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Biochemical Responses in Freshwater Fish Exposed to Insecticide Propoxur

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Abstract

Although designed to control pests selectively, there is some evidence that environmental contamination by pesticides increases risks for humans and wildlife. In the present study, we evaluated biomarkers of oxidative stress in Astyanax jacuhiensis exposed to (5, 15 and 30 µg L−1) of carbamate Propoxur (PPX) for 96 h. Glutathione S-transferase (GST) in liver and gills showed reduced activity in all PPX concentrations tested. Acetylcholinesterase (AChE) activities reduced in brain and muscle at concentrations 15 and 30 µg L−1 of PPX. Lipid peroxidation (LPO) and hydrogen peroxide (HP) had no significant differences. In the brain, protein carbonyl (PC) increased in all groups treated with PPX. Although PPX is a selective pesticide, it causes oxidative damage and enzyme alteration in fish. This study pointed out some biomarkers that could be used to assess effects of environmentally relevant concentrations of pesticides, and infer about studies using fish as bioindicator.

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Acknowledgements

We would like to thank Universidade Federal de Santa Maria (UFSM) for the support and the financial support and fellowships from the Brazilian agencies CAPES (Coordination for the Improvement of Higher Education Personnel) and CNPq (National Council for Scientific and Technological Development) for research´s fellowship to Vania Lucia Loro.

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Authors and Affiliations

  1. Programa de Pós-Graduação em Biodiversidade Animal, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil

    Carjone Rosa Gonçalves, Aline Teixeira Marins, Aline Monique Blank do Amaral, Eduardo Stringini Severo, Bibiana Silveira Moraes & Vania Lucia Loro

  2. Programa de Pós-Graduação em Ciências Biológicas – Bioquímica Toxicológica, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil

    Jossiele Leitemperger & Vania Lucia Loro

  3. Laboratório de Análises de Resíduos de Pesticidas (LARP), Universidade Federal de Santa Maria, Santa Maria, RS, Brazil

    Renato Zanella

  4. Laboratório de Toxicologia Aquática, Universidade Federal de Santa Maria, Santa Maria, Brazil

    Vania Lucia Loro

Authors
  1. Carjone Rosa Gonçalves
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  2. Aline Teixeira Marins
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  3. Aline Monique Blank do Amaral
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  4. Jossiele Leitemperger
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  5. Eduardo Stringini Severo
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  6. Bibiana Silveira Moraes
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  7. Renato Zanella
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  8. Vania Lucia Loro
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Correspondence to Vania Lucia Loro.

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Gonçalves, C.R., Marins, A.T., do Amaral, A.M.B. et al. Biochemical Responses in Freshwater Fish Exposed to Insecticide Propoxur. Bull Environ Contam Toxicol 100, 524–528 (2018). https://doi.org/10.1007/s00128-018-2285-9

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  • DOI: https://doi.org/10.1007/s00128-018-2285-9

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