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Moraxella nasicaprae sp. nov., Isolated from a Goat with Respiratory Disease

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Abstract

A novel Gram-stain-negative, aerobic, irregular coccus designated as ZY201224T, was isolated from the nasal cavity of a goat with respiratory disease in a goat farm, located at Jianshui, Yunnan Province, PR China and its taxonomic position was clarified using a polyphasic approach. The strain grew optimally at 37 °C, at pH 8.0 and in the presence of 1% NaCl. Phylogenetic analysis based on 16S rRNA gene sequence and phylogenomic analysis based on 808 single-copy genes revealed that the strain is affiliated to the genus Moraxella and is distinct from the recognized species of the genus. The 16S rRNA gene sequence similarity analysis indicated that the strain is most closely related to Moraxella caviae CCUG 355T with sequence similarity of 98.1%. The genomic OrthoANI and digital DNA-DNA hybridization (dDDH) values between the strain and the type strains of Moraxella species were no higher than 74.7% (Moraxella pluranimalium CCUG 54913T) and 26.0% (Moraxella oblonga NBRC 102422T), respectively. The G + C content of the complete genome sequence was 43.6 mol%. The strain contained CoQ-8 as the major respiratory quinone, and C18:1ω9c, C17:1ω8c, C16:0 and summed feature 3 (C16:1 ω7c and/ or C16:1ω6c) as the predominant fatty acids (> 5%). The major polar lipids comprised phosphatidylglycerol (PG), cardiolipin (CL), monolysocardiolipin (MLCL), phosphatidylethanolamine (PE) and lysophosphatidylglycerol (LPG). Based on these taxonomic characterizations, strain ZY201224T represents a novel species of the genus Moraxella, for which the name Moraxella nasicaprae sp. nov. is proposed. The type strain is ZY201224T (= CCTCC AB 2021474T = NBRC 115473T).

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Acknowledgements

We thank Professor Aharon Oren from The Hebrew University of Jerusalem (Israel) for helping in nomenclature of the novel species.

Funding

This work was supported by a grant from the Major Science and Technology Special Project of Yunnan Province (202102AE090039).

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Authors and Affiliations

  1. Yunnan Tropical and Subtropical Animal Virus Diseases Laboratory, Yunnan Animal Science and Veterinary Institute, Kunming, 650224, People’s Republic of China

    Fuxiang Li, Huafeng Gao, Pei Zhu, Zhanhong Li, Wenhua Zhao, Jianling Song & Shibiao Yang

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Contributions

FL: isolated the strain ZY201224T, performed the chemotaxonomic analysis and wrote the manuscript. PZ, ZL and HG: performed the physiological experiments. WZ and JS: carried out the phylogenetic analysis. SY: supervised the study and revised the manuscript. All authors have read and approved the manuscript.

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Correspondence to Shibiao Yang.

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Permission for sampling the nasal swabs was granted by the farm owner. All procedures performed in this studies involving goat was approved by Animal Ethics Committee of the Yunnan Animal Science and Veterinary Institute, and were in accordance with Animal Ethics Laws and Regulations in P. R. China.

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Li, F., Gao, H., Zhu, P. et al. Moraxella nasicaprae sp. nov., Isolated from a Goat with Respiratory Disease. Curr Microbiol 80, 78 (2023). https://doi.org/10.1007/s00284-023-03185-4

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