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Moraxella nasibovis sp. nov., Isolated from a Cow with Respiratory Disease

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Abstract

Strain ZY190618T, isolated from the nasal cavity of a cow with respiratory disease, was subjected to taxonomic characterization. Cells of the strain were Gram-stain-negative, aerobic and coccus-shaped. Phylogenetic analysis based on 16 S rRNA gene sequences indicated that the strain belonged to the genus Moraxella with the highest similarity of 98.1% to Moraxella nasovis CCUG 75922T. Phylogenomic analysis based on 810 single-copy genes revealed that the strain was a member of the genus Moraxella and formed a deep and separated clade within the genus. The strain showed the highest orthologous average nucleotide identity (OrthoANI) value of 77.1% with Moraxella ovis CCUG 354T and digital DNA-DNA hybridization (dDDH) value of 24.7% with Moraxella equi NCTC 11012T, respectively. The DNA G + C content was 46.5 mol%. The strain optimally grew at 37 °C (temperature range, 24–42 °C), at pH 8.0 (pH range, 6.0–9.0) and with 1.5% (w/v) NaCl (NaCl range, 0.5–3.0%). The strain contained C18:1 ω9c as the sole predominant fatty acid (> 5 %) and CoQ-8 as the major respiratory quinone. The major polar lipids included phosphatidylglycerol, phosphatidylethanolamine, cardiolipin, monolysocardiolipin and hemibismonoacylglycerophosphate. Based on these data, strain ZY190618T clearly represents a novel species in the genus Moraxella, for which the name Moraxella nasibovis sp. nov. (The type strain ZY190618T = CCUG 75921T = CCTCC AB 2021472T) is proposed.

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Acknowledgements

Professor Aharon Oren (The Hebrew University of Jerusalem) is gratefully acknowledged for nomenclature of the novel species.

Funding

This research was funded by the Major Science and Technology Special Project of Yunnan Province (202102AE090039).

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Authors and Affiliations

  1. Yunnan Tropical and Subtropical Animal Virus Diseases Laboratory, Yunnan Animal Science and Veterinary Institute, Kunming, 650224, People’s Republic of China

    Fuxiang Li, Wenhua Zhao, Pei Zhu, Zhanhong Li, Jianling Song, Jianbo Zhu & Huafeng Gao

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  1. Fuxiang Li
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  2. Wenhua Zhao
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  3. Pei Zhu
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  4. Zhanhong Li
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Contributions

FL isolated the strain ZY190618T and wrote the manuscript; PZ, ZL and WZ performed the experiments; JZ and JS carried out the data analysis; HG supervised the study. The manuscript has been reviewed and approved by all authors.

Corresponding author

Correspondence to Huafeng Gao.

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Ethical Approval

All procedures involved in the nasal swab sampling from cows were approved by Animal Ethics Committee of the Yunnan Animal Science and Veterinary Institute (No. 19 − 003), and complied with animal ethical standards of China.

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The authors declare that there are no conflicts of interest.

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The GenBank/EMBL/DDBJ/PIR accession numbers for the 16 S rRNA gene and complete genome sequences of strain ZY190618T are OL841892 and CP089975, respectively.

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Li, F., Zhao, W., Zhu, P. et al. Moraxella nasibovis sp. nov., Isolated from a Cow with Respiratory Disease. Curr Microbiol 80, 305 (2023). https://doi.org/10.1007/s00284-023-03415-9

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