Abstract
In this review, we introduce a new concept, precision toxicology: the mode of action of chemical- or drug-induced toxicity can be sensitively and specifically investigated by isolating a small group of cells or even a single cell with typical phenotype of interest followed by a single cell sequencing-based analysis. Precision toxicology can contribute to the better detection of subtle intracellular changes in response to exogenous substrates, and thus help researchers find solutions to control or relieve the toxicological effects that are serious threats to human health. We give examples for single cell isolation and recommend laser capture microdissection for in vivo studies and flow cytometric sorting for in vitro studies. In addition, we introduce the procedures for single cell sequencing and describe the expected application of these techniques to toxicological evaluations and mechanism exploration, which we believe will become a trend in toxicology.
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This work was supported by the National Special Program of Transgenic Research (Grant No. 2016ZX08011-005). The authors thank Beijing Advanced Innovation Center for Food Nutrition and Human Health for their kind support of this work.
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Zhang, B., Huang, K., Zhu, L. et al. Precision toxicology based on single cell sequencing: an evolving trend in toxicological evaluations and mechanism exploration. Arch Toxicol 91, 2539–2549 (2017). https://doi.org/10.1007/s00204-017-1971-4
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DOI: https://doi.org/10.1007/s00204-017-1971-4