Abstract
A novel aerobic bacterium, strain PS-22 of the genus Moraxella, was isolated from the skin of freshwater pufferfish (Tetraodon cutcutia). Cells were Gram stain negative, aerobic, non-motile, and coccoid. Optimum growth occurred at 28–30 °C and pH 6.5–7.5. The major cellular fatty acids were C18:1 ω9c, C10:0, C16:0, and C12:0 anteiso. The predominant polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phospholipid, amino lipid, and seven unknown lipids. The genome size is 2.68 Mbp, and the DNA G + C content was 43.3%. A gene ontology study revealed that the major fraction of genes were associated with biological processes (46.81%), followed by molecular function (34.27%) and cellular components (18.8%). Comparisons of 16S rRNA gene sequences revealed 99.11–90% sequence similarity with the closely related type strains of the genus Moraxella. The average nucleotide identity (ANI) and average amino acid identity (AAI) of strain PS-22 with reference type strains of the genus Moraxella were below 95–96%, and the corresponding in silico DNA–DNA hybridization (DDH) values were below 70%. A phylogenetic tree based on genome-wide core genes and 16S rRNA gene sequences revealed that strain PS-22 clustered with Moraxella osloensis CCUG350T in both the phylogenetic trees. Genotypic and phenotypic characteristics of strain PS-22 represent a novel species for which Moraxella tetraodonis sp. nov. is proposed. The type strain is PS-22T (= TBRC 15232T = NBRC 115236T).
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Acknowledgements
The author LD acknowledges the Council of Scientific and Industrial Research (CSIR), New Delhi, Government of India for providing the research fellowship. We acknowledge the Distributed Information Sub-Center (DISC) at Institute of Life Sciences, Bhubaneswar for the computational facility. We are thankful to Professor B. Schink (Universitaet Konstanz, Germany) for etymological advice.
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This work was supported in part by the funding received from Department of Biotechnology, Government of India (D.O.No. BT/PR7661/AAQ/3/629/2013) to SKD.
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SKD: designed the study. LD: conducted the experiment. LD and SKD: analysed the data and wrote the manuscript.
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This study was carried out with approval from the Institutional Animal Ethics Committee (Letter No. V-311-MISC/2017-18/ILS/884).
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Das, L., Das, S.K. Moraxella tetraodonis sp. nov., isolated from freshwater pufferfish (Tetraodon cutcutia) skin. Arch Microbiol 204, 389 (2022). https://doi.org/10.1007/s00203-022-03013-6
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DOI: https://doi.org/10.1007/s00203-022-03013-6