic parameters of silver catfish (Rhamdia quelen). Comp Biochem Physiol 146:519–524

    Google Scholar 

  • Habig WH, Pabst MJ, Jakoby WB (1974) Glutathione S-transferases. The first enzymatic step in mercapturic acid formation. J Biol Chem 249:7130–7139

    CAS  Google Scholar 

  • Harrower JR, Brown CH (1972) Blood lactic acid, a micromethod adapted to field collection of microliter samples. J Appl Physiol 32:709–711

    CAS  Google Scholar 

  • Lowry DH, Rosenbrough NJ, Farr AL, Randall RJ (1951) Protein measurement with Folin phenol reagent. J Biol Chem 193:265–275

    CAS  Google Scholar 

  • Marchesan E, Zanella R, Avila LA, Camargo ER, Machado SLO, Macedo VRM (2007) Rice herbicide monitoring in two Brazilian rivers during the rice growing season. Sci Agric 64:176–180

    Article  Google Scholar 

  • Menezes CC, Fonseca MB, Loro VL, Santi A, Cattaneo R, Clasen B, Pretto A, Morsch VM (2011) Roundup effects on oxidative stress parameters and recovery pattern of Rhamdia quelen. Arch Environ Contam Toxicol 60(4):665–671

    Article  CAS  Google Scholar 

  • Miron D, Crestani M, Schetinger MR, Morsch VM, Baldisserotto B, Tierno MA et al (2005) Effects of the herbicides clomazone, quinclorac, and metsulfuron methyl on acetylcholinesterase activity in the silver catfish (Rhamdia quelen) (Heptapteridae). Ecotoxicol Environ Safe 61:398–403

    Article  CAS  Google Scholar 

  • Miron DS, Pretto A, Crestani M, Glusczak L, Shettinger MR, Loro VL et al (2008) Biochemical effects of clomazone herbicide on piava (Leporinus obtusidens). Chemosphere 74:1–5

    Article  CAS  Google Scholar 

  • Moraes BS, Loro VL, Glusczak L, Pretto A, Menezes C, Marchezan E et al (2007) Effects of four rice herbicides on some metabolic and toxicology parameters of teleost fish (Leporinus obtusidens). Chemosphere 68:1597–1601

    Article  CAS  Google Scholar 

  • Moraes BS, Loro VL, Fonseca MB, Menezes CC, Marcehsan E, Reimche GB et al (2009) Toxicological and metabolic parameters of the teleost fish (Leporinus obtusidens) in response to commercial herbicides containing clomazone and propanil. Ecotoxicol Environ Safe 95:57–62

    CAS  Google Scholar 

  • Moraes BS, Clasen B, Loro VL, Pretto A, Toni C, Ávila LA et al (2011) Toxicological responses of Cyprinus carpio after exposure to a commercial herbicide containing imazethapyr and imazapic. Ecotoxicol Environ Safe 74:328–335

    Article  CAS  Google Scholar 

  • Nelson DP, Kiesow LA (1972) Enthalpy of decomposition of hydrogen peroxide by catalase at 25°C (with molar extinction coefficients of H2O2 solution in the UV). Anal Biochem 49:474–478

    Article  CAS  Google Scholar 

  • Nordberg J, Arnér ESJ (2001) Reactive oxygen species, antioxidants, and the mammalian thioredoxin system. Free Radic Biol Med 31:1287–1312

    Article  CAS  Google Scholar 

  • Oruç EO, Usta D (2007) Evaluation of oxidative stress responses and neurotoxicity potential of diazinon in different tissues of Cyprinus carpio. Environ Toxicol Pharmacol 23:48–55

    Article  Google Scholar 

  • Park JT, Johnson MJ (1949) A submicro determination of glucose. J Biol Chem 181:149–151

    CAS  Google Scholar 

  • Parvez S, Raisuddin S (2005) Protein carbonyls: Novel biomarkers of exposure to oxidative stress-inducing pesticides in freshwater fish Channa punctata (Bloch). Environ Toxicol Pharmacol 20:112–117

