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Moraxella haemolytica sp. nov., isolated from a goat with respiratory disease

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Abstract

An aerobic, haemolytic, Gram-negative and rod-shaped bacterial strain ZY171148T was isolated from the lung of a dead goat with respiratory disease in Southwest China. The strain grew at 24–39 °C, at pH 6.0–9.0 and in the presence of 0.5–2.0% (w/v) NaCl. Phylogenetic analysis of 16S rRNA gene sequences showed that the strain belongs to the genus Moraxella. The nucleotide sequence similarity analysis of the 16S rRNA gene showed that the strain has the highest similarity of 98.1% to Moraxella (M.) caprae ATCC 700019 T. Phylogenomic analysis of 800 single-copy protein sequences indicated that the strain is a member of the genus Moraxella and forms a separated branch on the Moraxella phylogenetic tree. The strain exhibited the highest orthologous average nucleotide identity (OrthoANI) and average amino acid identity (AAI) values of 77.0 and 77.9% to M. nasibovis CCUG 75921T and M. ovis CCUG 354T, respectively. The strain shared the highest digital DNA-DNA hybridization (dDDH) value of 26.2% to M. osloensis CCUG 350T. The genome G + C content of strain ZY171148T was 42.6 mol%. The strain had C18:1 ω9c (41.7%), C18:0 (11.2%), C16:0 (14.1%) and C12:0 3OH (9.7%) as the predominant fatty acids and CoQ-8 as the major respiratory quinone. The strain contained phosphatidylglycerol, phosphatidylethanolamine, cardiolipin, dilysocardiolipin, monolysocardiolipin and phosphatidic acid as the major polar lipids. β-haemolysis was observed on Columbia blood agar. All results confirmed that strain ZY171148T represents a novel species of the genus Moraxella, for which the name Moraxella haemolytica sp. nov. is proposed, with strain ZY171148T = CCTCC AB 2021471T = CCUG 75920T as the type strain.

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Funding

This research was supported by the Major Science and Technology Special Project from Yunnan Province Science and Technology Department (202102AE090039).

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Authors and Affiliations

  1. Yunnan Tropical and Subtropical Animal Virus Diseases Laboratory, Yunnan Animal Science and Veterinary Institute, Kunming, 650224, People’s Republic of China

    Fu-Xiang Li, Wen-Hua Zhao, Zhan-Hong Li, Jian-Ling Song & Hua-Feng Gao

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  1. Fu-Xiang Li
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  2. Wen-Hua Zhao
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  3. Zhan-Hong Li
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  4. Jian-Ling Song
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  5. Hua-Feng Gao
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Contributions

FL isolated the strain ZY171148T, supervised this study and wrote the manuscript; WZ, ZL and HG performed the tests; JS finished the phylogenetic and phylogenomic analysis. All the authors have reviewed this manuscript and agreed for publication.

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Correspondence to Fu-Xiang Li.

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The authors declare that there are no conflicts of interest.

Ethical approval

The lung tissue sampling from the dead goat with respiratory disease was approved by the Animal Ethics Committee of the Yunnan Animal Science and Veterinary Institute, and complied with the animal ethical standards of China.

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Communicated by Yusuf Akhter.

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Li, FX., Zhao, WH., Li, ZH. et al. Moraxella haemolytica sp. nov., isolated from a goat with respiratory disease. Arch Microbiol 206, 45 (2024). https://doi.org/10.1007/s00203-023-03782-8

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  • DOI: https://doi.org/10.1007/s00203-023-03782-8

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