    Article  CAS  Google Scholar 

  • Parvez S, Pandey S, Ali M, Raisuddin S (2006) Biomarkers of oxidative stress in Wallago attu (Bl. and Sch.) during and after a fish-kill episode at Panipat, India. Sci Total Environ 368:627–636

    Article  CAS  Google Scholar 

  • Peixoto F, Alves-Fernandes D, Santos D, Fontaínhas-Fernandes A (2006) Toxicological effects of oxyfluorfen on oxidative stress enzymes in tilapia Oreochromis niloticus. Pest Biochem Phys 85:91–96

    Article  CAS  Google Scholar 

  • Rodrigues BN, Almeida FS (2005) Guide to herbicide, 5th edn. IAPAR, Londrina, pp 461–466

    Google Scholar 

  • Senseman SA (2007) Herbicide handbook, 9th edn. Weed Science Society of America, Lawrence

  • Spies JR (1957) Colorimetric procedures for amino acids. Method Enzymol 3:467–477

    Article  Google Scholar 

  • Verdouw H, Vanechteld CJA, Deckkers EMJ (1978) Ammonia determinations based on indophenol formation with sodium salicylate. Water Res 12:399–402

    Article  CAS  Google Scholar 

  • Yan LJ, Traber MG, Packer L (1995) Spectrophotometric method for determination of carbonyls in oxidatively modified apolipoprotein B of human low-density lipoproteins. Anal Biochem 228:349–351

    Article  CAS  Google Scholar 

  • Yi MQ, Liu HX, Shi XY, Liang P, Gao XW (2006) Inhibitory effects of four carbamate insecticides on acetylcholinesterase of male and female Carassius auratus in vitro. Comp Biochem Physiol C 143:113–116

    Article  CAS  Google Scholar 

  • Zanella R, Primel EG, Machado SLO, Gonçalves FF, Marchezan E (2002) Monitoring of the herbicide clomazone in environmental water samples by solid-phase extraction and high-performance liquid chromatography with ultraviolet detection. Chromatographia 55:573–577

    Article  CAS  Google Scholar 

  • Zhang J, Shen H, Wang X, Wu J, Xue Y (2004) Effects of chronic exposure of 2,4-dichlorophenol on the antioxidant system in liver of freshwater fish Carassius auratus. Chemosphere 55:167–174

    Article  CAS  Google Scholar 

Download references

Acknowledgments

We thank the Universidade Federal de Santa Maria for support and the facilities; CNPq/CT-HIDRO 552546/2007-09 for financial support; and Coordenação de aperfeiçoamento de pessoal de nível superior (CAPES) for the scholarship and the undergraduate student grant.

Author information

Authors and Affiliations

  1. Laboratório de Bioquímica Adaptativa, Departamento de Química, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil

    Roberta Cattaneo, Bibiana Silveira Moraes, Vania Lucia Loro, Alexandra Pretto, Charlene Menezes & Bárbara Clasen

  2. Departamento de Fitossanidade, UFPel, Pelotas, RS, Brazil

    Gerson Meneghetti Sarzi Sartori, Luis Antonio de Avila & Enio Marchesan

  3. Laboratório de Análises de Resíduos de Pesticidas, UFSM, Santa Maria, RS, Brazil

    Renato Zanella

Authors
  1. Roberta Cattaneo
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Bibiana Silveira Moraes
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Vania Lucia Loro
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Alexandra Pretto
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Charlene Menezes
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Gerson Meneghetti Sarzi Sartori
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Bárbara Clasen
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Luis Antonio de Avila
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Enio Marchesan
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Renato Zanella
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Luis Antonio de Avila.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Cattaneo, R., Moraes, B.S., Loro, V.L. et al. Tissue Biochemical Alterations of Cyprinus carpio Exposed to Commercial Herbicide Containing Clomazone Under Rice-Field Conditions. Arch Environ Contam Toxicol 62, 97–106 (2012). https://doi.org/10.1007/s00244-011-9669-8

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00244-011-9669-8

Keywords

Search

Navigation